Functional Development of the Mammary Gland

乳腺的功能发育

基本信息

  • 批准号:
    8062791
  • 负责人:
  • 金额:
    $ 23.64万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2010
  • 资助国家:
    美国
  • 起止时间:
    2010-09-01 至 2011-08-31
  • 项目状态:
    已结题

项目摘要

It is the recommendation of the American Academy of Pediatrics that all children be breast-fed for at least the first six months of life [2], Despite this recommendation, the functional development of the mammary gland and the result of that development, milk secretion, have received relatively little attention over the past two decades. In this program project we seek to remedy this deficit by bringing together six well-qualified investigators with varying expertise to examine four critical aspects of the regulation and mechanism of milk secretion. In Project I we will examine a new hypothesis that links balanced glycolysis in the mammary gland both to lipid synthesis and cell survival. We hypothesize that changes in the activity/conformation of hexokinase regulated by the serine/threonine kinase Akt/PKB provide the crucial link between these two functions. We will test the hypothesis in genetically altered mice. In Project II, we will examine the molecular and cell biological basis of the formation of cytoplasmic lipid droplets (CLD) in the mammary alveolar cell, testing the hypothesis that these entities originate within specialized domains in the endoplasmic reticulum containing all the necessary synthetic machinery machine for their synthesis. In project III, we will scrutinize the mechanisms by which progesterone inhibits the initiation of milk secretion in late pregnancy examining both direct and indirect mechanisms and their localization in the mammary gland to progesterone receptor containing cells and other cells. Strains of genetically altered mice in which the LacZ has been targeted to the progesterone receptor coding region and green fluorescent protein to the b-casein coding region will be used to accomplish this aim. In Project IV we will initiate studies of the role of PKC* on cell proliferation and apoptosis in pregnancy, lactation and involution. T his serine/threonine kinase is an upstream regulatory factor in epithelial cell apoptosis and we will use it to elucidate the pathways through which mammary epithelial cell survival can be compromised by milk stasis or hormone withdrawal. These studies will form a foundation on which to base an understanding of why lactation does not always proceed optimally and in the long run should help us increase the human milk supply for infants, particularly preterm infants, for whom human breast milk is a priority.
美国儿科学会建议所有儿童至少在出生后的前六个月进行母乳喂养[2],尽管有这一建议,但乳腺的功能发育及其结果,即乳汁分泌,在过去二十年中受到的关注相对较少。在这个项目中,我们试图通过汇集六名具有不同专业知识的合格研究人员来研究乳汁分泌的调节和机制的四个关键方面来弥补这一缺陷。在项目I中,我们将研究一个新的假设,即乳腺糖酵解平衡与脂质合成和细胞存活之间的联系。我们推测丝氨酸/苏氨酸激酶Akt/PKB调节的己糖激酶活性/构象的变化提供了这两种功能之间的关键联系。我们将在转基因小鼠中检验这一假设。在项目II中,我们将研究乳腺肺泡细胞中细胞质脂滴(CLD)形成的分子和细胞生物学基础,测试这些实体起源于内质网中包含所有必要的合成机器机器的专门领域的假设。在项目III中,我们将详细研究孕激素抑制妊娠晚期乳汁分泌的机制,检查直接和间接机制及其在乳腺中的孕激素受体细胞和其他细胞的定位。将使用基因改变的小鼠品系来实现这一目的,其中LacZ已经靶向孕酮受体编码区,绿色荧光蛋白靶向b-酪蛋白编码区。在项目IV中,我们将开始研究PKC* 在妊娠、哺乳和退化中对细胞增殖和凋亡的作用。他的丝氨酸/苏氨酸激酶是上皮细胞凋亡的上游调控因子,我们将用它来阐明乳腺上皮细胞的生存可以通过乳汁淤积或激素戒断损害的途径。这些研究将为理解为什么哺乳并不总是以最佳方式进行奠定基础,从长远来看,这些研究将有助于我们增加婴儿的母乳供应,特别是早产儿,母乳是他们的优先事项。

项目成果

期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ monograph.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ sciAawards.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ conferencePapers.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ patent.updateTime }}

STEVEN M ANDERSON其他文献

STEVEN M ANDERSON的其他文献

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

{{ truncateString('STEVEN M ANDERSON', 18)}}的其他基金

Lifestyle associated reactive metabolites and their negative impact on breast cancer risk
生活方式相关的反应性代谢物及其对乳腺癌风险的负面影响
  • 批准号:
    10206074
  • 财政年份:
    2020
  • 资助金额:
    $ 23.64万
  • 项目类别:
TISSUE CULTURE/ MAb CORE
组织培养/单克隆抗体核心
  • 批准号:
    8616657
  • 财政年份:
    2014
  • 资助金额:
    $ 23.64万
  • 项目类别:
Is GLUT1 required for tumor growth and the Warburg Effect?
肿瘤生长和瓦尔堡效应需要 GLUT1 吗?
  • 批准号:
    8505396
  • 财政年份:
    2011
  • 资助金额:
    $ 23.64万
  • 项目类别:
Endocrine Network for Undergraduate Research and Carrier Development Opportunitie
本科生研究和载体发展机会内分泌网络
  • 批准号:
    8626413
  • 财政年份:
    2011
  • 资助金额:
    $ 23.64万
  • 项目类别:
Is GLUT1 required for tumor growth and the Warburg Effect?
肿瘤生长和瓦尔堡效应需要 GLUT1 吗?
  • 批准号:
    8188853
  • 财政年份:
    2011
  • 资助金额:
    $ 23.64万
  • 项目类别:
Endocrine Network for Undergraduate Research and Carrier Development Opportunitie
本科生研究和载体发展机会内分泌网络
  • 批准号:
    8821630
  • 财政年份:
    2011
  • 资助金额:
    $ 23.64万
  • 项目类别:
Endocrine Network for Undergraduate Research and Carrier Development Opportunitie
本科生研究和载体发展机会内分泌网络
  • 批准号:
    8233329
  • 财政年份:
    2011
  • 资助金额:
    $ 23.64万
  • 项目类别:
Is GLUT1 required for tumor growth and the Warburg Effect?
肿瘤生长和瓦尔堡效应需要 GLUT1 吗?
  • 批准号:
    8333385
  • 财政年份:
    2011
  • 资助金额:
    $ 23.64万
  • 项目类别:
Endocrine Network for Undergraduate Research and Carrier Development Opportunitie
本科生研究和载体发展机会内分泌网络
  • 批准号:
    8460849
  • 财政年份:
    2011
  • 资助金额:
    $ 23.64万
  • 项目类别:
Endocrine Network for Undergraduate Research and Carrier Development Opportunitie
本科生研究和载体发展机会内分泌网络
  • 批准号:
    8013376
  • 财政年份:
    2011
  • 资助金额:
    $ 23.64万
  • 项目类别:

相似海外基金

Clinical application of boron-conjugated adenovirus vector for neutron capture therapy
硼缀合腺病毒载体中子捕获治疗的临床应用
  • 批准号:
    19K09482
  • 财政年份:
    2019
  • 资助金额:
    $ 23.64万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Vascular-targeted gene therapy to block proliferation of smooth muscle cells using a novel adenovirus vector
使用新型腺病毒载体进行血管靶向基因治疗以阻止平滑肌细胞增殖
  • 批准号:
    2273599
  • 财政年份:
    2019
  • 资助金额:
    $ 23.64万
  • 项目类别:
    Studentship
Gene therapy for diabetes mellitus based on the suppression of lipotoxicity using an improved adenovirus vector
使用改进的腺病毒载体抑制脂毒性的糖尿病基因治疗
  • 批准号:
    18K14964
  • 财政年份:
    2018
  • 资助金额:
    $ 23.64万
  • 项目类别:
    Grant-in-Aid for Early-Career Scientists
Mechanisms of induction of mucosal immunity by adenovirus vector vaccine
腺病毒载体疫苗诱导粘膜免疫的机制
  • 批准号:
    16K18873
  • 财政年份:
    2016
  • 资助金额:
    $ 23.64万
  • 项目类别:
    Grant-in-Aid for Young Scientists (B)
Hemophilia B Gene Therapy via CRISPR/Cas9-Targeted Integration of the Factor IX Gene using Adenovirus Vector Delivery
使用腺病毒载体递送通过 CRISPR/Cas9 靶向整合因子 IX 基因进行 B 型血友病基因治疗
  • 批准号:
    9193681
  • 财政年份:
    2016
  • 资助金额:
    $ 23.64万
  • 项目类别:
Gene therapy for diabetes mellitus and gene function analysis using a novel adenovirus vector
使用新型腺病毒载体进行糖尿病基因治疗和基因功能分析
  • 批准号:
    15K18939
  • 财政年份:
    2015
  • 资助金额:
    $ 23.64万
  • 项目类别:
    Grant-in-Aid for Young Scientists (B)
Innate immue response through glycolipids by adenovirus-vector
腺病毒载体通过糖脂产生先天免疫反应
  • 批准号:
    26450450
  • 财政年份:
    2014
  • 资助金额:
    $ 23.64万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Development of a novel method for highly efficient gene targeting by adenovirus vector on human naive pluripotent stem cells
开发一种通过腺病毒载体高效基因靶向人类幼稚多能干细胞的新方法
  • 批准号:
    26893253
  • 财政年份:
    2014
  • 资助金额:
    $ 23.64万
  • 项目类别:
    Grant-in-Aid for Research Activity Start-up
Development of targeting adenovirus vector as boron carrier for boron neutron capture therapy
开发靶向腺病毒载体作为硼中子捕获疗法的硼载体
  • 批准号:
    26462183
  • 财政年份:
    2014
  • 资助金额:
    $ 23.64万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Development of adenovirus vector lacking VA RNA genes for efficient microRNA expression
开发缺乏 VA RNA 基因的腺病毒载体以实现有效的 microRNA 表达
  • 批准号:
    24701021
  • 财政年份:
    2012
  • 资助金额:
    $ 23.64万
  • 项目类别:
    Grant-in-Aid for Young Scientists (B)
{{ showInfoDetail.title }}

作者:{{ showInfoDetail.author }}

知道了