STRUCTURAL CHARACTERIZATION OF PROTEINS INVOLVED IN HOST/PATHOGEN INTERACTIONS

参与宿主/病原体相互作用的蛋白质的结构特征

• 批准号: 8170078
• 负责人: Martin J Boulanger
• 金额: $ 0.13万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-01 至 2011-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8170078
• 关键词: Adult; Antigens; Aromatic Compounds; Autacoids; Bacteria; Bacterial Adhesins; Binding; Bioremediations; Cells; Computer Retrieval of Information on Scientific Projects Database; Engineering; Environment; Environmental Pollutants; Enzymes; Excision; Funding; Grant; Infection; Institution; Invaded; Mediating; Membrane Proteins; Metabolic Pathway; Parasites; Petroleum; Polychlorinated Biphenyls; Population; Property; Proteins; Research; Research Personnel; Resources; Site; Solutions; Source; Therapeutic Intervention; Toxoplasma gondii; United States National Institutes of Health; Vaccines; Work; Xenobiotics; base; biological systems; design; member; novel; pathogen; programs; prophylactic; remediation

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Project #1 - The protozoan parasite Toxoplasma gondii is a serious global pathogen that infects nearly one third of the adult human population. Despite its importance, very little is known about how this parasite initially engages a host cell in order to establish infection. The aim of this proposal is to characterize the molecular interactions that enable T. gondii to attach to, and ultimately invade virtually every nucleated cell. More specifically, we will characterize the structural basis of how key members of a superfamily of developmentally expressed surface proteins on T. gondii (known as the SRS adhesins/antigens) mediate parasite attachment to host cells. Based on this work, we will be strategically poised to develop therapeutic interventions, either prophylactic or vaccine-based, to limit infectivity of this widespread zoonotic pathogen. Project #2 - Xenobiotic aromatic compounds represent one of the most significant classes of environmental pollutants. There is an ever growing need for inexpensive, biologically based solutions to deal with these types of aromatic molecules such as those found in crude oil, PCBs or industrial effluents. Remediation of these contaminated sites is both technically challenging and expensive. A promising strategy to stimulate the removal of these contaminants from the environment is to manipulate the metabolic pathways of bacteria. As a prerequisite step to the rational engineering of these biological systems, detailed descriptions of the enzymes involved are essential. Towards this end, my research program is focused on characterizing the structural properties of novel aromatic catabolic enzymes from a potent PCB-degrading bacterium. The long-term objectives of this research program are to design more efficient strategies for the bioremediation of environmental pollutants.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 项目＃1-原生动物寄生虫弓形虫Gondii是一种严重的全球病原体，可感染近三分之一的成年人人口。尽管它很重要，但对于该寄生虫最初如何使宿主细胞与建立感染相关的知识知之甚少。该提议的目的是表征使T. gondii能够附着并最终侵入几乎所有核定细胞的分子相互作用。更具体地说，我们将表征gondii（称为SRS粘附素/抗原）上发育表达的表面蛋白的主要成员如何介导寄生虫附着在宿主细胞上的结构基础。基于这项工作，我们将在战略上准备开发预防性或基于疫苗的治疗性干预措施，以限制这种广泛的人畜共患病原体的感染性。 项目＃2-异种生物芳香化合物代表了最重要的环境污染物之一。越来越需要廉价，基于生物学的解决方案来处理这些类型的芳香族分子，例如原油，PCB或工业废水中发现的分子。对这些受污染的地点的修复既具有挑战性又昂贵。刺激这些污染物从环境中去除的有前途的策略是操纵细菌的代谢途径。作为这些生物系统合理工程的前提步骤，对所涉及的酶的详细描述至关重要。为此，我的研究计划的重点是表征有效的PCB降解细菌的新型芳族分解代谢酶的结构特性。该研究计划的长期目标是为环境污染物的生物修复设计更有效的策略。