Consequences of developmental defects in somatosensory map formation

体感图形成发育缺陷的后果

• 批准号: 8722646
• 负责人: Reha S Erzurumlu
• 金额: $ 35.1万
• 依托单位: UNIVERSITY OF MARYLAND BALTIMORE
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-02-01 至 2019-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8722646
• 关键词: Affect; Animals; Auditory system; Axon; Behavior; Behavioral; Behavioral Mechanisms; Bilateral; Biology; Body Image; Brain; Calcium; Cell Nucleus; Cells; Cerebral cortex; Cerebral hemisphere; Communication; Congenital Abnormality; Contralateral; Coupled; Defect; Development; Dyes; Electrophysiology (science); Electroporation; Esthesia; FOS gene; Face; Gene Deletion; Gene Mutation; Head; Human; Human Genetics; Image; Indium; Injury; Ipsilateral; Label; Lead; Light; Maps; Microscopy; Molecular; Motor; Motor Pathways; Movement; Mus; Mutation; Neocortex; Nerve; Neurons; Ocular dominance columns; Outcome; Pathway interactions; Pattern; Perception; Phenotype; Pontine structure; Preparation; Property; Sensory; Sensory Process; Sensory Receptors; Side; Site; Somatosensory Cortex; Synapses; System; Techniques; Testing; Thalamic structure; Transgenic Mice; Vibrissae; Vision; Visual Cortex; Visual system structure; Weight; Work; afferent nerve; awake; barrel cortex; brain pathway; density; developmental genetics; in utero; information processing; mouse model; postsynaptic; public health relevance; relating to nervous system; research study; response; sensorimotor system; sensory cortex; skills; somatosensory; two-photon; voltage

Topographic representation of the sensory space in the brain is essential for sensory information processing and perception. The somatosensory and motor cortical maps in each hemisphere represent the contralateral body and the face. This is due to midline crossing of the ascending (sensory) and descending (motor) pathways at the level of the medulla or the pons. Genetic and developmental defects in midline crossing or injury at the crossing site severely affect sensory-motor information processing and actions in both animals and humans. In this proposal we use a region-specific gene deletion mouse model to study the consequences of partial crossing of the ascending somatosensory face pathway. Midline crossing defects in this mouse leads to bilateral face representation in the thalamus and subsequently in the somatosensory cortex. We will use this mouse model to investigate (a) morphological and electrophysiological properties of the pre and postsynaptic elements in the "bifacial" cortical map; (b) altered thalamocortical and corticocortical connectivity patterns in response to bilateral face representation; (c) behavioral consequences of this genetic mutation. A combination of molecular, morphological, electrophysiological, voltage-sensitive dye imaging and behavioral techniques will be used to elucidate mechanisms underlying the functional organization and behavioral manifestations of developmental injury-related or genetic defects in ascending somatosensory pathways.

大脑中感觉空间的地形表征对于感觉信息至关重要 处理和感知。每个半球的体感和运动皮质图代表 对侧身体和面部。这是由于上升（感觉）和下降的中线交叉所致。 延髓或脑桥水平的（运动）通路。中线遗传和发育缺陷 穿越或穿越地点的伤害严重影响感觉运动信息处理和行动 动物和人类。在本提案中，我们使用区域特异性基因缺失小鼠模型来研究 部分穿越上升体感面部通路的后果。中线交叉缺陷 该小鼠在丘脑中产生双侧面部表征，随后在体感中产生双侧面部表征 皮质。我们将使用该小鼠模型来研究 (a) 的形态学和电生理学特性 “双面”皮质图中的突触前和突触后元素； (b) 丘脑皮质和皮质皮质改变 响应双边面部表征的连接模式； (c) 这种遗传的行为后果 突变。 分子、形态学、电生理学、电压敏感染料成像和 行为技术将用于阐明功能组织和基础机制 上行体感发育损伤相关或遗传缺陷的行为表现 途径。