More Complete Assessment of DNA Variation in Age-Related Macular Degeneration

描述(由申请人提供)：这项提案建立在密歇根大学和宾夕法尼亚大学科学家之间积极和富有成效的合作基础上。研究小组对我们理解黄斑变性的遗传学以及可用于黄斑变性和其他疾病的基因组研究的一系列统计方法和分析工具做出了几项贡献。老年性黄斑变性(AMD)是一种进行性神经退行性疾病，是老年人致盲的主要原因。黄斑变性导致的视力丧失目前是不可逆转的。在过去的几年里，通过SNP基因分型研究，我们在理解导致黄斑变性的分子机制方面取得了很大进展，这些研究集中在一类容易获得的常见DNA序列变体上。在这里，我们建议利用DNA测序和基因分型技术的进展来更系统地评估DNA序列变异在老年性黄斑变性易感性中的作用。我们的研究团队不仅包括黄斑变性的临床专业知识和理解，还包括高通量遗传学和基因组学以及尖端统计和计算方法的开发和应用方面的专业知识。通过深度外显子组重测序和低通全基因组测序相结合的方法，我们建议描述3000个个体的遗传变异，并评估&gt；20M个遗传变异对疾病易感性的贡献，不仅包括常见的SNPs，还包括罕见的SNPs、短插入缺失多态和较大拷贝数的变异。我们的方法应该会产生新的黄斑变性易感基因，并提高我们对先前涉及的基因座上导致疾病易感性的分子机制的理解。 公共卫生相关性：老年性黄斑变性(AMD)是一种进行性神经退行性疾病，是老年人失明的主要原因。黄斑变性导致的视力丧失目前是不可逆转的。以往对老年性黄斑变性的遗传学研究主要集中在一类容易获得的DNA序列变体上。我们已经组建了一个专家团队，研究年龄相关性黄斑变性的临床特征，分析DNA序列变异的方法和工具，以及相关的计算问题，并建议更彻底地评估DNA序列变异对疾病易感性的贡献。我们希望我们的研究将导致新的疾病易感性变异，并更好地理解导致疾病的分子变化。这些知识将有助于开发新的治疗方法和制定早期诊断和预防疾病的战略。

DESCRIPTION (provided by applicant): This proposal builds on active and productive collaboration between scientists at the University of Michigan and at the University of Pennsylvania. The research team has made several contributions both to our understanding of the genetics of macular degeneration and to the array of statistical methods and analytical tools available for genomic studies of macular degeneration and other disorders. Age-related macular degeneration (AMD) is a progressive neurodegenerative disease and the major cause of blindness among the elderly. Loss of vision caused by macular degeneration is currently irreversible. In the past several years, great progress has been made in our understanding of the molecular mechanisms that lead to macular degeneration through SNP genotyping studies, which focus on an easily accessible class of common DNA sequence variants. Here, we propose to use advances in DNA sequencing and genotyping technology to more systematically evaluate the role of DNA sequence variation in susceptibility to age related macular degeneration. Our research team includes not only clinical expertise and understanding of macular degeneration but also expertise in high-throughput genetics and genomics and in the development and application of cutting edge statistical and computational methods. Through a combination of deep exome resequencing and low pass whole genome sequencing we propose to characterize genetic variation in 3,000 individuals and evaluate the contribution of >20M genetic variants to disease susceptibility, including not only common SNPs, but also rare SNPs, short insertion deletion polymorphisms, and larger copy number variants. Our approach should yield new susceptibility loci for macular degeneration and improve our understanding of the molecular mechanisms that contribute to disease susceptibility in previously implicated loci. PUBLIC HEALTH RELEVANCE: Age-related macular degeneration (AMD) is a progressive neurodegenerative disease and the major cause of blindness among the elderly. Loss of vision caused by macular degeneration is currently irreversible. Previous genetic studies of age-related macular degeneration focused on a class of easily accessible DNA sequence variants. We have assembled a team of experts in the clinical features of age related macular degeneration, in the methods and tools for the analysis of DNA sequence variation, and in associated computational problems and propose to more thoroughly assess the contribution of DNA sequence variation to disease susceptibility. We expect our research will lead to new disease susceptibility variants and better understanding of the molecular changes that lead to disease. This knowledge will facilitate development of new therapies and development of strategies for early diagnosis and prevention of disease.