ADVANCED BIOSENSING FOR MOLECULAR AND CELLULAR BIOMARKERS

目前的医学测试主要是在条件良好的实验室中由训练有素的人员进行的， 获得稳定的电源和稳定的试剂供应。然而，在许多情况下，具有分析点或护理点能力是有价值的。例如，环境因素最好在现场进行评估 为了避免样本的运输，提供实时警告，当在第三世界国家工作时， 或在充满挑战和敌意的环境中工作时。该项目的长期目标是开发一个 这是一个完全集成的平台，用于在现场进行复杂的蛋白质测定。系统 包括一个可编程的微流体样品处理装置，称为细胞自动机， 新型霍尔效应电传感器。该分析系统将用于检测PAH-蛋白质加合物 致突变和致癌的挑战。然而，这个通用平台的开发 靶免疫检测还将广泛应用于生物医学研究、病原体和感染 疾病检测我们的总体目标将通过以下具体目标来实现：1.微流体 自动机将被开发，这是一个可编程的微流体设备，用于执行流体和样品 纳米级的制备操作。该设备将被开发和测试，以执行 进行PAH-蛋白加合物的基本光学检测ELISA分析所需的操作。2.同时， Boser小组将设计和制造一套先进的霍尔传感器芯片，这些芯片经过优化， 用自动机检测免疫过程。3.下一步将演示 用于检测PAH蛋白的自动样品制备与霍尔传感器的集成 加合物一旦证明检测试剂盒兼容，霍尔传感器将直接与 自动机，形成一个原型集成分析系统。最终目标是一个完全集成的电子 将开发接口和低成本一次性微流体盒和仪器， 预处理、孵育、与试剂混合以及在盒中自动检测分析物。 该项目利用微流体样品制备和PAH检测的最新技术 在本提案的项目1中开发。这些技术将导致用于早期诊断的设备和方法。 检测和更好的信息，这将导致更准确的诊断和有针对性的治疗。

Present medical tests are largely performed in conditioned laboratories by highly trained personel with access to stable power and a constant supply of reagents. In many situations, however, it is valuable to have point-of-analysis or point-of-care capabilities. For example, environmental factors may best be evaluated onsite to avoid transportation of samples, to provide a real-time warning, when working in third world countries, or when working in challenging and hostile environments. The long term aim of this project is to develop a paradigm for a fully integrated platform for performing complex protein assays in the field. The system consists of a programmable microfluidic sample processing device called a cellular automaton coupled to a novel Hall-effect electrical sensor. This analysis system will be developed to detect PAH-protein adducts that mutagenic and carcenogenic challenges. However, the development of this general platform for immunodetection of targets will also find wide application in biomedical research, pathogen and infectious disease detection. Our overall goal will be addressed by the following specific aims: 1. A microfluidic automaton will be developed that is a programmable microfluidic device for performing fluidic and sample preparation operations at the nanoliter scale. This device will be developed and tested to perform the operations necessary to do basic optically detected ELISA analyzes of PAH-protein adducts. 2. In parallel, the Boser group will design and fabricate a set of advanced Hall Sensor chips that are optimized for mating with the automaton and detecting the immunoassay process. 3. The next step will demonstrate the integration of the automaton sample preparation with the Hall sensor for the detection of PAH-protein adducts. Once the assays are demonstrated to be compatible, the Hall sensor will be directly coupled with the automaton to form a prototype integrated analysis system. In the final aim a fully integrated electronic interface and low-cost disposable microfluidic cartridges and instrument will be developed to perform sample preprocessing, incubation, mixing with reagents, and detection of the analyte automatically in the cartridge. This project leverages microfluidic sample preparation and state-of-the-art detection with the PAH assay developed in Project 1 of this proposal. These technologies will lead to devices and methods for early detection and better information that will lead to more accurate diagnosis and targeted treatments.