X-RAY CRYSTALLOGRAPHY OF IMMUNE-RELATED MOLECULES

免疫相关分子的 X 射线晶体学

• 批准号: 8170089
• 负责人: IAN A WILSON
• 金额: $ 0.66万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-01 至 2011-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8170089
• 关键词: Amyloidosis; Antibodies; CD1 Antigens; Complex; Computer Retrieval of Information on Scientific Projects Database; Crystallography; Drug Design; Enzymes; Evolution; Funding; Glycoproteins; Grant; HIV Envelope Protein gp120; HIV Envelope Protein gp41; HIV-1; Hemagglutinin; Human; Immune; Immune system; Immunologic Receptors; Influenza; Institution; Laboratories; Lampreys; Malignant Neoplasms; Neuraminidase; Phosphoribosylglycinamide formyltransferase; Prealbumin; Proteins; Research; Research Personnel; Resources; Shark; Source; Structure; T-Cell Receptor; T-Lymphocyte; Toll-like receptors; United States National Institutes of Health; Viral Antigens; Virus; Work; base; influenzavirus; inhibitor/antagonist; interest; neutralizing antibody; phosphoribosylaminoimidazolecarboxamide formyltransferase; programs; synchrotron radiation

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Our laboratory carries out structural studies of molecules involved in our adaptive and innate immune systems. These molecules include antibodies, T cell receptors, major histocompatibility complex molecules, CD1 molecules and Toll-like receptors, as well as their cellular co-factors. We have an extensive program studying neutralizing antibodies against the HIV-1 virus and the influenza virus, and will be studying complexes of these antibodies with their viral antigens gp120 and gp41 (from HIV-1) and hemagglutinin and neuraminidase (from influenza). In addition, we are interested in the evolution of the immune system and are studying immune receptors from shark and lamprey in order to see how they compare to their mammalian counterparts. Many of the proteins we are studying are glycoproteins that tend to diffract weakly, so the use of synchrotron radiation is critical for their structure determinations. In addition to the immune-system related work, we are studying the enzymes GAR transformylase and AICAR transformylase, in complex with inhibitors, in a program of anti-cancer structure-based drug design and also human transthyretin in complex with anti-amyloidosis inhibitors.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 我们的实验室对参与我们的适应性和先天免疫系统的分子进行结构研究。这些分子包括抗体、T细胞受体、主要组织相容性复合物分子、CD 1分子和Toll样受体，以及它们的细胞辅因子。我们有一个广泛的项目，研究针对HIV-1病毒和流感病毒的中和抗体，并将研究这些抗体与其病毒抗原gp 120和gp 41（来自HIV-1）以及血凝素和神经氨酸酶（来自流感）的复合物。此外，我们对免疫系统的进化感兴趣，并正在研究鲨鱼和七鳃鳗的免疫受体，以了解它们与哺乳动物的免疫受体相比如何。我们正在研究的许多蛋白质都是糖蛋白，它们的构象很弱，因此使用同步辐射对它们的结构测定至关重要。除了免疫系统相关的工作，我们正在研究酶GAR transformylase和AICAR transformylase，与抑制剂的复合物，在抗癌结构为基础的药物设计的程序，也与抗淀粉样变性抑制剂的复合物人甲状腺素运载蛋白。