TISSUE CULTURE/ MAb CORE

项目总结（见说明）： 目的：蛋白质生产，单克隆抗体，组织培养共享资源（PPSR）支持UCCC的基础和转化研究，包括经验证的细胞系，杂交瘤制备，单克隆抗体生产，表达重组蛋白的杆状病毒的分离和滴定，以及重组蛋白和单克隆抗体的大规模制备。服务与技术：PPSR提供与癌症研究相关的测定，包括产生具有针对新抗原的所需特性的杂交瘤、产生单克隆抗体、产生表达目的蛋白的重组杆状病毒、大规模培养不同类型的细胞以及已知不含污染物的经验证的肿瘤细胞系。在2010财政年度，74种人类肿瘤细胞系已被UCC研究者验证使用。该设施有生物安全柜，组织培养孵育器，ELISA平板阅读器，生物反应器，包括三个 用于大规模细胞培养的GE Wave生物反应器、用于维持细胞系储备的冰箱、冷冻机和液氮储存系统。咨询与培训：PPSR提供咨询和技术支持，以帮助成员制备和分离杂交瘤，产生针对其感兴趣的蛋白质的所需特性的单克隆抗体。该机构还为重组杆状病毒载体的制备、分离和生产提供咨询和技术支持， 目的蛋白质，以支持UCCC成员的生物化学，分子和结构研究，包括产生目的蛋白质的昆虫细胞的大体积（0.5至10升）培养物。经验证的肿瘤细胞系由PPSR保存，允许UCCC研究人员在其研究中使用已知反映原始肿瘤细胞系的细胞系，并且不含污染物种。利用率：PPSR已为来自所有6个项目和3个联盟机构的48个UCCC成员提供服务。大多数试剂和细胞 PPSR提供的行支持多个基本和翻译项目。管理和财务： 此资源由UCCC管理。61%的运营预算来自对UCCC用户的收费，这些用户占总用户的76%。PPSR要求约30万美元的CCSG支持其运营预算的48%。这一增长代表了目前由ARRA资金支持的细胞系验证服务的继续。增加的资金将继续我们的能力，以满足癌症研究界的需求，以产生和利用重组蛋白，单克隆抗体，并提供社区与验证的肿瘤细胞系无污染物用于基础和转化研究计划。

PROJECT SUMMARY (See instructions): Objective: The Protein Production, Monoclonal Antibody, Tissue Culture Shared Resource (PPSR) supports basic and translational research at UCCC with validated cell lines, preparation of hybridomas, production of monoclonal antibodies, isolation and titering of baculovirus expressing recombinant proteins, and large scale preparation of recombinant proteins and monoclonal antibodies. Services & Technologies: PPSR provides assays pertinent to cancer research, including generation of hybridomas with desired properties to novel antigens, production of monoclonal antibodies, production of recombinant baculovirus that express proteins of interest, large scale cultivation of cells of varying types, and validated tumor cell lines known to be free of contaminants. In FY2010, 74 human tumor cell lines have been validated for use by UCC investigators. The facility has biosafety cabinets, tissue culture incubators, an ELISA plate reader, bioreactors including three GE Wave bioreactors for large scale cultivation of cells, refrigerators, freezers, and liquid nitrogen storage systems for maintaining stocks of cell lines. Consultation & Training: PPSR provides consultation and technical support to help members prepare and isolate hybridomas that produce monoclonal antibodies with the desired properties directed against their proteins of interest. The facility also provides consultation and technical support for preparation, isolation, and production of recombinant baculovirus vectors encoding proteins of interest to support biochemical, molecular, and structural studies by UCCC members, including large volume (0.5 to 10 I) cultures of insect cells producing the protein of interest. Validated tumor cell lines are maintained by the PPSR allowing UCCC investigators to utilize cell lines known to reflect the original tumor cell line, and to be free of contaminating species, in their research. Utilization: PPSR has served 48 UCCC members from all 6 Programs and 3 consortium institutions. The majority of the reagents and cell lines provided by the PPSR support multiple basic and translational projects. Management and Finances: This resource is UCCC-managed. 61% of the operating budget comes from charge backs to UCCC users who represent 76% of the total users. PPSR requests ~$300K CCSG support for 48% of its operating budget. This increase represents the continuation of the cell line validation service currently supported by ARRA funding. Increased funding will continue our ability to meet the needs of the cancer research community to generate and utilize recombinant proteins, monoclonal antibodies, and provide the community with validated tumor cell lines free of contaminants for use in basic and translational research programs.