Thermodynamic and kinetic studies of protein structure and enzymic mechanisms

共振光散射是一种深奥的技术，它利用了一些发色团的高分子聚集体的吸收带内折射率的复杂行为。最近发表了许多生物化学分析，声称使用这种方法。我们已经证明，在这些实验中检测到的大多数散射峰是假的，这是由仪器的光电倍增管的特性引起的，而不是由所研究的分子种类引起的。本文描述了一种检测这种伪影的简单方法。（麦克菲P. 2006）

(i) Resonance light scattering is an esoteric technique, which takes advantage of the complex behavior of the refractive index within the absorbance bands of high molecular aggregates of some chromophores. Recently numerous biochemical assays have been published, which claim to use this methodology. We have shown that most of the scattering peaks detected in these experiments are spurious, arising from the properties of the intrument's photomultiplier, not from the molecular species under study. A simple method for the detection of this artifact has been descibed. (McPhie P. 2006) (ii) Antibody presentation is a key step in the initiation of the adaptive immune response, depending on the expression of costimulatory molecules. Immune-enhancing oligodeoxynucleotides (ODNs)increase the expression of these molecules. CD measurements were used to show that several of these regulatory ODNs contain guanine(G)-tetrad structures. Heat treatment of these ODNs which diminishes their regulatory properties also destroyed the G-tetrads. ((Wang J. et al 2007) (iii) Optical studies on the structures of amyloid peptides and proteins are continuing.