Chromatin Remodeling and Transcription Repression

染色质重塑和转录抑制

• 批准号: 7995660
• 负责人: Blaine Bartholomew
• 金额: $ 14.13万
• 依托单位: SOUTHERN ILLINOIS UNIVERSITY CARBONDALE
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-02-12 至 2010-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7995660
• 关键词: ATPase Domain; Architecture; Back; Biochemical; Biological Process; Catalytic Domain; Cells; Chromatin; Chromatin Remodeling Factor; Chromosomes; Code; Complex; DNA; DNA Packaging; Deletion Mutagenesis; Deletion Mutation; Development; Gene Expression; Genes; Genetic Techniques; Genomics; Goals; Health; Histones; ISWI; ISWI Family Protein; In Vitro; Mediating; Methods; Molecular; Molecular Genetics; Nucleosomes; Process; Property; Proteins; RNA Polymerase II; RNA chemical synthesis; Reading; Regulation; Repression; Role; Series; Site; Small Interfering RNA; Staging; Stem cells; Structure; Techniques; Tertiary Protein Structure; Transcription Elongation; Untranslated RNA; cell type; cellular development; chromatin remodeling; crosslink; domain mapping; enzyme activity; gene repression; genetic analysis; histone modification; in vivo; macromolecular assembly; mutant; novel; protein protein interaction; repaired; tumorigenesis; yeast genetics

Project Summary: The ISWI type chromatin remodeling complexes have been shown to be involved in transcription repression, and the regulation of transcription elongation and termination. Reorganization of chromatin and regions in chromosomes are fundamental in cellular development and differentiation. We propose to further our understanding of these processes by a two-prong approach of (1) extensive biochemical characterization of the ISWI family of proteins and (2) the molecular genetic analysis of mutant versus wild type ISWI complexes in vivo.

项目摘要： ISWI型染色质重塑复合物已被证明参与了 转录抑制，以及转录延长和终止的调节。 染色质和染色体区域的重组是细胞遗传学的基础。 发展和分化。我们建议进一步了解这些 通过双管齐下的方法（1）广泛的生物化学表征的过程 ISWI家族蛋白质和（2）突变体与野生型的分子遗传学分析 体内ISWI复合物。