DELETION OF UDP-GLUCOSE PYROPHOSPHORYLASE REVEALS A UDP

UDP-葡萄糖焦磷酸化酶的缺失揭示了 UDP

• 批准号: 8168824
• 负责人: ANNE-CHRISTIN LAMERZ
• 金额: $ 1.15万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-03-10 至 2010-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8168824
• 关键词: Anabolism; Computer Retrieval of Information on Scientific Projects Database; Environment; Funding; Galactose; Glucose; Glycocalyx; Glycoconjugates; Grant; Hexoses; Human; Institution; Leishmania; Leishmania major; Parasites; Pathway interactions; Research; Research Personnel; Resources; Source; Surface; UTP-Glucose-1-Phosphate Uridylyltransferase; United States National Institutes of Health; Uridine Diphosphate Galactose; Uridine Diphosphate Glucose; epimerization; galactokinase; glucose 1 phosphate; inorganic phosphate; sugar nucleotide

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The nucleotide sugar UDP-galactose is essential for the biosynthesis of the main surface glycoconjugates of the human parasite Leishmania major. It is synthesized de novo by epimerization of UDP-glucose and by an undefined salvage pathway. Activation of galactose taken up from the environment typically involves a galactokinase and UDP-glucose-hexose-1-phosphate uridylyltransferase according to the Leloir pathway. Since both the de novo and Leloir pathways requires UDP-Glucose, the UDP-glucose pyrophosphorylase (UGP) catalyzing activation of glucose-1 phosphate to UDP-glucose was expected to control Leishmania glycocalyx formation.

这个子项目是许多利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 核苷酸糖UDP-半乳糖是人体寄生虫利什曼原虫主要表面糖缀合物生物合成所必需的。通过UDP-葡萄糖的差向异构化和未定义的补救途径从头合成。根据Leloir途径，从环境中摄取的半乳糖的活化通常涉及半乳糖激酶和UDP-葡萄糖-己糖-1-磷酸尿苷酰转移酶。 由于从头和Leloir途径都需要UDP-葡萄糖，因此预期UDP-葡萄糖焦磷酸化酶（UGP）催化葡萄糖-1磷酸活化为UDP-葡萄糖可控制利什曼原虫糖萼形成。