Generation of HIV resistant cells from protein transduced hematopoetic stem cell

描述(申请人提供)：使用逆转录病毒基因转导的方法，我们已经能够从原代小鼠血液干细胞中产生条件永生化的长期造血干细胞系。该项目的第一阶段包括应用这项技术来产生人类细胞系。此外，该项目还包括测试一种从小鼠身上产生干细胞系的非病毒方法。该项目的第二阶段将需要优化从对艾滋病毒感染具有抵抗力的人类细胞中大规模生产蛋白质转导的长期造血干细胞，以便在更大的动物和人类临床试验中进行最终测试。 公共卫生相关性：该项目寻求优化一种新的方法，以产生大量的人类血液干细胞，用于临床预防和治疗艾滋病毒感染。一旦这种方法被验证用于大规模生产，受益于公共卫生的长期目标将是使用该系统在受感染的个人或在有感染艾滋病毒风险的人群中产生抗艾滋病毒的细胞。

DESCRIPTION (provided by applicant): Using a method of retroviral gene transduction we have been able to generate conditionally immortalized long-term hematopoietic stem cell lines from primary mouse blood stem cells. Phase I of this project included applying this technology to generate human cell lines. In addition, the project included testing a non-viral method to generate the stem cell lines from mice. Phase II of the project will entail optimizing large-scale production of protein transduced long-term hematopoietic stem cells from human cells that are resistant to HIV infection for eventual testing in larger animals and human clinical trials. PUBLIC HEALTH RELEVANCE: This project seeks to optimize a novel method to generate large amounts of human blood stem cells for clinical use in the prevention and treatment of HIV infection. Once this method has been validated for large-scale production, the long-term goal for benefiting public health will be to use this system to generate HIV resistant cells in infected individuals or in populations that are at-risk for acquiring the HIV virus.