FUSE Binding Protein As a Cellular Effector of HCV Replication

FUSE 结合蛋白作为 HCV 复制的细胞效应子

• 批准号: 7788343
• 负责人: Virendra Nath PANDEY
• 金额: $ 19.5万
• 依托单位: UNIV OF MED/DENT OF NJ-NJ MEDICAL SCHOOL
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-07-01 至 2012-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7788343
• 关键词: Affect; Affinity; Binding; Binding Proteins; Cell physiology; Cells; Chronic Hepatitis C; Complex; DNA; Differentiation and Growth; Down-Regulation; Elements; Genes; Genetic Transcription; Genomics; Hepatitis; Hepatitis C virus; Liver; Liver Cirrhosis; Mediating; Molecular; Nature; Pathogenesis; Patients; Pattern; Primary carcinoma of the liver cells; Protein Microchips; Pyrimidine; Pyrimidines; RNA; RNA Helicase; Recording of previous events; Regulation; Replicon; Reporting; Role; Signal Transduction; Site; Small Interfering RNA; Structure; System; Therapeutic Intervention; Tissues; Transcription Coactivator; Translation Process; Translations; Up-Regulation; Viral; Viral Genome; Viral Proteins; Virus Replication; c-myc Genes; c-myc Proto-Oncogenes; drug development; helicase; overexpression; protein expression; public health relevance; yeast two hybrid system

DESCRIPTION (provided by applicant): Chronic infection by hepatitis C virus (HCV), which is the leading cause of severe hepatitis, often develops into liver cirrhosis (LC) and hepatocellular carcinoma (HCC). The molecular mechanisms underlying HCV replication and pathogenesis are poorly understood. We have recently identified a cellular factor, FUSE binding protein (FBP) that specifically interacts with HCV 3'NTR and stimulates HCV replication. FBP is known to interact with the pyrimidine-rich far-upstream element (FUSE) of the c-myc proto-oncogene and activate c-myc transcription. C-myc targets approximately 10% of transcribed genes and coordinates many essential cellular processes, including proliferation, growth, and differentiation. C-myc also is consistently elevated in chronically HCV-infected cells, as well as in cells affected by LC and HCC. Our preliminary results have indicated that FBP is overexpressed in HCC with a history of chronic hepatitis C (CHC) but conspicuously absent in other HCC without CHC history. We propose to investigate the mechanisms whereby FPB acts in HCV replication and it possible role in HCV associated pathogenesis. PUBLIC HEALTH RELEVANCE: The major purpose of this proposal is to elucidate the mechanism of FUSE binding protein (FBP)- mediated stimulation of HCV replication and its implication on HC associated pathogenesis. These studies will help delineate how HCV-FBP interactions affect patients infected with HCV, and to determine whether any of these interactions represent targets for therapeutic intervention.

描述（由申请人提供）：严重肝炎的主要原因是丙型肝炎病毒（HCV）的慢性感染，通常会发展为肝肝硬化（LC）和肝细胞癌（HCC）。 HCV复制和发病机理的分子机制知之甚少。我们最近确定了一种细胞因子，融合结合蛋白（FBP），该蛋白（FBP）专门与HCV 3'NTR相互作用并刺激HCV复制。已知FBP与C-MYC原始癌基因的富含嘧啶富胺的远上流元件（FUSE）相互作用并激活C-MYC转录。 C-MYC靶向大约10％的转录基因，并协调许多必需的细胞过程，包括增殖，生长和分化。 C-MYC在慢性HCV感染的细胞以及受LC和HCC影响的细胞中也始终升高。我们的初步结果表明，FBP在HCC中过表达具有慢性肝炎（CHC）史，但在没有CHC病史的其他HCC中明显不存在。我们建议研究FPB在HCV复制中起作用的机制，并在HCV相关的发病机理中可能作用。 公共卫生相关性：该提案的主要目的是阐明融合结合蛋白（FBP）的机制 - 介导的HCV复制刺激及其对HC相关的发病机理的影响。这些研究将有助于描述HCV-FBP相互作用如何影响感染HCV的患者，并确定这些相互作用中的任何一种是否代表了治疗干预的靶标。