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Mouse and Transplantation Core

小鼠和移植核心

基本信息

批准号：
7994929
负责人：
DALE Leslie GREINER
金额：
$ 18.19万
依托单位：
UNIV OF MASSACHUSETTS MED SCH WORCESTER
依托单位国家：
美国
项目类别：
财政年份：
2010
资助国家：
美国
起止时间：
2010-06-01 至 2015-05-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7994929
关键词：
Adoptive Transfer Affect Agonist Animal Housing Animal Model Animals Area Autopsy Beds Biological Models Biopsy Bone Marrow Cells Cells Charge Clinic Ensure Evaluation Facility Designs Food Graft Survival Hematopoietic Housing Human Resources Individual Infection Infection Control Infectious Agent Instruction Islets of Langerhans Transplantation Laboratories Maintenance Mature T-Lymphocyte Medicine Monitor Monoclonal Antibodies Mus Operative Surgical Procedures Outcome Patients Pharmaceutical Preparations Population Prevention Procedures Quality Control Reagent Records Recovery Research Personnel Rivers Serologic tests Serological Services Skin Skin Transplantation Skin graft Source Specimen Spleen Systems Analysis Techniques Training Transgenic Organisms Transplantation Virus Virus Diseases Water biocontainment facility data integrity in vivo lymph nodes novel peripheral blood programs research study self-renewal skin allograft success

项目摘要

The Mouse and Transplantation Core will have overall responsibilities for training all Projects in the transplantation of skin allografts and monitoring for graft survival. It will also have responsibility for maintenance, treatment, and surgical manipulation of virus treated experimental animals in BSL2/3 level biocontainment. All Program Project experiments will utilize VAF animals housed in a VAF facility or in a limited access biocontainment facility designed to maintain the highest degree of cleanliness. The emphasis on strict limited-access biocontainment will preclude cross-contamination and corruption of outcomes by unintended infectious agents. The objectives and responsibilities ofthe Animal Core Unit are as follows: By administering or training personnel in proper administration of reagents, including viruses, anti-CDI 54 mAb, other monoclonal antibodies, cells, TLR agonists, and other specific agents to be developed in the course ofthe Program to animals in VAF and in biocontainment. By preparing hematopoietic "synchimeras," an animal model system that is crucial for the success of Projects 1-3. ¿ Procurement of cells, biopsy and autopsy specimens for histological and flow cytometric analysis from animals in biocontainment. ¿ Animal Core personnel will perform or assist with performing skin grafts for all Projects. ¿ Serological testing of experimental animals to document that infection has occurred and that there is no cross-contamination. In brief, the Mouse and Transplantation Core will provide essential assistance and personnel training to each Project with the management and execution of in vivo studies conducted in defined VAF facilities and in strictly controlled infectious conditions in biocontainment.

小鼠和移植中心将全面负责培训中心的所有项目