COPY NUMBER VARIANTS AND EARLY-ONSET BREAST CANCER

拷贝数变异和早发性乳腺癌

• 批准号: 8107328
• 负责人: Paul Joseph Goodfellow
• 金额: $ 62.14万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-09-06 至 2012-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8107328
• 关键词: Accounting; Affect; Age; Alleles; Attention; BRCA1 gene; BRCA2 gene; Breast Cancer Treatment; Cancer Patient; Candidate Disease Gene; Custom; Data; Detection; Development; Diagnosis; Disease; Environmental Risk Factor; Family; Family Study; Family history of; Family member; Frequencies; Future; Genes; Genetic Predisposition to Disease; Genetic Risk; Genetic Variation; Genome; Goals; Hereditary Disease; Hormones; Inherited; Learning; Malignant Neoplasms; Maps; Methods; Minor; Modeling; Mutation; Mutation Analysis; Outcome; Parents; Pathway interactions; Play; Population; Predisposition; Prevention strategy; Research; Resolution; Resources; Risk; Role; Sister; TP53 gene; Testing; Variant; Woman; Work; advanced disease; breast cancer diagnosis; breast tumorigenesis; cancer risk; cohort; cost effective; density; early onset; experience; follow-up; genetic pedigree; genetic risk factor; human disease; interest; loss of function; malignant breast neoplasm; member; novel; proband; segregation; tumor

DESCRIPTION (provided by applicant): Breast cancer takes its greatest toll on young women. Young women frequently have biologically aggressive tumors. They often present with advanced disease and their tumors are frequently hormone non-responsive, thereby limiting treatment options. Young women suffer lower than average disease-free and overall survival. The work proposed is focused on discovery of the as yet unknown genetic risk factors that underlie development of early-onset breast cancer. These findings will pave the way for future studies to elucidate how genetic risk and environmental factors interact and account for the aggressive tumors and poor outcome young breast cancer patients experience. We hypothesize copy number variants (CNVs) play an important role in risk for development of early-onset breast cancer. Three inter-related aims are proposed to identify the inherited CNVs and genes they impact that are important in early-onset breast cancer. Aim 1. Assess copy number variants (CNVs) in 120 BRCA1 and BRCA2 negative breast cancer patients diagnosed <40 along with their biologic parents. We will map CNVs at high density in the genomes of 120 early-onset breast cancer patients, comparing their CNV make-up with their parents'. Aim 2. Validate CNVs and evaluate an additional cohort of early-onset breast cancer patients for variants identified in Aim 1. CNVs identified in Aim 1 will be validated using a high resolution custom array to identify candidate breast cancer susceptibility loci. A total of 240 cases will be investigated. Variant-specific PCR amplicons will be developed for a select set of CNVs. Aim 3. Characterize CNVs and candidate genes to determine their role in breast cancer risk. Mutation analysis will be our primary method for determining if a CNV-associated candidate gene is involved in breast cancer risk. Mutations seen in breast cancer cases but not in controls would be taken as evidence for a gene's role in breast cancer susceptibility. We will investigate families for co-segregation of CNVs with cancer and use family member DNAs to verify and refine interpretation of allelism for CNVs of interest. PUBLIC HEALTH RELEVANCE: The potential impact for the work proposed is the development of new strategies for the prevention, detection and treatment of breast cancer that will come from learning what additional genes and pathways are important in breast tumorigenesis and those that are particularly relevant to early-onset breast cancer.

描述（由申请人提供）：乳腺癌对年轻女性的影响最大。年轻女性经常患有生物侵袭性肿瘤。他们经常出现晚期疾病，他们的肿瘤经常是激素无反应的，从而限制了治疗选择。年轻妇女的无病生存率和总生存率低于平均水平。这项工作的重点是发现导致早发性乳腺癌发生的未知遗传风险因素。这些发现将为未来的研究铺平道路，以阐明遗传风险和环境因素如何相互作用，并解释年轻乳腺癌患者经历的侵袭性肿瘤和不良结局。我们假设拷贝数变异（CNVs）在早发性乳腺癌的发生风险中起重要作用。提出了三个相互关联的目标，以确定遗传的CNV和基因，他们的影响，是重要的早发性乳腺癌。目标1.评估120例BRCA1和BRCA2阴性乳腺癌患者沿着生物学父母的拷贝数变异（CNV），这些患者被诊断为<40岁。我们将在120名早发性乳腺癌患者的基因组中绘制高密度的CNV图谱，并将他们的CNV组成与父母的CNV组成进行比较。目标2.检测CNV，并评估早发性乳腺癌患者的另一个队列，以确定目标1中的变异。将使用高分辨率定制阵列验证目标1中鉴定的CNV，以鉴定候选乳腺癌易感性基因座。将对240起案件进行调查。将为选定的一组CNV开发变体特异性PCR扩增子。目标3。表征CNV和候选基因，以确定它们在乳腺癌风险中的作用。突变分析将是我们确定CNV相关候选基因是否参与乳腺癌风险的主要方法。在乳腺癌病例中观察到的突变，而在对照组中没有，将被视为基因在乳腺癌易感性中作用的证据。我们将调查CNVs与癌症共分离的家族，并使用家族成员DNA来验证和完善对感兴趣的CNVs等位性的解释。 公共卫生相关性：这项工作的潜在影响是制定预防、检测和治疗乳腺癌的新策略，这将来自于了解哪些其他基因和途径在乳腺肿瘤发生中是重要的，以及那些与早发性乳腺癌特别相关的基因和途径。