Consequences of developmental defects in somatosensory map formation

体感图形成发育缺陷的后果

• 批准号: 8792639
• 负责人: Reha S Erzurumlu
• 金额: $ 33.76万
• 依托单位: UNIVERSITY OF MARYLAND BALTIMORE
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-02-01 至 2019-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8792639
• 关键词: Affect; Animals; Auditory system; Axon; Behavior; Behavioral; Bilateral; Biology; Body Image; Brain; Calcium; Cell Nucleus; Cells; Cerebral cortex; Cerebral hemisphere; Communication; Congenital Abnormality; Contralateral; Coupled; DNA Sequence Alteration; Defect; Development; Dyes; Electrophysiology (science); Electroporation; Esthesia; FOS gene; Face; Gene Deletion; Gene Mutation; Head; Health; Human; Human Genetics; Image; Indium; Injury; Ipsilateral; Label; Lead; Light; Maps; Microscopy; Molecular; Motor; Motor Pathways; Movement; Mus; Mutation; Neocortex; Nerve; Neurons; Ocular dominance columns; Pathway interactions; Pattern; Perception; Phenotype; Pontine structure; Preparation; Property; Sensory; Sensory Process; Sensory Receptors; Side; Site; Somatosensory Cortex; Synapses; System; Techniques; Testing; Thalamic structure; Transgenic Mice; Vibrissae; Vision; Visual Cortex; Visual system structure; Weight; Work; afferent nerve; awake; barrel cortex; behavioral outcome; brain pathway; cortex mapping; density; developmental genetics; in utero; information processing; mouse model; postsynaptic; relating to nervous system; research study; response; sensorimotor system; sensory cortex; sensory input; skills; somatosensory; two-photon; voltage

DESCRIPTION (provided by applicant): Topographic representation of the sensory space in the brain is essential for sensory information processing and perception. The somatosensory and motor cortical maps in each hemisphere represent the contralateral body and the face. This is due to midline crossing of the ascending (sensory) and descending (motor) pathways at the level of the medulla or the pons. Genetic and developmental defects in midline crossing or injury at the crossing site severely affect sensory-motor information processing and actions in both animals and humans. In this proposal we use a region-specific gene deletion mouse model to study the consequences of partial crossing of the ascending somatosensory face pathway. Midline crossing defects in this mouse leads to bilateral face representation in the thalamus and subsequently in the somatosensory cortex. We will use this mouse model to investigate (a) morphological and electrophysiological properties of the pre and postsynaptic elements in the "bifacial" cortical map; (b) altered thalamocortical and corticocortical connectivity patterns in response to bilateral face representation; (c) behavioral consequences of this genetic mutation. A combination of molecular, morphological, electrophysiological, voltage-sensitive dye imaging and behavioral techniques will be used to elucidate mechanisms underlying the functional organization and behavioral manifestations of developmental injury-related or genetic defects in ascending somatosensory pathways.

描述（由申请人提供）：大脑中感觉空间的地形表征对于感觉信息的处理和感知至关重要。每个半球的体感和运动皮层图代表对侧身体和面部。这是由于上升（感觉）和下降（运动）通路在髓质或脑桥水平的中线交叉。中线交叉的遗传和发育缺陷或交叉部位的损伤严重影响动物和人类的感觉-运动信息加工和动作。在这个建议中，我们使用区域特异性基因缺失小鼠模型来研究上升体感面部通路部分交叉的后果。这只小鼠的中线交叉缺陷导致丘脑和随后的体感皮层的双侧面部表征。我们将使用该小鼠模型来研究(a)“双面”皮层图中突触前和突触后元素的形态学和电生理特性；(b)双侧面部表征改变了丘脑皮质和皮质连接模式；(c)这种基因突变的行为后果。结合分子、形态学、电生理学、电压敏感染料成像和行为技术，将阐明在上升体感觉通路中发育损伤相关或遗传缺陷的功能组织和行为表现的机制。