Microbiology Core

微生物学核心

• 批准号: 8089288
• 负责人: Hao Shen
• 金额: $ 17.26万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-01 至 2014-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8089288
• 关键词: Address; Animals; Antiviral Agents; B-Lymphocytes; Bacteria; Bacterial Infections; Bacterial Pneumonia; Bacteriophages; Biological Assay; CD4 Positive T Lymphocytes; Complication; Data; Development; Effectiveness; Ensure; Experimental Designs; Future; Goals; Human; Immunity; Immunologic Memory; Individual; Infection; Infectious Agent; Inflammation; Location; Microbiology; Modeling; Monitor; Morbidity - disease rate; Procedures; RNA Viruses; Recombinants; Regulatory T-Lymphocyte; Reproducibility; Research Project Grants; Respiratory System; Respiratory Tract Infections; Respiratory tract structure; Shapes; Standardization; Streptococcus pneumoniae; T-Lymphocyte; Testing; Tissues; Vaccines; Viral; Viral Load result; Virus; Virus Diseases; improved; influenza virus strain; influenza virus vaccine; influenzavirus; mortality; novel; pandemic disease; pandemic influenza; pathogen; programs; respiratory; tool; vaccination strategy

Viral infections remain a considerable cause of morbidity and mortality. Despite advances in the availability of vaccines, the mechanisms of generating and maintaining effective antiviral immunity remains incompletely understood. A major concern in this regard is influenza virus, a focal point for a number of Projects in this Program. The vaccine for influenza virus must be remade each year because it has not yet been possible to generate broadly protective immunity to this rapidly evolving RNA virus and the potential emergence of devastating pandemic influenza virus strains remains ever present. Thus, it is essential that we understand the basic principles of long-term B cell and T cell immunity to viral infections, including influenza virus, to improve existing, and develop novel, vaccination strategies. A major complication with influenza virus infection is co-infection with bacteria resulting in bacterial pneumonia. It is unclear how such a co-infection with a virus and bacterial pathogen in the respiratory tract impacts antiviral immunological memory and future protective immunity to the virus. To address these questions, the research Projects will utilize common infectious agents. The overall goal of Core B is to generate, standardize, store and provide infectious agents to the Projects. In addition, Core B will optimize and validate assays to monitor the burden of infectious agents in tissues from infected animals. Thus, the specific Aims for this core are: 1) To define a model of respiratory infection with Streptococcus pneumonia (Sp) and coinfection with Sp and influenza virus; 2) To generate and characterize Streptococcus pneumoniae expressing CD4 T cell determinant(s) from influenza virus HA; and 3) To produce, standardize, maintain and store infectious agents. The activities of this Core will therefore create synergy and interaction between the different Projects by creating a common and easily accessible set of tools, and by enhancing the quality, standardization and reproducibility of the data generated by the individual Projects.

病毒感染仍然是发病率和死亡率的重要原因。尽管在可用性方面取得了进展 在疫苗中，产生和维持有效的抗病毒免疫的机制仍然不完全。 明白了。这方面的一个主要关切是流感病毒，它是这方面一些项目的焦点。 程序。流感病毒疫苗必须每年重新制造，因为目前还不可能 对这种快速进化的RNA病毒产生广泛的保护性免疫力，并可能出现 毁灭性的大流行性流感病毒株仍然存在。因此，我们必须了解 B细胞和T细胞对包括流感病毒在内的病毒感染的长期免疫的基本原理 改进现有的疫苗接种策略，并开发新的疫苗接种策略。流感病毒的主要并发症 感染是指与细菌混合感染，导致细菌性肺炎。目前还不清楚这种联合感染是如何发生的。 呼吸道中的病毒和细菌病原体会影响抗病毒免疫记忆和 未来对病毒的保护性免疫。为了解决这些问题，研究项目将利用 常见的传染病病原体。核心B的总体目标是生成、标准化、存储和提供 传染给项目的病原体。此外，Core B将优化和验证检测以监控负担 来自受感染动物的组织中的感染性病原体。因此，这个核心的具体目标是：1)定义一个 肺炎链球菌呼吸道感染及其与流感混合感染模型的建立 病毒；2)表达CD4T细胞决定簇的肺炎链球菌的建立和鉴定(S) 来自流感病毒HA；以及3)生产、标准化、维护和储存传染病病原体。活动 因此，该核心将通过创建一个 一套通用和易于使用的工具，并通过提高质量、标准化和可再现性 由各个项目生成的数据。