Genetic and Molecular Dissection of RanBP2-Mediated RanGTPase Functions

RanBP2 介导的 RanGTPase 功能的遗传和分子解析

• 批准号: 7984822
• 负责人: PAULO A FERREIRA
• 金额: $ 30.62万
• 依托单位: DUKE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-09-01 至 2014-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7984822
• 关键词: 1-Methyl-4-phenylpyridinium; Affinity; Age; Aging; Binding; Binding Proteins; Biogenesis; Cell Death; Cells; Cessation of life; Clinical; Development; Disease; Dissection; Figs - dietary; Goals; Guanosine Triphosphate Phosphohydrolases; Homeostasis; Impairment; Kinesin; Lead; Mediating; Mitochondria; Modeling; Molecular; Molecular Genetics; Mus; Nerve Degeneration; Neurologic; Neurons; Neurotoxins; Oxidative Stress; Pathogenesis; Phenotype; Photoreceptors; Process; Property; Protein Dynamics; Proteins; Regulation; Research Project Grants; Role; Running; Stress; System; Testing; Therapeutic Intervention; Transgenic Mice; Treatment Efficacy; Ubiquitin; age related; cell type; disease stressor; dopaminergic neuron; ganglion cell; high intraocular pressure; improved; loss of function; loss of function mutation; mouse model; multicatalytic endopeptidase complex; nervous system disorder; neuronal survival; neuroprotection; novel; protein degradation; public health relevance; sex; stressor; therapeutic target; trafficking

DESCRIPTION (provided by applicant): The deregulation of mitochondria dynamics and function of the ubiquitin-proteasome system (UPS) are hallmark features of the pathogenesis of numerous neurodegenerative and aging-related disorders causing severe neurological impairment. Current therapeutic interventions that promote neuroprotection lack therapeutic efficacy. Our long-term goal is to identify targets with novel neuroprotective properties and high therapeutic efficacies that delay the onset of or cure clinical manifestations causing neurological impairment. To attain this goal we propose a research project whose long-term objective is the development of improved understanding of the role of factors controlling mitochondria dynamics and UPS functions, the identification of cross-talk processes between these factors and processes, and the effect(s) of such factors and processes in modulation of neuronal survival. Understanding the role(s) of factors that control mitochondria dynamics or UPS activity and contribute to the modulation of neuronal survival, would allow us and others to create novel value targets and therapeutic strategies to delay or cure the development of clinical manifestations leading to severe neurological impairments. We will focus on determining the functional relationships between RAN GTPase and one of its high-affinity and multi-binding targets, the RAN-binding protein 2 (RANBP2). This proposal tests our overall hypothesis that the associations between RAN GTPase and, the RAN-binding domains-2 and -3 (RBD2 and RBD3) of RANBP2, constitute a novel molecular switch to control effector domains of RANBP2 in the modulation of UPS function, mitochondria dynamics by kinesin-1, and neuronal survival. We will test this hypothesis by accomplishing the following specific aims, which focus on the mechanistic roles of RanBP2 and its RBD2 and RBD3 in the regulation of mitochondria dynamics, protein homeostasis and survival of selective neurons under normal and disease stress conditions. Aim 1. Test the hypothesis that loss-of-function of the RBD2 or RBD3 of RANBP2 promotes differential effects in mitochondria trafficking and survival among neuronal cell types. Aim 2. Test the hypothesis that loss-of-function of the RBD3 of RANBP2 promotes differential effects in UPS activity among neuronal cell types. Aim 3. Test the hypothesis that impairment of RAN GTPase-dependent modulation of mitochondria trafficking or UPS activity promotes either neuroprotection or cell death of selective neurons in three mouse models of stress-induced neurodegeneration: light-induced death of photoreceptors, high intraocular pressure-induced ganglion cell death, and MPP+induced death of dopaminergic neurons. PUBLIC HEALTH RELEVANCE: The deregulation of trafficking of mitochondria and of the machinery controlling protein degradation underlies numerous neurological disorders leading to neurodegeneration. This study seeks to identify and elucidate the role of factors, such as RanGTPase and Ran-binding protein-2, in controlling mitochondria dynamics and protein degradation and survival of various types of neurons under normal and disease stress conditions.

描述（由申请人提供）：线粒体动力学和泛素-蛋白酶体系统（UPS）功能的失调是导致严重神经功能损害的许多神经退行性和衰老相关疾病发病机制的标志性特征。目前促进神经保护的治疗干预缺乏治疗功效。我们的长期目标是确定具有新的神经保护特性和高疗效的靶点，以延迟或治愈引起神经损伤的临床表现。为了实现这一目标，我们提出了一个研究项目，其长期目标是加深对控制线粒体动力学和UPS功能的因素的作用的了解，识别这些因素和过程之间的串扰过程，以及这些因素和过程的影响。调节神经元存活的过程。了解控制线粒体动力学或UPS活性并有助于调节神经元存活的因素的作用，将使我们和其他人能够创造新的价值目标和治疗策略，以延迟或治愈导致严重神经功能障碍的临床表现的发展。我们将专注于确定RAN GT3与其高亲和力和多结合靶点之一RAN结合蛋白2（RANBP 2）之间的功能关系。该提议验证了我们的总体假设，即RANBP 2的RAN结合结构域-2和-3（RBD 2和RBD 3）与RANBP 2的RAN结合结构域之间的关联构成了一种新的分子开关，以控制RANBP 2的效应结构域调节UPS功能、驱动蛋白-1的线粒体动力学和神经元存活。我们将通过实现以下具体目标来测试这一假设，这些目标集中在RanBP 2及其RBD 2和RBD 3在正常和疾病应激条件下调节线粒体动力学、蛋白质稳态和选择性神经元存活中的机制作用。目标1.检验RANBP 2的RBD 2或RBD 3的功能丧失促进神经元细胞类型之间线粒体运输和存活的差异效应的假设。目标2.检验RANBP 2的RBD 3功能丧失促进神经元细胞类型之间UPS活性的差异效应的假设。目标3.在三种应激诱导的神经变性小鼠模型中检验线粒体运输或UPS活性的RAN GTP酶依赖性调节受损促进选择性神经元的神经保护或细胞死亡的假设：光诱导的光感受器死亡、高眼内压诱导的神经节细胞死亡和MPP+诱导的多巴胺能神经元死亡。 公共卫生相关性：线粒体运输和控制蛋白质降解的机制的失调是导致神经变性的许多神经系统疾病的基础。本研究旨在确定和阐明的作用的因素，如RanGT和RAN结合蛋白-2，在控制线粒体动力学和蛋白质降解和正常和疾病应激条件下的各种类型的神经元的生存。