SYNTHESIS AND MASS SPECTROMETRY ANALYSIS OF NUCLEOSIDE ANALOGS

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 麦克劳克林小组的研究涉及三个相关领域：(1)他们制备核苷类似物作为潜在的抗病毒药物。他们研究了碱基对和糖的结构变化。他们的目标是开发具有不同聚合酶活性的化合物--对病毒有活性，对人类聚合酶没有活性--这将有助于开发新的抗病毒药物。(2)理解大分子识别过程是理解基因表达的关键。为了探索这种过程，他们准备了修饰的DNA序列，其中识别位点发生了功能性改变。他们使用这些改变的DNA序列来探测结构、功能和识别。(3)DNA的自然结构特征有助于构建由互补DNA序列杂交形成的重复晶格。这些纳米结构可用作多组分体系的结晶支架，隔离纳米级溶质或控制纳米级药物的释放。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The McLaughlin group's research spans three related areas: (1) they prepare nucleoside analogues as potential antivirals. They examine structural alterations on both the nucleobase as well as the sugar. Their goal is to develop compounds that exhibit differential activity with polymerase - active with the virus, inactive wit human polymerases - this will help to develop new anti-virals. (2) Understanding macromolecular recognition processes is critical to understanding gene expression. To probe such processes, they prepare modified DNA sequences in which the recognition sites are functionally altered. They use these altered DNA sequences to probe structure, function and recognition. (3) The natural structural characteristics of DNA lend themselves to the construction of repeating lattices formed by hybridization of complementary DNA sequences. These nanoscale structures can be used as crystallization scaffolds for multi-component systems, to sequester nanometer sized solutes or for controlled release of nanoscale pharmaceuticals.