Role of Extracellular Matrix in Retinal Development and Disease

细胞外基质在视网膜发育和疾病中的作用

• 批准号: 7992716
• 负责人: WILLIAM J BRUNKEN
• 金额: $ 39.75万
• 依托单位: SUNY DOWNSTATE MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-08-01 至 2013-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7992716
• 关键词: Address; Adherens Junction; Adhesions; Affect; Amacrine Cells; American; Animals; Apical; Architecture; Basement membrane; Blindness; Blood Vessels; Brain; Cell Adhesion; Cell Cycle; Cell Polarity; Cell Proliferation; Cell physiology; Cells; Cicatrix; Collagen; Complex; Congenital Abnormality; Data; Defect; Dependence; Deposition; Development; Disease; Dystroglycan; Extracellular Matrix; Eye diseases; Functional disorder; Genes; Glaucoma; Gliosis; Goals; Head; Health; Homeostasis; Inner Limiting Membrane; Inorganic Sulfates; Integrins; Ion Channel; Knockout Mice; Laminin; Lateral; Lead; Left; Literature; Mediating; Mental Retardation; Molecular; Muller' s cell; Mus; Muscular Dystrophies; Neural Retina; Pathology; Photoreceptors; Potassium Channel; Process; Proliferative Vitreoretinopathy; Publishing; Regulation; Retina; Retinal; Retinal Defect; Retinal Ganglion Cells; Role; Secondary to; Series; Site; Structure-Activity Relationship; Surface; Synapses; Syndrome; Testing; Therapeutic; Trauma; Unspecified or Sulfate Ion Sulfates; Vitreous body structure; Walkers; Work; design; in vivo; malformation; migration; novel diagnostics; null mutation; public health relevance; receptor; research study; scaffold; synaptogenesis; water channel

DESCRIPTION (provided by applicant): The Muller cells (MCs) have a key role in retinal homeostasis. They are polarized with an apical compartment facing the subretinal space, and a basal surface adhering to the inner limiting membrane (ILM). Disruption of either compartment leads to activation of MCs and pathobiological consequences leading to vision loss. Laminins organize basement membranes (BM), such as the ILM serving as attachment sites for cell adhesion, polarity and proliferation. We produced mice with null mutations in two laminin genes, Lamb2 and Lamc3, and found that these molecules are necessary for normal retinal development. The defects in the Lamb2/c3 nulls include: ILM malformation; MC disorganization; photoreceptor dysgenesis; and progressive pathology in the inner retina. Our long-range goal is to understand the molecular contributions of laminin to the development and stability of MC organization, in particular, as it relates to the functional architecture. Our immediate goal is to assess the organizing hypothesis that MC-laminin interactions are critical for: 1) establishing MC polarity; 2) maintaining MC sub cellular organization and 3) stabilizing the structural integrity of the retina. In Aim 1, we will examine the molecular mechanisms underlying laminin- mediated scaffolding in the MC. We will answer the following three questions. First, we will address: What Is The Molecular Organization Of The Adhesion Complex Of The MC Basal Endfoot? Next, we will determine: What Is The Laminin Dependence Of Ion Channel Sub cellular Localization And Function? Finally, we will investigate: What Is The Mechanism Of Laminin Regulation Of MC Proliferation? In Aim 2, we will examine the corollary hypothesis that the loss of adhesion to laminin substrates disrupts the normal structure/function relationships in the inner retina. Our data show that the Lamb2/c3 null animals have selective disruptions in the IPL. We will answer the following questions. First, we will ask: What Is The Laminin Dependence Of Dendritogenesis In The IPL? Next, we will address: What Are The Mechanisms Of Progressive Retinal Ganglion Cell Loss in Lamb2/Lamc3 nulls? These experiments and the molecular mechanisms they reveal will contribute to our understanding of the pathophysiology of gliosis; proliferative vitreoretinopathy and congenital ocular disorders including Walker-Warburg, Bardet-Biedl, Pierson Syndrome. PUBLIC HEALTH RELEVANCE: Congenital birth defects affect millions of Americans and people world-wide. These diseases include muscular dystrophy, brain and eye disorders leading to mental retardation and blindness. This project investigates the cause of several of these diseases and the results will lead to new diagnostics and therapeutics.

描述（由申请人提供）：Muller细胞（MC）在视网膜稳态中具有关键作用。它们被极化，顶室面向视网膜下腔，基底表面粘附在内界膜（ILM）上。任何一个区室的破坏都会导致MC的激活和导致视力丧失的病理生物学后果。层粘连蛋白组织基底膜（BM），例如作为细胞粘附、极性和增殖的附着位点的ILM。我们产生了两个层粘连蛋白基因Lamb 2和Lamc 3无效突变的小鼠，并发现这些分子是正常视网膜发育所必需的。Lamb 2/c3无效的缺陷包括：ILM畸形; MC解体;感光细胞发育不全;以及内层视网膜中的进行性病理。我们的长期目标是了解层粘连蛋白的分子贡献MC组织的发展和稳定性，特别是，因为它涉及到的功能架构。我们的近期目标是评估MC-层粘连蛋白相互作用对于以下方面至关重要的组织假说：1）建立MC极性; 2）维持MC亚细胞组织和3）稳定视网膜的结构完整性。 在目标1中，我们将研究MC中层粘连蛋白介导的支架形成的分子机制.我们将回答以下三个问题。首先，我们将解决：什么是MC基底端的粘附复合物的分子组织？下一步，我们将确定：什么是离子通道亚细胞定位和功能的层粘连蛋白依赖性？最后，我们将探讨：层粘连蛋白调节MC增殖的机制是什么？ 在目标2中，我们将研究的推论假设，即层粘连蛋白基板的损失破坏了正常的结构/功能关系的内层视网膜。我们的数据显示，Lamb 2/c3缺失动物在IPL中具有选择性破坏。我们将回答以下问题。首先，我们会问：什么是层粘连蛋白依赖的树突状细胞在强脉冲光？下一步，我们将解决：Lamb 2/Lamc 3无效的进行性视网膜神经节细胞丢失的机制是什么？ 这些实验及其揭示的分子机制将有助于我们理解神经胶质增生、增生性玻璃体视网膜病变和先天性眼部疾病（包括Walker-Warburg、Bardet-Biedl、Pierson综合征）的病理生理学。 公共卫生相关性：先天性出生缺陷影响着数百万美国人和世界各地的人们。这些疾病包括肌肉萎缩症、导致智力迟钝和失明的脑部和眼部疾病。该项目调查了其中几种疾病的原因，其结果将导致新的诊断和治疗方法。