Bioavailavility & Bioanalytical Core/Weaver, Connie

核心B的目的是提供生物分析和生物利用度资源的集中来源， 支持个别中心项目。核心B是直接支持项目， 葡萄衍生的多酚级分，并确定葡萄衍生的多酚级分的生物利用度、代谢和分布， 多酚的靶组织，包括大脑。核心B对于中心的执行至关重要 项目和雇用广泛的专业知识，可从普渡大学，伊利诺伊大学和罗格斯大学 与现有的Purdue-UAB植物中心相关。核心B将分为分子 成分和生物分析以及生物利用度和代谢成分，以便表征，存档 并评估葡萄组分的体内生物利用度。分子组成和 生物分析组件将使用 LC/MS方法（Simon和Wu，Rutgers）。通过生物测定对特定多酚级分进行优先级排序 引导分馏，核心将提供LC-MS/MS（Simon和Wu，Rutgers）和NMR方面的专业知识 （Raftery，Purdue）生物活性级分中葡萄衍生多酚的表征以及存档 在项目期间提供服务。（项目1和3）。生物利用度和代谢 核心B的组成部分将使用啮齿动物评估葡萄多酚的吸收、代谢和组织特征 模型，自动血液取样和膜探针由这个小组率先（项目1，2和3， Janle和Weaver，Purdue）。核心还将负责生产（Lila，UIUC）和管理 （Janle，Purdue）的14 C标记的葡萄级分（项目1和3），然后通过以下方法分析体内样品： 加速器质谱（AMS）技术来追踪痕量的葡萄衍生多酚， 它们的代谢产物进入靶组织（项目1、2和3）。血浆药代动力学和 通过LC-MS/MS和LC-NMR完成未标记多酚和代谢物的组织图谱 （项目1、2和3，Ademec、Raftery和Ferruzzi，Purdue）。靶组织中葡萄多酚的分析 新代谢物的鉴定是了解其生物学影响的基础， 老年痴呆症的病理机制。核心B提供的信息将提供关于 葡萄衍生的多酚与阿尔茨海默病风险和结果的调节相关。

The purpose of Core B is to provide a centralized source of Bioanalytical and Bioavailability resources to support individual center projects. Core B is to directly support projects by facilitating characterization of grape derived polyphenol fractions and to determine bioavailability, metabolism and distribution of grapederived polyphenols to target tissues including brain. Core B is critically important for execution of the center projects and employs a wide range of expertise available from Purdue, University of Illinois, and Rutgers associated with the existing Purdue-UAB Botanicals Center. Core B will be divided into Molecular Composition & Bioanalytical and Bioavailability & Metabolism components in order to characterize, archive and assess in vivo bioavailability of grape fractions for individual projects. The Molecular Composition & Bioanalytical Component will provide material authentication and grape polyphenol quantification using the LC/MS methods (Simon and Wu, Rutgers). Following prioritization of specific polyphenol fractions by bioassay guided fractionation, the Core will provide expertise in LC-MS/MS (Simon and Wu, Rutgers) and NMR (Raftery, Purdue) characterization of grape-derived polyphenols in bioactive fractions as well as archiving services for the duration of the project period. (Projects 1 and 3). The Bioavailability & Metabolism Component of Core B will assess grape polyphenol absorption, metabolism and tissue profiles using rodent models, automated blood sampling and membrane probes pioneered by this group (Projects 1,2 and 3, Janle and Weaver, Purdue). The Core will also be responsible for production (Lila, UIUC) and administration (Janle, Purdue) of 14C labeled grape fractions (Projects 1 and 3) followed by analysis of in vivo samples by accelerator mass spectrometry (AMS) techniques to track trace amounts of grape derived polyphenols and their metabolites into target tissues (Project 1,2 and 3). Determination of plasma pharmacokinetics and tissue profiles for unlabeled polyphenols and metabolites will be accomplished by LC-MS/MS and LC-NMR (Projects 1, 2 and 3, Ademec, Raftery and Ferruzzi, Purdue). Analysis of grape polyphenols in target tissues and identification of novel metabolites is fundamental to understanding their biological impact in relation to Alzheimer's pathomechanisms. Information provided by Core B will provide critical knowledge on the fate of grape derived polypohenols associated with modulation of Alzheimer's disease risk and outcomes.