Core C RNA Sequencing and Bioinformatics

RNA测序已经迅速发展成为转录组学的革命性工具。目前，它是一个 评估转录丰度和变体的最敏感和最全面的工具 在各种生物样本中。在这一应用中，RNA-seq核心将在 对所有项目的样本进行转录组学和生物信息学分析。 RNA-SEQ/生物信息学核心的具体目标是： 1.为每个项目提供RNA测序(RNA-seq)服务。这包括 对聚A+RNA进行测序以定量组织中的多腺苷化转录本。 2.对RNA-SEQ数据进行生物信息学分析。这将包括相对的RNA 丰度、剪接变异和非人类开放阅读框的存在。 核心功能：将用一般术语描述核心功能，然后与 上面列出的具体目标。

RNA sequencing has quickly evolved to be a revolutionary tool for transcriptomics. Currently, it is one of the most sensitive and comprehensive tools for assessment of transcript abundance and variants in various biological samples. In this application, the RNA-seq Core will perform a vital role in performing transcriptomics and bioinformatic analyses of samples obtained from all projects. The specific aims of the RNA-seq/Bioinformatics Core are to: 1. Perform RNA sequencing (RNA-seq) services for each of the projects. This includes sequencing of poly A+ RNA to quantify polyadenylated transcripts in tissues. 2. Perform bioinformatic analysis of the RNA-seq data. This would include relative RNA abundance, splice variation, and presence of non-human ORFs. Core Function: The core functions will be described in general terms and then in relation to the specific objectives listed above.