Modeling of S.Aureus Transmission in Northern Manhattan

说明(由调查员提供)：本修订版是对NOT-OD-09-058号通知的回应，该通知的标题为：“使RPG能够利用NCRR中心和类似中心的计划”。本次修订的目的是利用我们正在进行的R01的现有框架，标题为：“金黄色葡萄球菌在曼哈顿北部的传播”。总体目标是确定ST398菌株作为一种新出现的病原体在我们社区的重要性，并确定其毒力的基础。我们的新目标如下所示。A.ST398在曼哈顿北部的宿主和传播方式是什么？一项基于集群的研究设计将用于确定ST398在曼哈顿北部传播的基础。被定植或感染ST398的受试者将被识别、访谈和培养。所有联系人及其次要联系人都将被识别并被要求参与。将确定与该菌株的获取和传播相关的因素。将努力招募所有网络成员，包括最近在多米尼加共和国的联系人。根据ST398过去动物起源的历史，我们将检查社区中潜在的动物储存库，包括宠物商店、兽医、屠夫和宠物美容师。还将使用确定菌株的位置的空间地图来确定潜在的传播模式或传播的宿主。哪些基因改变是导致ST398菌株适应人际传播的原因？我们将使用全基因组测序将曼哈顿北部的ST398分离株与那些与动物相关感染直接相关的分离株进行比较。我们将确定ST398基因组中独特的区域，并可能解释这些克隆的传播性和毒力增加的原因。将开发一种分子信标分析来更容易地识别ST398。这将允许在大量金黄色葡萄球菌分离株中更有效和更快速地筛选ST398。这项研究将利用哥伦比亚大学欧文临床和翻译研究中心的资源来帮助开展这项以社区为基础的调查。此次修订将提供两个新的职位，并为协助AIM 2的全基因组测序公司提供额外的工作。 公共卫生相关性：本修订附录的目标是确定金黄色葡萄球菌菌株ST398的重要性，这种菌株最初与动物工作者的感染有关，现在在曼哈顿北部流行，作为我们社区的一种新病原体，并确定其毒力的基础。

DESCRIPTION (provided by investigator): This revised supplement is responsive to the notice NOT-OD-09-058 entitled: "Enabling RPGs to Leverage NCRR Center and Center-like Programs". The aims of this revision are designed to utilize the existing framework of our ongoing R01 entitled: "S. aureus transmission in Northern Manhattan." The overall goal is to define the importance of strain ST398 as an emerging pathogen in our community and to determine the basis for its virulence. Our new aims are listed below. a. What are the reservoirs and modes of transmission of ST398 in Northern Manhattan? A cluster-based study design will be used to define the basis for ST398 transmission in Northern Manhattan. Subjects either colonized or infected with ST398 will be identified, interviewed, cultured. All contacts and their secondary contacts will be identified and asked to participate. Factors associated with acquisition and transmission of this strain will be determined. Efforts will be made to enroll all network members including recent contacts in the Dominican Republic. In light of the past history of an animal origin for ST398 we will examine potential animal reservoirs in the community including pet stores, veterinarians, butchers and pet groomers. Spatial mapping of the location the strains were identified will also be used to identify potential patterns of or reservoirs for transmission. b. What genetic alterations are responsible for the adaptation of strain ST398 to person-to-person transmission? We will use total genome sequencing to compare the Northern Manhattan ST398 isolates with those isolates that have been directly associated with animal related infections. We will identify regions of the ST398 genome that are unique and might explain the increased transmissibility and virulence of these clones. A molecular beacon assay will be developed to more easily identify ST398. This will allow more efficient and rapid screening for ST398 in large collections of S. aureus isolates. The study will utilize the resources of the Columbia University Irving Center for Clinical and Translational Research to help carry out this community-based investigation. The revision will provide two new positions as well as provide additional work for the total genome sequencing company assisting with aim 2. PUBLIC HEALTH RELEVANCE: The goal of this revised supplement is to define the importance of S. aureus strain ST398, a strain originally associated with infections among animal workers now prevalent in Northern Manhattan, as an emerging pathogen in our community and to determine the basis for its virulence.