Experience-dependent Modulation of Synaptic Circuits in the Hippocampus

海马突触回路的经验依赖性调节

• 批准号: 8873512
• 负责人: Juan Marcos Alarcon
• 金额: $ 25.54万
• 依托单位: SUNY DOWNSTATE MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-03-01 至 2017-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8873512
• 关键词: Active Learning; Animals; Area; Avoidance Learning; Behavioral; Brain; Cells; Cognition; Cognition Disorders; Cognitive; Control Groups; Detection; Dorsal; Exhibits; Failure; Goals; Hippocampus (Brain); Human; Image; Individual; Knowledge; Label; Laboratory mice; Lead; Learning; Measures; Memory; Messenger RNA; Mus; Neurons; Neurosciences; Pathway Analysis; Process; Property; Proteins; Reporting; Research; Semantics; Shock; Slice; Support System; Synapses; Synaptic plasticity; System; Tamoxifen; Testing; Time; Training; Transcript; Transgenic Mice; Work; entorhinal cortex; experience; extracellular; insight; mRNA Expression; neural circuit; novel; programs; promoter; protein expression; public health relevance; relating to nervous system; response; spatial memory; synaptic function

 DESCRIPTION (provided by applicant): Understanding how the brain changes with experience is a fundamental question in neuroscience. Memory systems are supported by multiple circuits that connect the neuronal ensembles that contain bits of information from particular learned experiences. We propose to investigate how synapses within the hippocampus circuit are changed by the persistent storage of a spatial memory. Our goal is to define the organization of memory traces at the level of synaptic circuits as part of a comprehensive research program to understand the processes and mechanisms of cognition, the malfunction of which may underlie cognitive disorders. We characterized the functional changes of hippocampal (CA3) and entorhinal cortical (EC layer III) synaptic inputs to CA1 neurons in mice that learned an active place avoidance task, a spatial learning task that depends on synaptic plasticity and its persistence in the dorsal hippocampus. Importantly, changes in hippocampal synaptic function persisted for at least 1 month after training and were observed only in animals in which the place avoidance memory also persisted. Changes in synaptic function did not occur in animals that were exposed to the behavioral arena (untrained control) or received delivery of unavoidable shocks (yoked control). Thus the changes in synaptic function were a consequence of the learning experience and were associated with the spatial memory itself. Interestingly, our observations indicated that these changes are rather large, probably reflecting an impact of learning experience on synaptic circuits beyond the few synapses that are inferred to store the specific bits of explicit information. This is a novel perspective; it seems that storing memory in a neural circuit involves broad changes in synaptic circuit function that may act as a supporting framework for the expression of the explicit information. We propose to use this robust experimental paradigm to further study how the storing of memories changes synaptic function within the hippocampal circuit. We seek to determine whether the persistent synaptic changes are localized to the subset of neurons that were active during the learning experience (Specific Aim 1) and to determine whether these changes parallel changes in the expression of mRNAs and proteins of known synaptic plasticity products (Specific Aim 2). Using this approach, we will direct our efforts to provide an analysis of how particular learned experiences persistently modify CA1 neurons at the level of CA3- CA1 and EC-CA1 synaptic inputs to gain insight toward the identification and organization of memory traces within synaptic circuits of the hippocampus. We hope to expand our understanding of how neural ensembles support memory at the systems level by investigating how learned information modulates the synaptic circuits that underlie the ensemble activity. As such, this scientific effort is expected to bridge the scales of the "synaptome" and the "connectome."

 描述（由申请人提供）：了解大脑如何随着经验而变化是神经科学中的一个基本问题。记忆系统由多个回路支持，这些回路连接包含来自特定学习经验的信息位的神经元集合。我们建议调查海马电路内的突触是如何改变持久存储的空间记忆。我们的目标是在突触回路的水平上定义记忆痕迹的组织，作为全面研究计划的一部分，以了解认知的过程和机制，其功能障碍可能是认知障碍的基础。我们的特点是海马（CA 3）和内嗅皮层（EC层III）突触输入到CA 1神经元的小鼠，学会了一个积极的地方回避任务，空间学习任务，依赖于突触可塑性和其持久性在背侧海马的功能变化。重要的是，海马突触功能的变化在训练后持续至少1个月，并且仅在位置回避记忆也持续存在的动物中观察到。在暴露于行为竞技场（未经训练的对照）或接受不可避免的电击（轭式对照）的动物中，突触功能没有发生变化。因此，突触功能的变化是学习经验的结果，并与空间记忆本身有关。有趣的是，我们的观察表明，这些变化相当大，可能反映了学习经验对突触回路的影响，而不仅仅是那些被推断为存储特定信息的突触。这是一个新颖的视角;看来，在神经回路中储存记忆涉及突触回路功能的广泛变化，这些突触回路功能可能充当表达显式信息的支持框架。 我们建议使用这个强大的实验范式，以进一步研究如何存储的记忆改变海马电路内的突触功能。我们试图确定持续的突触变化是否局限于学习过程中活跃的神经元子集（具体目标1），并确定这些变化是否与已知突触可塑性产物的mRNA和蛋白质表达的变化平行（具体目标2）。使用这种方法，我们将直接我们的努力，提供一个分析特定的学习经验如何持续修改CA 1神经元在CA 3-CA 1和EC-CA 1突触输入的水平，以深入了解对识别和组织的记忆痕迹内的海马突触回路。 我们希望通过研究学习信息如何调节作为整体活动基础的突触回路，来扩大我们对神经集合如何在系统水平上支持记忆的理解。因此，这一科学努力有望在“突触体”和“连接体”的尺度上架起桥梁。"