THE IFS-SPN INTERFACE

IFS-SPN 接口

• 批准号: 8168725
• 负责人: TOM E. ELLENBERGER
• 金额: $ 0.43万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-03-10 至 2010-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8168725
• 关键词: Binding; Codon Nucleotides; Complex; Computer Retrieval of Information on Scientific Projects Database; Data; Disease; Eating; Funding; Goals; Gram-Positive Bacteria; Grant; Immunity; Institution; Names; Pharyngeal structure; Proteins; Protocols documentation; Research; Research Personnel; Resolution; Resources; Source; Streptococcus pyogenes; Structure; Toxin; United States National Institutes of Health; Universities; Washington; inhibitor/antagonist; novel

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The teams of Caparon and Hultgren at Washington University in St. Louis recently identified the protein toxin (SPN, ~36 kDa) secreted by Streptococcus pyogenes, a Gram-positive bacteria responsible for causing strep throat and flesh eating disease. A novel endogenous inhibitor, named Immunity Factor for SPN (IFS, ~ 22kDa) was recently discovered on the same codon as SPN. IFS forms a stable complex with SPN at a 1:1 molar ratio and inhibits SPN's activity by acting as a competitive inhibitor. The crystal structure of IFS was determined in the Ellenberger group at Washington University in St. Louis (unpublished data). Our goal is to use the high resolution PLIMSTEX protocol to locate the binding interface of IFS to SPN. The crystal structure of the complex is underway.

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 圣路易斯华盛顿大学的Caparon和Hultgren团队最近发现了化脓性链球菌分泌的蛋白质毒素(SPN，~36 kDa)。化脓性链球菌是一种革兰氏阳性细菌，可导致链球菌咽喉和食肉疾病。最近在与SPN相同的密码子上发现了一种新的内源性抑制因子，命名为SPN免疫因子(IfS，~22 kDa)。IFs与SPN以1：1的摩尔比形成稳定的络合物，并通过竞争性抑制作用抑制SPN的活性。在圣路易斯华盛顿大学的埃伦伯格小组中确定了IF的晶体结构(未发表的数据)。我们的目标是使用高分辨率的PLIMSTEX协议来定位IFS与SPN的绑定接口。该复合体的晶体结构正在研究中。