La Jolla Interdisciplinary Neuroscience Center Cores - Pro Core BU

拉荷亚跨学科神经科学中心核心 - Pro Core BU

• 批准号: 8132834
• 负责人: STUART A LIPTON
• 金额: $ 10.19万
• 依托单位: SANFORD BURNHAM PREBYS MEDICAL DISCOVERY INSTITUTE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8132834
• 关键词: Amino Acid Sequence; Applications Grants; Arts; Award; Cancer Center; Carbohydrates; Chemicals; Code; Collaborations; Communities; Community Health Centers; Complex; Computer software; Core Facility; Data; Data Analyses; Data Files; Data Quality; Databases; Digestion; Electrospray Ionization; Enzymes; Equipment; Fee-for-Service Plans; Fingerprint; Fostering; Funding; Gel; Glycopeptides; Gold; Grant; Healthcare; Housing; Human Resources; Immunoprecipitation; Individual; Institutes; Intranet; Ions; Isotope Labeling; Isotopes; Maps; Mass Spectrum Analysis; Medical Research; Mission; Neurobiology; Neurosciences; Pathway interactions; Peptide Hydrolases; Peptide Sequence Determination; Peptides; Performance; Pharmaceutical Preparations; Phosphorylation; Post-Translational Protein Processing; Preparation; Process; Protein Analysis; Protein Databases; Proteins; Proteolysis; Proteome; Proteomics; Proteomics Shared Resource; Protocols documentation; Recombinant Proteins; Research Infrastructure; Research Institute; Research Personnel; Resolution; Robotics; SHFM1 gene; Sampling; Scanning; Slice; Solutions; Sorting - Cell Movement; Source; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Spottings; System; Systems Biology; Technology; United States National Institutes of Health; Vendor; Water; authority; base; data format; data management; glycosylation; instrument; instrumentation; knowledge base; mass spectrometer; nano; programs; protein aminoacid sequence; protein complex; research facility; small molecule; synthetic peptide; tandem mass spectrometry

PROTEOMICS CORE 1. OVERVIEW AND MAIN OBJECTIVES The mission of the Neuroscience Proteomics Facility is to provide identification, characterization, and detailed analysis of proteins, peptides, carbohydrates and drug-like small molecules. This facility will allow access to and thereby foster collaborations among Neuroscientists on the La Jolla Torrey Pines Mesa (comprised primarily of Burnham, Salk, Scripps, and UCSD neuroscientists). The facility will leverage the existing infrastructure and knowledge-base of the Burnham Institute for Medical Research's (BIMR's) existing Proteomics Center as well as state-of-the-art mass spectrometry (MS) at The Scripps Research Institute (TSRI) and The Salk Institute, which constitute Satellite Centers located across the street from BIMR on North Torrey Pines Road in La Jolla (and within half a mile from UCSD). Presently this Core Facility at BIMR is part of the Burnham's NIH-funded Cancer Center with little or no access to neuroscientists on the La Jolla Torrey Pines Mesa. Under the present application for an NIH Blueprint Grant for Neuroscience Center Cores, all modern proteomic technologies will be available to neuroscience users of the Facility including comprehensive proteomic analysis of complex proteomes using Multi-dimensional Protein Identification (MUDPIT), activity-based enzyme profiling with chemical mass tags, Product Terminal Isotope Coding for the identification of substrates for proteases (products of proteolysis), phospho-peptide and protein identification using precursor ion scanning, neutral loss scanning and MS3 scanning, and automated high-throughput MALDI-TOF/TOF analysis of protein and peptide mixtures. The Facility also performs the more routine mass analysis of intact recombinant proteins and synthetic peptides and glycopeptides, protein identification from gel slices/spots using peptide mass fingerprinting and peptide sequencing. In the spring of 2006 the Director of the Facility will also apply for funds to purchase an FTMS which will provide the highest mass accuracy and resolution, broad fragmentation capability (such as CID, ECD and IRMPD) and the ability to perform de novo peptide and protein sequencing (top-down proteomics) and directly characterize the most complex post-translational modifications in intact proteins, without prior digestion and processing of the sample. A primary objective for this award is to integrate the proteomics efforts of the La Jolla Neurobiology community into the existing Proteomics Center at the Burnham Institute for Medical Research. This will involve the purchase of additional instrumentation (an LTQ Ion Trap in Year 1), and the hiring of additional personnel. By amalgamating the neuroscience Proteomics Shared Resource with that of the current Center (which includes the Proteomics Facility for the Burnham's Cancer Center as well as the NTCNP Center on Proteolytic Pathways), we will be able to capitalize on the considerable instrumentation and expertise of the existing Center for the benefit of the Neuroscience community. The current grant application will facilitate equal access to these facilities by Neuroscientists, who do not have access currently because the facility is in the Cancer Center. The integration of the Neuroscience proteomics effort into the existing Proteomics Center will provide a substantial jump-start for the Neuroscience proteomics effort. In addition to the current equipment at the Burnham Institute for Medical Research (BIMR) listed above, which is under the direction of Dr. Jeff Smith, satellite facilities are also available at The Salk Institute (under the direction of Wolfgang Fischer) and at The Scripps Research Institute (TSRI), under the direction of noted proteomics authority John Yates, III.

蛋白质组学核心 1.概述和主要成就 神经科学蛋白质组学设施的使命是提供鉴定，表征， 以及对蛋白质、肽、碳水化合物和药物样小分子的详细分析。这个设施 将允许访问，从而促进拉霍亚托里的神经科学家之间的合作 派恩斯梅萨（主要由伯纳姆、索尔克、斯克里普斯和加州大学圣地亚哥分校的神经科学家组成）。该设施 将利用伯纳姆医学研究所现有的基础设施和知识库， 研究（BIMR）现有的蛋白质组学中心以及最先进的质谱（MS）， 斯克里普斯研究所（TSRI）和索尔克研究所，构成卫星中心，位于 在拉霍亚的北托里松树路上，BIMR的街对面（距离UCSD不到半英里）。 目前，BIMR的核心设施是Burnham的NIH资助的癌症中心的一部分， 在拉霍亚托里派恩斯梅萨上接触神经学家在目前申请NIH的情况下， 蓝图拨款神经科学中心核心，所有现代蛋白质组学技术将提供给 该设施的神经科学用户，包括复杂蛋白质组的全面蛋白质组学分析 使用多维蛋白质鉴定（MUDPIT），基于活性的酶谱分析， 质量标记，产品末端同位素编码，用于蛋白酶底物的鉴定（产品 蛋白质水解），磷酸肽和蛋白质鉴定使用前体离子扫描，中性损失 扫描和MS 3扫描，以及蛋白质的自动化高通量MALDI-TOF/TOF分析， 肽混合物。该机构还对完整重组体进行更常规的质量分析 蛋白质和合成肽和糖肽，使用凝胶切片/斑点进行蛋白质鉴定 肽质量指纹分析和肽测序。2006年春季，基金主任将 也申请资金购买FTMS，这将提供最高的质量精度和分辨率， 广泛的裂解能力（如CID、ECD和IRMPD）和从头进行的能力 肽和蛋白质测序（自上而下的蛋白质组学），并直接表征最复杂的 完整蛋白质的翻译后修饰，而无需事先消化和处理样品。 该奖项的主要目的是整合拉霍亚神经生物学的蛋白质组学成果 社区现有的蛋白质组学中心在伯纳姆医学研究所。这将 涉及购买额外的仪器（第1年的LTQ离子阱），并雇用 额外的人员。通过将神经科学蛋白质组学共享资源与 目前的中心（包括伯纳姆癌症中心的蛋白质组学设施以及 NTCNP蛋白水解途径中心），我们将能够利用相当大的 仪器和现有中心的专业知识，为神经科学界的利益。的 目前的拨款申请将促进神经科学家平等地使用这些设施， 目前可以访问，因为该设施位于癌症中心。神经科学的整合 现有的蛋白质组学中心的蛋白质组学工作将提供一个实质性的启动， 神经科学蛋白质组学研究。除了伯纳姆研究所现有的设备外， 上面列出的医学研究（BIMR），由Jeff Smith博士指导， 也可在索尔克研究所（在沃尔夫冈菲舍尔的指导下）和斯克里普斯 研究所（TSRI），在著名的蛋白质组学权威约翰耶茨，三。