The VA Gordon Mansfield SCI Consortium

 说明： 该项目旨在开发一种基于干细胞的疗法，以修复慢性损伤的脊髓，并最大限度地恢复功能。这项工作的成功开展可以将临床前观察从动物模型转化为临床实践。此外，我们的目标是开发创新的康复策略，以促进有意义的功能恢复，并客观地确定基于干细胞的治疗与康复相结合是否会优化功能结果。我们将专注于从已批准的胚胎干细胞系中获得的多潜能神经前体细胞，以及直接从成人成纤维细胞(一种诱导多潜能干细胞，IPSC)转分化而来的多潜能神经干细胞/祖细胞。这项工作将在啮齿动物和非人类灵长类物种中使用脊髓损伤的横断和挫伤模型。此外，我们建议由独立于该项目中的科学家联盟的研究人员进行重复研究。这是位于美国西海岸研究脊髓损伤的领先实验室之间的密切合作，这些实验室包括圣地亚哥退伍军人医学中心和加州大学圣地亚哥分校的Tuszynski小组；旧金山退伍军人医学中心和加州大学旧金山分校的Beattie/Bresnahan小组；加州大学洛杉矶分校的Edgerton小组；加州大学欧文分校的Havton和Rinkensmeyer小组；以及加州大学戴维斯分校的加州地区灵长类动物研究中心。拟议的实验是由这种合作共同设计和执行的，这是脊髓损伤研究中前所未有的努力，通过联合执行的实验的密切合作和互动来推动该领域的发展。在整个过程中，该项目将受益于合作者之间的信息自由交流。

 DESCRIPTION: The project aims to develop a stem cell-based therapy to repair the chronically injured spinal cord and to maximize functional recovery. Success development of this work can lead to translation of pre-clinical observations from animal models into clinical practice. Moreover, we aim to develop innovative rehabilitation strategies to promote meaningful recovery of function, and objectively determine whether a combination of stem cell-based therapies with rehabilitation will optimize functional outcomes. We will focus on multipotent neural progenitor cells derived from approved embryonic stem cell lines, and on multipotent neural stem /progenitor cells directly transdifferentiated from adult fibroblasts (a form of Induced Pluripotent Stem Cell, IPSC). The work will use both transection and contusion models of spinal cord injury in rodent and non-human primate species. Moreover, we propose replication studies by an investigator that is independent of the consortium of scientists in this project. This is an intensely collaborative effort among leading laboratories researching spinal cord injury located on the west coast of the United States, including the Tuszynski group at the San Diego VA Medical Center and UCSD; the Beattie/Bresnahan group at the San Francisco VA Medical Center and UCSF; the Edgerton group at UCLA; the Havton and Rinkensmeyer groups at UC Irvine; and the California Regional Primate Research Center located at UC Davis. Proposed experiments are collectively designed and performed by this collaboration, an unprecedented effort in spinal cord injury research to move the field forward through close collaboration and interaction on jointly performed experiments. Throughout, this project will benefit from a free exchange of information among the collaborators.