THE IFS-SPN INTERFACE

IFS-SPN 接口

• 批准号: 8361370
• 负责人: TOM E. ELLENBERGER
• 金额: $ 2.16万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-01-01 至 2011-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8361370
• 关键词: Binding; Codon Nucleotides; Complex; Data; Disease; Eating; Funding; Goals; Gram-Positive Bacteria; Grant; Immunity; Mass Spectrum Analysis; Names; National Center for Research Resources; Pharyngeal structure; Principal Investigator; Proteins; Protocols documentation; Publishing; Research; Research Infrastructure; Resolution; Resources; Source; Streptococcus pyogenes; Structure; Toxin; United States National Institutes of Health; Universities; Washington; Work; biomedical resource; cost; inhibitor/antagonist; novel

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The teams of Caparon and Hultgren at Washington University in St. Louis recently identified the protein toxin (SPN, ~36 kDa) secreted by Streptococcus pyogenes, a Gram-positive bacteria responsible for causing strep throat and flesh eating disease. A novel endogenous inhibitor, named Immunity Factor for SPN (IFS, ~ 22kDa) was recently discovered on the same codon as SPN. IFS forms a stable complex with SPN at a 1:1 molar ratio and inhibits SPN's activity by acting as a competitive inhibitor. The crystal structure of IFS was determined in the Ellenberger group at Washington University in St. Louis (unpublished data). Our goal is to use the high resolution PLIMSTEX protocol to locate the binding interface of IFS to SPN. The crystal structure of the complex is underway. We are planning to publish this work in the coming year.

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其他NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 位于圣路易斯的华盛顿大学的Caparon和Hultgren的研究小组最近发现了由化脓性链球菌分泌的蛋白质毒素（SPN，约36 kDa），化脓性链球菌是一种革兰氏阳性细菌，可引起链球菌性咽喉炎和食肉疾病。 最近发现一种新的内源性抑制因子，命名为SPN免疫因子（Immunity Factor for SPN，IFS，~ 22 kDa）。 IFS与SPN以1：1的摩尔比形成稳定的复合物，并通过充当竞争性抑制剂来抑制SPN的活性。 IFS的晶体结构由圣路易斯华盛顿大学的Ellenberger小组确定（未发表的数据）。 我们的目标是使用高分辨率的PLIMSTEX协议来定位IFS到SPN的绑定接口。 复杂的晶体结构正在进行中。 我们计划在明年出版这项工作。