Virology and Technology Core

病毒学和技术核心的目的有两个：1)提供设施和技术 协助在小鼠中进行病毒感染和检测，以及2)促进使用新的 我们通过每个项目开发的检测针对同种异体抗原的免疫反应的方法。 生物安全二级/三级(BSL2/3)生物遏制套房内的一个房间将被分配用于 病毒，用于随后的监测和监测，以及小鼠的出血和收获 纸巾。对于病毒和免疫学分析，我有一个BSL2实验室区域，它有一个单独的附加 组培室配有培养箱和层流罩，用于组织培养工作。这一核心将 负责病毒的生长、制备、纯化和检测，保持多肽的库存 用于抗原特异性MHC-多聚体试剂(MHC-LG-二聚体和MHC四聚体)的制备。 核心中心将为量化和表征病毒特异性的化验提供试剂和培训 T细胞，包括MHC多聚体的使用，T细胞增殖分析，细胞内细胞因子分析，以及 抗病毒CTL检测。将使用的新技术包括体内细胞毒性试验，以测定NK和 T细胞对同种异体组织的活性，以及细胞内细胞因子检测幼稚， 效应器/记忆，以及耐受的同种异体反应T细胞。将由核心提供的服务将可用 对所有项目实验室，核心还将负责培训人员进行周遭分析

The purpose ofthe Virology and Technology Core is two fold: 1) to provide facilities and technical assistance for the conduction of virus infections and assays in mice, and 2) to facilitate the use of novel assays that we have developed by each ofthe Projects to examine immune responses against alloantigens. One room within a Biosafety level two/three (BSL2/3) Biocontainment Suite will be assigned for work with viruses, for their subsequent surveillance and monitoring, and for the bleeding of mice and harvesting of tissues. For viral and immunological assays, I have a BSL2 laboratory area that has a separate attached tissue culture room equipped with incubators and laminar flow hoods for tissue culture work. This Core will be responsible for the growth, preparation, purification, and assay of viruses, maintaining stocks of peptide epitopes, and for the preparation of antigen-specific MHC-multimer reagents (MHC-lg-dimers and MHCtetramers). The Core will provide reagents and training for assays to quantify and characterize virus-specific T cells including the use of MHC-multimers, T cell proliferation assays, intracellular cytokine assays, and antiviral CTL assays. The new technology to be used include in vivo cytotoxicity assays to measure NK and T cell activity against allogeneic tissues, and intracellular cytokine assays to examine naive, effector/memory, and tolerized alloreactive T cells. The services to be provided by the Core will be available to all Project Laboratories, and the Core will also be responsible for training personnel to peri'orm assays