Differential regulation of plant innate immunity

植物先天免疫的差异调节

• 批准号: 7862794
• 负责人: Ping He
• 金额: $ 25.42万
• 依托单位: TEXAS A&M UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-04-01 至 2015-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7862794
• 关键词: Animals; Arabidopsis; Attenuated; Binding; Biochemical; Biological Assay; Biological Models; Biological Process; Calcium; Cell surface; Cells; Communicable Diseases; Complex; Data; Detection; Employee Strikes; Environment; Environmental Risk Factor; Event; Family; Family member; Figs - dietary; Gene Expression Profile; Gene Expression Regulation; Gene Family; Genetic; Genome; Genomics; Goals; Immune; Immune response; Immune system; Immunity; Individual; Infection; Leucine-Rich Repeat; Life; Mammals; Microbe; Molecular; Natural Immunity; Nucleotides; Pathogen detection; Pattern; Pattern recognition receptor; Phosphotransferases; Plant Model; Plants; Play; Regulation; Research; Resources; Role; Signal Pathway; Signal Transduction; System; Temperature; Toll-like receptors; Virulence; Work; base; calcium-dependent protein kinase; improved; leucine-rich repeat protein; member; novel; pathogen; prevent; public health relevance; receptor; sensor; tool

DESCRIPTION (provided by applicant): Plants and animals rely on innate immunity to prevent potential infections by detection of pathogen-associated molecular patterns (PAMPs) through pattern recognition receptors (PRRs). In plants, PAMPs are perceived by cell-surface PRRs and mount PAMP- triggered immunity (PTI). Successful pathogens are able to suppress PTI by producing virulence effectors, which, in turn, are recognized by plant intracellular PRRs to initiate effector-triggered immunity (ETI). This complex host-microbe interaction is intricately intertwined with a wide range of environmental factors. For example, we found that the ambient temperature fluctuation regulates PTI and ETI in a distinct manner. We have developed an Arabidopsis cell system to express individual pathogen effectors for ETI or treat cells with purified PAMPs for PTI. In combination with enriched genetic and genomic resources in Arabidopsis, this synchronized and autonomous plant cell-single pathogen signal system holds significant promise for dissecting the complex regulation of signaling events in ETI and PTI. Our preliminary data strongly suggest the existence of differential early signaling mechanisms underlying two branches of plant innate immunity. In particular, we found that the specific members of a large gene family encoding calcium-dependent protein kinases (CDPKs) play pivotal roles in transducing calcium signature in ETI. Our proposed research will employ a multifaceted approach to further understand the signaling mechanisms underlying plant ETI and PTI. The Specific Aims for this application are the following: 1) Decipher the distinct and convergent gene regulation in ETI and PTI; 2) Elucidate the functions of CDPKs in ETI signaling; 3) Dissect the ambient temperature regulation of innate immunity. Plant PTI and ETI are most similar to "Toll-like receptor" and "NOD-like receptor" innate immune signaling pathways in mammals respectively. Thus, our research will contribute to a general understanding of innate immunity and improve our ability to prevent and control infectious diseases. PUBLIC HEALTH RELEVANCE: Understanding the intricately intertwined signal transduction networks acting downstream of various host immune sensors could provide novel avenues to prevent and control infectious diseases. Given that plant cell-surface and intracellular immune sensors are most similar to "Toll-like receptor" and "NOD-like receptor" in mammals respectively, our research will have broad impact on the understanding of signaling mechanisms of animal innate immunity.

描述（由申请人提供）：植物和动物依靠先天免疫通过模式识别受体（PRR）检测病原体相关分子模式（PAMP）来预防潜在感染。在植物中，PAMP被细胞表面PRR感知并产生PAMP触发的免疫（PTI）。成功的病原体能够通过产生毒力效应子来抑制PTI，而毒力效应子又被植物细胞内PRR识别，以启动效应子触发的免疫（ETI）。这种复杂的宿主-微生物相互作用与广泛的环境因素错综复杂地交织在一起。例如，我们发现环境温度波动以不同的方式调节PTI和ETI。我们已经开发了一种拟南芥细胞系统，以表达ETI的单个病原体效应物或用纯化的PAMP处理细胞用于PTI。结合丰富的遗传和基因组资源，在拟南芥中，这种同步和自主的植物细胞单病原体信号系统具有重要的承诺，解剖复杂的调控ETI和PTI的信号事件。我们的初步数据有力地表明，存在差异的早期信号传导机制的两个分支的植物先天免疫。特别是，我们发现，一个大的基因家族编码的钙依赖性蛋白激酶（CDPKs）的特定成员在ETI的钙信号转导中发挥关键作用。我们提出的研究将采用多方面的方法来进一步了解植物ETI和PTI的信号机制。本申请的具体目的如下：1）破译ETI和PTI中不同和趋同的基因调控; 2）阐明CDPKs在ETI信号传导中的功能; 3）剖析天然免疫的环境温度调控。植物PTI和ETI分别与哺乳动物的“Toll样受体”和“NOD样受体”先天免疫信号通路最为相似。因此，我们的研究将有助于对先天免疫的全面了解，并提高我们预防和控制传染病的能力。 公共卫生相关性：了解各种宿主免疫传感器下游错综复杂的信号转导网络可以为预防和控制传染病提供新的途径。鉴于植物细胞表面和细胞内免疫传感器分别与哺乳动物中的“Toll样受体”和“NOD样受体”最为相似，我们的研究将对理解动物先天免疫的信号传导机制产生广泛的影响。