STRUCTURAL STUDIES OF THE PHOTOSYNTHETIC REACTION CENTER FROM RHODOBACTER SPHAER

红细菌SPHAER光合反应中心的结构研究

• 批准号: 8169989
• 负责人: MELVIN Y OKAMURA
• 金额: $ 0.07万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-01 至 2011-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8169989
• 关键词: Affect; Bacteriochlorophylls; Binding; Charge; Chemicals; Complex; Computer Retrieval of Information on Scientific Projects Database; Cytochromes c2; Electron Transport; Energy Transfer; Event; Fibrin fragment D; Funding; Grant; Harvest; Institution; Integral Membrane Protein; Light; Photosynthetic Reaction Centers; Pigments; Proteins; Protons; Quinones; Reaction; Research; Research Personnel; Resources; Rhodobacter; Source; Structure; Sunlight; United States National Institutes of Health; Water; Work; mutant

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Reaction Center (RC) is the integral membrane protein that performs the light induced electron transfer reactions that are the first steps in conversion of sunlight into chemical energy. Electron transfer occurs from the excited state of a bacteriochlorophyll dimer (D) through several intermediate acceptor molecules to two quinone molecules, QA and QB. The RC forms a complex with another pigment-containing integral membrane protein called the light-harvesting complex (LH1) whose primary function is to gather light and transfer this energy to the RC and photo-oxidize D. In addition, a 14-kilodalton water soluble-cytochrome c2 transiently binds to the RC and re-reduces D. Our ongoing work at SSRL will focus on three structure-function aspects of the RC: 1). Determination of the structure of the RC complexed with other proteins: a). RC and light harvesting protein (LH1), and b). Mutants that affect the binding and electron transfer between RC with cyt c2. 2). Determination of the structures of mutants that affect proton and electron transfer events in RC. 3). Determination of the structures of intermediates that are involved in the charge separation.

这个子项目是许多利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 反应中心（RC）是膜蛋白的一部分，它负责光诱导的电子转移反应，这是将阳光转化为化学能的第一步。电子从细菌叶绿素二聚体（D）的激发态通过几个中间受体分子转移到两个醌分子QA和QB。RC与另一种含有色素的膜蛋白形成复合物，称为捕光复合物（LH 1），其主要功能是收集光并将此能量转移到RC并光氧化D。此外，14千道尔顿的水溶性细胞色素c2瞬时结合RC并重新还原D。我们在SSRL正在进行的工作将集中在RC的三个结构-功能方面：1）。确定与其他蛋白质复合的RC的结构：a）. RC和捕光蛋白（LH 1），和B）。影响RC与cyt c2之间结合和电子转移的突变体。2）。确定影响RC中质子和电子转移事件的突变体的结构。（3）第三章。确定参与电荷分离的中间体的结构。