ADVANCED BIOSENSING FOR MOLECULAR AND CELLULAR BIOMARKERS

适用于分子和细胞生物标志物的先进生物传感

• 批准号: 7630608
• 负责人: Bernhard E Boser
• 金额: $ 37.92万
• 依托单位: UNIVERSITY OF CALIFORNIA BERKELEY
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7630608
• 关键词: Address; Antibodies; Arts; Binding; Biological Assay; Biological Markers; Biomedical Research; Biosensing Techniques; Blood; Calibration; Chemistry; Collaborations; Communicable Diseases; Compatible; Complex; Computers; Condition; Coupled; Coupling; Detection; Developing Countries; Development; Devices; Diagnosis; Early Diagnosis; Electronics; Environment; Environmental Exposure; Environmental Risk Factor; Enzyme-Linked Immunosorbent Assay; Evaluation; Goals; Immunoassay; Individual; Label; Laboratories; Lead; Magnetism; Measures; Medical; Methods; Microfluidic Microchips; Microfluidics; Modeling; Modification; Molecular; Numbers; Operative Surgical Procedures; Optics; Partner in relationship; Performance; Plastics; Preparation; Process; Proteins; Range; Reader; Reagent; Research Personnel; Route; Sampling; Scientist; Semiconductors; Serum; Signal Transduction; Solutions; Spottings; Standards of Weights and Measures; Structure; System; Systems Analysis; Technology; Testing; Time; Training; Transportation; Work; absorption; adduct; analog; cost; design; detector; digital; human disease; improved; instrument; laptop; metal oxide; micro-total analysis system; nanolitre scale; novel; pathogen; point of care; prototype; research study; scale up; sensor; size

Present medical tests are largely performed in conditioned laboratories by highly trained personel with access to stable power and a constant supply of reagents. In many situations, however, it is valuable to have point-of-analysis or point-of-care capabilities. For example, environmental factors may best be evaluated onsite to avoid transportation of samples, to provide a real-time warning, when working in third world countries, or when working in challenging and hostile environments. The long term aim of this project is to develop a paradigm for a fully integrated platform for performing complex protein assays in the field. The system consists of a programmable microfluidic sample processing device called a cellular automaton coupled to a novel Hall-effect electrical sensor. This analysis system will be developed to detect PAH-protein adducts that mutagenic and carcenogenic challenges. However, the development of this general platform for immunodetection of targets will also find wide application in biomedical research, pathogen and infectious disease detection. Our overall goal will be addressed by the following specific aims: 1. A microfluidic automaton will be developed that is a programmable microfluidic device for performing fluidic and sample preparation operations at the nanoliter scale. This device will be developed and tested to perform the operations necessary to do basic optically detected ELISA analyzes of PAH-protein adducts. 2. In parallel, the Boser group will design and fabricate a set of advanced Hall Sensor chips that are optimized for mating with the automaton and detecting the immunoassay process. 3. The next step will demonstrate the integration of the automaton sample preparation with the Hall sensor for the detection of PAH-protein adducts. Once the assays are demonstrated to be compatible, the Hall sensor will be directly coupled with the automaton to form a prototype integrated analysis system. In the final aim a fully integrated electronic interface and low-cost disposable microfluidic cartridges and instrument will be developed to perform sample preprocessing, incubation, mixing with reagents, and detection of the analyte automatically in the cartridge. This project leverages microfluidic sample preparation and state-of-the-art detection with the PAH assay developed in Project 1 of this proposal. These technologies will lead to devices and methods for early detection and better information that will lead to more accurate diagnosis and targeted treatments.

目前的医学测试主要是由训练有素的人员在有条件的实验室中进行的，这些人员可以获得稳定的电力和持续的试剂供应。然而，在许多情况下，具有分析点或护理点功能是很有价值的。例如，环境因素最好在现场进行评估。 为了避免样品的运输，为了提供实时警告，当在第三世界国家工作时， 或在充满挑战和恶劣的环境中工作时。这个项目的长期目标是开发一种 这是一个完全集成的平台，用于现场进行复杂的蛋白质分析。系统 由一个称为细胞自动机的可编程微流控样品处理设备组成，该设备耦合到 新型霍尔效应电感应器。该分析系统将用于检测多环芳烃-蛋白质加合物 诱变和致癌的挑战。然而，这个通用平台的开发 靶标的免疫检测也将在生物医学研究、病原体和传染性疾病中得到广泛应用 疾病检测。我们的总体目标将通过以下具体目标来实现：1.微流控 将开发Automaton，这是一种可编程的微流控设备，用于执行流体和样品 纳升级别的制备操作。该设备将被开发和测试以执行 对多环芳烃-蛋白质加合物进行基本光学检测的必要操作。2.同时， 博世集团将设计和制造一套先进的霍尔传感器芯片，这些芯片针对配对进行了优化 自动机和检测免疫分析过程。3.下一步将演示 自动机制样与霍尔传感器相结合检测多环芳烃蛋白质 加合物。一旦检测结果被证明是兼容的，霍尔传感器将直接与 该自动机形成了一个原型集成分析系统。最终的目标是一个完全集成的电子产品 将开发接口和低成本一次性微流控色谱柱和仪器来进行采样 对色谱柱中的分析物进行自动处理、孵化、与试剂混合和检测。 该项目利用微流控样品制备和最先进的多环芳烃检测技术 在本建议书的项目1中开发。这些技术将导致早期的设备和方法 检测和更好的信息将导致更准确的诊断和有针对性的治疗。