Thermodynamic and kinetic studies of protein structure and enzymic mechanisms

蛋白质结构和酶机制的热力学和动力学研究

• 批准号: 8148698
• 负责人: Allen P Minton
• 金额: $ 30.67万
• 依托单位: NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8148698
• 关键词: Kinetics; Thermodynamics; protein structure

(i) Methods are being developed for the detection of very low concentrations of flourescently labeled proteins in the presence of very high concentrations of unlabeled proteins and polymers. These techniques will be used to investigate non-specific protein/protein and protein/polymer interactions in solution. (ii) Antibody presentation is a key step in the initiation of the adaptive immune response, depending on the expression of costimulatory molecules. Immune-enhancing oligodeoxynucleotides (ODNs)increase the expression of these molecules. CD measurements were used to show that several of these regulatory ODNs contain guanine(G)-tetrad structures. Heat treatment of these ODNs which diminishes their regulatory properties also destroyed the G-tetrads. (iii) Optical studies on the structures of amyloid peptides and proteins are continuing.We are particularly interested in origin of the very intense CD spectrum which has been associated with the cross-beta structure of amyloid fibrils.

(i)正在开发用于在存在非常高浓度的未标记蛋白质和聚合物的情况下检测非常低浓度的荧光标记蛋白质的方法。这些技术将用于研究溶液中的非特异性蛋白质/蛋白质和蛋白质/聚合物相互作用。 (ii)抗体呈递是启动适应性免疫应答的关键步骤，取决于共刺激分子的表达。 免疫增强性寡脱氧核苷酸（ODNs）增加这些分子的表达。CD测量用于显示这些调节ODN中的几个含有鸟嘌呤（G）-四联体结构。热处理这些ODNs，减少其调节性能也破坏了G-四分体。 (iii)对淀粉样肽和蛋白质结构的光学研究仍在继续，我们特别感兴趣的是与淀粉样纤维的交叉β结构相关的非常强的CD光谱的起源。