TISSUE CULTURE/ MAb CORE

组织培养/单克隆抗体核心

基本信息

批准号：
8616657
负责人：
STEVEN M ANDERSON
金额：
$ 14.46万
依托单位：
UNIVERSITY OF COLORADO DENVER
依托单位国家：
美国
项目类别：
财政年份：
2014
资助国家：
美国
起止时间：
2014-02-05 至 2017-01-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8616657
关键词：
Antigens Back Baculoviruses Basic Science Biochemical Biological Assay Biological Markers Bioreactors Budgets Cancer Center Support Grant Cell Culture Techniques Cell Line Cells Charge Communities Consultations Enzyme-Linked Immunosorbent Assay Funding Generations Human Hybridomas Incubators Individual Insecta Institution Instruction Liquid substance Malignant Neoplasms Molecular Monoclonal Antibodies Nitrogen Preparation Production Property Proteins Reader Reagent Recombinant Proteins Recombinants Research Research Personnel Resources Services Source System Technology Tissue Culture Shared Resource Training Translational Research Tumor Cell Line Validation anticancer research cost cost effective drug related cancer hybridoma production interest meetings member monoclonal antibody production novel programs protein expression tissue culture vector

项目摘要

PROJECT SUMMARY (See instructions): Objective: The Protein Production, Monoclonal Antibody, Tissue Culture Shared Resource (PPSR) supports basic and translational research at UCCC with validated cell lines, preparation of hybridomas, production of monoclonal antibodies, isolation and titering of baculovirus expressing recombinant proteins, and large scale preparation of recombinant proteins and monoclonal antibodies. Services & Technologies: PPSR provides assays pertinent to cancer research, including generation of hybridomas with desired properties to novel antigens, production of monoclonal antibodies, production of recombinant baculovirus that express proteins of interest, large scale cultivation of cells of varying types, and validated tumor cell lines known to be free of contaminants. In FY2010, 74 human tumor cell lines have been validated for use by UCC investigators. The facility has biosafety cabinets, tissue culture incubators, an ELISA plate reader, bioreactors including three GE Wave bioreactors for large scale cultivation of cells, refrigerators, freezers, and liquid nitrogen storage systems for maintaining stocks of cell lines. Consultation & Training: PPSR provides consultation and technical support to help members prepare and isolate hybridomas that produce monoclonal antibodies with the desired properties directed against their proteins of interest. The facility also provides consultation and technical support for preparation, isolation, and production of recombinant baculovirus vectors encoding proteins of interest to support biochemical, molecular, and structural studies by UCCC members, including large volume (0.5 to 10 I) cultures of insect cells producing the protein of interest. Validated tumor cell lines are maintained by the PPSR allowing UCCC investigators to utilize cell lines known to reflect the original tumor cell line, and to be free of contaminating species, in their research. Utilization: PPSR has served 48 UCCC members from all 6 Programs and 3 consortium institutions. The majority of the reagents and cell lines provided by the PPSR support multiple basic and translational projects. Management and Finances: This resource is UCCC-managed. 61% of the operating budget comes from charge backs to UCCC users who represent 76% of the total users. PPSR requests ~$300K CCSG support for 48% of its operating budget. This increase represents the continuation of the cell line validation service currently supported by ARRA funding. Increased funding will continue our ability to meet the needs of the cancer research community to generate and utilize recombinant proteins, monoclonal antibodies, and provide the community with validated tumor cell lines free of contaminants for use in basic and translational research programs.

项目摘要（请参阅说明）：目的：蛋白质产生，单克隆抗体，组织培养共享资源（PPSR）支持UCCC的基本和转化研究，并具有经过验证的细胞系，杂交瘤的制备，单克隆抗体的产生，隔离和baculovirus titering baculovirus of Baculovirus表达重组蛋白，以及大尺度抗形素的蛋白质和Monococine蛋白和Monocion蛋白质和Monocion蛋白质和Monocion蛋白质的蛋白质。服务和技术：PPSR提供与癌症研究相关的测定，包括与新型抗原具有所需特性的杂交瘤，产生单克隆抗体的产生，产生重组杆状病毒，表达感兴趣的蛋白质，大规模的细胞培养各种类型的细胞，以及已知的具有验证肿瘤细胞系的细胞，是自由的。在2010财年，已经对UCC研究人员进行了验证74种人类肿瘤细胞系。该设施具有生物安全橱柜，组织培养孵化器，ELISA板板读取器，包括三个的生物反应器 GE波生物反应器，用于大规模培养细胞，冰箱，冷冻机和液氮储存系统，用于维持细胞系的库存。咨询与培训：PPSR提供咨询和技术支持，以帮助成员准备和隔离杂交瘤，这些杂种可产生单克隆抗体，并具有针对其感兴趣蛋白质的所需特性。该设施还为编码的重组杆状病毒载体的准备，隔离和生产提供咨询和技术支持 UCCC成员支持生化，分子和结构研究的感兴趣蛋白质，包括大容量（0.5至10 i）产生感兴趣蛋白质的昆虫细胞培养物。 PPSR维持经过验证的肿瘤细胞系，使UCCC研究人员可以利用已知的细胞系来反映原始的肿瘤细胞系，并在其研究中不含污染物。利用：PPSR已为所有6个计划和3个财团机构的48名UCCC成员提供服务。大多数试剂和细胞 PPSR提供的线支持多个基本和翻译项目。管理和财务：此资源由UCCC管理。 61％的运营预算来自支付给UCCC用户的收费，这些用户占总用户的76％。 PPSR要求约30万美元的CCSG支持其运营预算的48％。此增加代表了ARRA资金当前支持的单元线验证服务的延续。增加的资金将继续我们满足癌症研究界的需求，以生成和利用重组蛋白，单克隆抗体，并为社区提供经过验证的肿瘤细胞系，无污染物用于基础和转化研究计划。