PRENOLS AND OTHER LIPIDS

核心K(正丙醇和其他脂类)的目标是提供全面的分离方法 和鉴定小鼠巨噬细胞及其相关组织中不同的戊烯醇、心磷脂和新的次要脂类 在基础、刺激或药物治疗条件下的来源。该战略将涉及量化 已知的脂类，以及新型次要脂类的提纯和结构表征 质谱学和其他结构方法。核心K将与财团的其他核心合作 因为他们遇到了需要识别的新的脂类。核心K将与化学重点领域合作，以 确保新的结构方案通过及时的综合得到验证。在即将到来的拨款中 在此期间，核心K有以下具体目标，这些目标与集团的其他成员完全结合在一起： 具体目标1.使用脂类组学来促进对新陈代谢的机制理解， 尤其是涉及戊烯醇、心磷脂和新的次要脂类。 除了与联盟相关的目标之外，还描述了几种假设驱动的方法 新发现的脂类家族A/-酰基磷脂酰丝氨酸的生物合成和功能研究 在最后一次授权期内。 特定目标2：应用脂类组学研究病理状态下的巨噬细胞和组织 疾病模型，重点是戊烯醇、心磷脂和新的次要脂类。 除了与联盟相关的目标外，还描述了以假设为驱动的方法来阐明 脑神经黑素颗粒中发现的新戊烯醇--棕榈酸的生物合成和功能。 具体目标3：从脂类组学数据分析中开发脂类网络和图谱，重点是 戊烯醇、心磷脂和新的次要脂类。 除了与联盟相关的AIMS外，Core K还将为脂类提供注释路径 模型系统，如大肠杆菌和酵母菌，它们不是脂谱的焦点，但对 揭示脂质生物合成和遗传学的新机制。 全面了解脂类的结构和功能对于理解 重要疾病过程的机制，如动脉粥样硬化、炎症和糖尿病。这个 通过质谱学实现的血脂水平的定量测量也有助于评估 常用药物的治疗作用，如降胆固醇或胰岛素增敏剂。

The goal of Core K (Prenols and Other Lipids) is to provide comprehensive approaches to the isolation and identification of diverse prenols, cardiolipins and novel minor lipids in mouse macrophages and related sources under basal, stimulated or drug-treated conditions. The strategy will involve the quantification of known lipids, and the purification and structural characterization of novel minor lipids by state of the art ESI mass spectrometry and other structural methods. Core K will collaborate with other cores of the Consortium as they encounter new lipids in need of identification. Core K will work with the Chemistry Focus Area to ensure that novel structural proposals are validated by synthesis in a timely manner. In the coming grant period, Core K has the following specific aims, which are fully integrated with the rest of the Consortium: Specific Aim 1. Employ lipidomics to advance mechanistic understanding of metabolism, especially as it relates to prenols, cardiolipins and novel minor lipids. In addition to the Consortium-related aims, several hypothesis-driven approaches are described for elucidating the biosynthesis and function the A/-acyl-phosphatidylserines, a novel family of lipids discovered during the last grant period. Specific Aim 2: Employ lipidomics to investigate macrophages and tissues under pathological conditions as disease models, with emphasis on prenols, cardiolipins and novel minor lipids. In addition to the Consortium-related aims, hypothesis-driven approaches are described for elucidating the biosynthesis and function of the dolichoic acids, new prenols discovered in brain neuromelanin granules. Specific Aim 3: Develop lipid networks and maps from lipidomics data analysis, with a focus on prenols, cardiolipins and novel minor lipids. In addition to the Consortium-related aims, Core K will provide annotated pathways for lipid categories in model systems, like E. coli and yeast, which are not the focus of LIPID MAPS, but which are invaluable for uncovering new mechanisms of lipid biosynthesis and genetics. A comprehensive knowledge of the structure and function of lipids is crucial for understanding the mechanisms of important disease processes, such as atherosclerosis, inflammation and diabetes. The quantitative measurement of lipid levels enabled by mass spectrometry also facilitates the evaluation of the therapeutic actions of commonly used drugs, such as the cholesterol lowering or insulin sensitizing agents.