STUDY OF EQUILIBRIUM AND NON-EQUILIBRIUM DYNAMICS BY 2D IR

用二维红外研究平衡和非平衡动力学

基本信息

  • 批准号:
    8169537
  • 负责人:
  • 金额:
    $ 16.62万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2010
  • 资助国家:
    美国
  • 起止时间:
    2010-06-01 至 2011-05-31
  • 项目状态:
    已结题

项目摘要

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The answers to many questions in biology require knowledge of structures that are undergoing changes between states on time scales not accessible by the powerful structural biology methods of x-ray diffraction and NMR. Kinetic spectroscopy approaches tell us about the rates at which populations interchange and hence about mechanisms. However the range of equilibrium configurations and the structures of the various intermediate states cannot always be determined by these standard methods. In our work we propose new methods to determine structural aspects of these populations and their distributions regardless of how fleeting they are. Such measurements are expected to provide answers to questions regarding the evolution of segments of structures that are not available from other techniques. This is the significance of this work. Vibrational spectroscopy has provided important experimental access to the microscopic aspects of hydrogen bond dynamics in complex systems. The dynamics of OH or OD stretching vibrational modes in water or alcohol oligomers, the vibrations of molecular and atomic aqueous ions, and the NH and C=O stretching modes including those in peptides or proteins in water are very sensitive to and correlated with the structural and dynamical properties of hydrogen bonds. In principle, the shape of the conventional IR absorption spectrum provides information on the equilibrium dynamics of a hydrogen bonded system. However, in many cases the line shapes are determined by population lifetimes and spectral diffusion processes that often cannot be reduced to the unique set of parameters needed to describe the frequencies and amplititudes of coupled solvent nuclear motions. With the help of multidimensional nonlinear spectroscopic techniques in the IR, it has become possible to probe these hydrogen bond dynamics and extract more details on the structures and dynamics with high time resolution. Dynamical information on the OH, OD, and NH stretching modes of intermolecular hydrogen bonded systems has been obtained in the form of vibrational lifetimes, energy transfer, hydrogen bond breaking and reforming rates, and the time dependence of spectral diffusion. The motions of hydrogen bonds in peptides are of importance in biological processes. The amide carbonyl group is very often involved in dynamic hydrogen bonding either to water or to N-H groups or to both. Much remains to be learned from experiments on the vibrational populations and coherences about the dynamics of these hydrogen bonds for a wide range of environments. These chemical exchanges can be directly studied by 2D IR experiments that have wide applicability for the study of such ultrafast dynamics. Furthermore, for a full interpretation of 2D IR this knowledge of the dynamics is necessary. Major objectives of this core project are: - Methods of characterization by 2D IR of the equilibrium and nonequilibrium dynamics and the spatial variations of solvent structure and dynamics. - Development of methods of 2D IR temperature jump experiments applied to continuous structure evolution such as occurs in downhill protein folding reaction. - Methods of optical pump - IR and 2D IR probe spectroscopy to investigate the structures of reaction intermediates of proteins undergoing unfolding transitions. - Hydrogen bond exchange 2D IR methods and the interpretation of 2D IR line shapes including exchanges between vibrationally distinct groups of molecules. - Development of 2D IR methods to measure the frequency correlation functions of multi-amide unit peptides combined with molecular dynamics simulations, ab initio computations and structure/frequency maps. - Methods of dual frequency dynamics and determination of joint correlation functions of C-H, N-H, amide-II and amide-I modes. - Development of direct experimental approaches to expose solvation by dual frequency 2D IR experiments that excite the peptide backbone modes and probe the solvent motions that respond. - Generation of reliable approaches to the measurement and interpretation of vibrational energy transport along peptide backbones.
这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者(PI)可能从另一个NIH来源获得了主要资金, 因此可以在其他CRISP条目中表示。所列机构为 研究中心,而研究中心不一定是研究者所在的机构。 生物学中许多问题的答案需要了解在时间尺度上状态之间发生变化的结构,而X射线衍射和核磁共振等强大的结构生物学方法无法获得这些知识。动力学光谱学方法告诉我们种群交换的速率,从而告诉我们交换的机制。然而,平衡构型的范围和各种中间态的结构并不总是由这些标准方法确定。在我们的工作中,我们提出了新的方法来确定这些人口及其分布的结构方面,无论他们是多么短暂。这种测量有望为其他技术无法提供的结构片段演变问题提供答案。这就是这项工作的意义。 振动光谱学为复杂系统中氢键动力学的微观方面提供了重要的实验途径。O的动态H或O水或醇低聚物中的D伸缩振动模式,分子和原子水离子的振动,以及NH和C=O伸缩模,包括那些在肽或蛋白质在水中是非常敏感的,并与氢键的结构和动力学性质相关。原则上,常规IR吸收光谱的形状提供了关于氢键系统的平衡动力学的信息。然而,在许多情况下,线的形状是由人口寿命和光谱扩散过程,往往不能减少到唯一的一组参数,需要描述耦合溶剂核运动的频率和纬度。借助红外光谱中的多维非线性光谱技术,可以探测这些氢键动力学,并以高时间分辨率提取有关结构和动力学的更多细节。关于O的动态信息氢氧D和N以振动寿命、能量转移、氢键断裂和重组速率以及光谱扩散的时间依赖性等形式得到了分子间氢键体系的H伸缩模。肽中氢键的运动在生物过程中具有重要意义。酰胺羰基经常参与与水或N-H基团或两者的动态氢键合。还有很多东西要从实验中了解的振动人口和凝聚力的动力学,这些氢键的广泛的环境。这些化学交换可以直接研究二维红外实验,具有广泛的适用性,这样的超快动力学的研究。此外,对于2D IR的完整解释,这种动力学知识是必要的。 这一核心项目的主要目标是: - 平衡和非平衡动力学的二维红外表征方法以及溶剂结构和动力学的空间变化。 - 开发了适用于连续结构演变(如蛋白质折叠反应)的二维红外温度跃变实验方法。 - 光泵红外和二维红外探针光谱研究蛋白质去折叠跃迁反应中间体结构的方法。 - 氢键交换二维红外方法和二维红外线形状的解释,包括分子的振动不同基团之间的交换。 - 结合分子动力学模拟、从头计算和结构/频率图,开发了二维红外方法来测量多酰胺单元肽的频率相关函数。 - 双频动力学方法和C-H、N-H、酰胺-II和酰胺-I模式的联合相关函数的确定。 - 开发直接实验方法,通过双频2D IR实验激发肽骨架模式并探测响应的溶剂运动来暴露溶剂化。 - 产生可靠的方法来测量和解释振动能量传输沿着肽骨架。

项目成果

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ROBIN Main HOCHSTRASSER其他文献

ROBIN Main HOCHSTRASSER的其他文献

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{{ truncateString('ROBIN Main HOCHSTRASSER', 18)}}的其他基金

DEVELOPMENT OF METHODS OF SINGLE FREQUENCY 2D IR
单频二维红外方法的开发
  • 批准号:
    8362563
  • 财政年份:
    2011
  • 资助金额:
    $ 16.62万
  • 项目类别:
2D IR DUAL FREQUENCY AND DUAL ISOTOPE REPLACEMENT STRATEGIES
2D IR 双频和双同位素替代策略
  • 批准号:
    8362564
  • 财政年份:
    2011
  • 资助金额:
    $ 16.62万
  • 项目类别:
STUDY OF EQUILIBRIUM AND NON-EQUILIBRIUM DYNAMICS BY 2D IR
用二维红外研究平衡和非平衡动力学
  • 批准号:
    8362565
  • 财政年份:
    2011
  • 资助金额:
    $ 16.62万
  • 项目类别:
DISSEMINATION AND TRAINING
传播和培训
  • 批准号:
    8362570
  • 财政年份:
    2011
  • 资助金额:
    $ 16.62万
  • 项目类别:
2D IR DUAL FREQUENCY AND DUAL ISOTOPE REPLACEMENT STRATEGIES
2D IR 双频和双同位素替代策略
  • 批准号:
    8169536
  • 财政年份:
    2010
  • 资助金额:
    $ 16.62万
  • 项目类别:
DISSEMINATION AND TRAINING
传播和培训
  • 批准号:
    8169542
  • 财政年份:
    2010
  • 资助金额:
    $ 16.62万
  • 项目类别:
TECH R&D CORE SUPPORT FOR AIDS RESEARCH
技术研发
  • 批准号:
    8169554
  • 财政年份:
    2010
  • 资助金额:
    $ 16.62万
  • 项目类别:
DEVELOPMENT OF METHODS OF SINGLE FREQUENCY 2D IR
单频二维红外方法的开发
  • 批准号:
    8169535
  • 财政年份:
    2010
  • 资助金额:
    $ 16.62万
  • 项目类别:
DEVELOPMENT OF METHODS OF SINGLE FREQUENCY 2D IR
单频二维红外方法的开发
  • 批准号:
    7955429
  • 财政年份:
    2009
  • 资助金额:
    $ 16.62万
  • 项目类别:
Ultrafast Processing in Proteins and Other Assemblies
蛋白质和其他组装体的超快处理
  • 批准号:
    7932592
  • 财政年份:
    2009
  • 资助金额:
    $ 16.62万
  • 项目类别:

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受阻酰胺的天体选择性合成-合成肽催化剂的探索-
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