Proteomics Core

蛋白质组学核心

• 批准号: 8177749
• 负责人: Elizabeth Murphy
• 金额: $ 66.41万
• 依托单位: NATIONAL HEART, LUNG, AND BLOOD INSTITUTE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8177749
• 关键词: ADP ribosylation; Adrenal Cortex Hormones; Amino Acid Sequence; Apical; Asthma; Binding; Cell Line; Cell membrane; Cells; Consultations; Core Facility; Coupled; DNA-dependent protein kinase; Deacetylation; Dendritic Cells; Dictyostelium; Diet; Digestion; Disulfides; Drosophila genus; Duct (organ) structure; Epithelial; Exhalation; Extrinsic asthma; Family suidae; Fatty acid glycerol esters; Fractionation; Gel; Growth; Half-Life; Heart; Hepatic; High Density Lipoproteins; Human; Kidney; Low-Density Lipoproteins; Maps; Mass Spectrum Analysis; Mediating; Metabolism; Microtubules; Mitochondria; Modeling; Molecular; Mosses; Mus; Myoblasts; Myosin ATPase; Myosin Type II; N-acetyl-4-benzoquinoneimine; Organ; Oxidative Phosphorylation; Patients; Peptide Sequence Determination; Phosphatidylinositols; Phosphoproteins; Phosphoric Monoester Hydrolases; Phosphorylation; Phosphorylation Site; Phosphotransferases; Post-Translational Protein Processing; Preparation; Protein Isoforms; Protein Tyrosine Phosphatase; Proteins; Proteoglycan; Proteome; Proteomics; RNA Interference; Relative (related person); Reperfusion Injury; Research Personnel; Role; SKIL gene; STAT3 gene; Sampling; Services; Sex Characteristics; Signal Transduction; Sorting - Cell Movement; Specificity; Squamous cell carcinoma; Stem cells; Sulfur; Technology; Training; Translational Protein Modification; Transplantation; Vasopressins; WNT3 gene; adduct; adenylate kinase; base; cyclophilin D; genetic regulatory protein; mutant; nexin; non-muscle myosin; protein expression; protein profiling; response; tandem mass spectrometry; transcription factor

Investigator and Projects supported by the core: Adelstein Quantitation of the relative amounts of nonmuscle myosin II isoforms (II-A, II-B, and II-C) in various mammalian organs and cell lines Balaban Mitochondrial proteome; STAT3 quantitation in mitochondria; Oxidative phosphorylation in mitochondria; Proteoglycans binding to LDL Burg High NaCl-induced changes in phosphorylation of the osmoprotective transcription factor TonEBP Cannon Protein differences in isolated HDL between baseline and 9month excercise Chock Quantitative differences in protein expression using iTRAQ Chung Identification of DNA-PK interacting proteins; Identification of AMP-kinase phosphorylation sites in Rag-1 protein Epstein Interacting proteins using stem cells as the model Finkel The role of LSD1 in hepatic metabolism Geller Signaling mechanism of CSPG on axonal outgrowth inhibition (quantitative phosphoproteomics approach) Greene Identification of new binding partners for the molecular cochaperone, GAK Hwang Identification of proteins in BN-gels of human myoblast mitochondria Ito Studies on sulphur-rich proteins at the growing edge of transplanted mouse squamous cell carcinoma Knepper Kinase specificity profiling using LC-MS/MS; Global profiling of protein half-lives in cultured collecting duct cells using SILAC; iTRAQ-based quantitation of vasopressin-regulated phosphoproteins; Quantitative analysis of apical plasma membrane in mpkCCD cells in response to vasopressin using SILAC Korn Identification of tyrosine phosphatase from Dictyostelium Levine S Protein identification in exhaled breath condensate in patients with asthma; Proteomic profiling of corticosteroid responsive and non-responsive proteins in murine model of allergic asthma; Proteomic profiling of exosomes derived from immature dendritic cells Manganiello Identification of mitochondrial signalosome and differences between WT and PDE3B KO heart after ischemia/reperfusion injury Milgram Interacting proteins with sorting nexin 27 in kidney epithelial lysate Moss Characterization of ADP ribosylation; Incorporation of 18O during ARH3 deacetylation of OAADPr Murphy Identification of cyclophilin D binding partners; Quantitation of VDAC phosphorylation; SNO quantitation by ICAT and SNO-RAC; Gender differences in mouse mitochondria Peterkofsky Identification of Ecoli proteins using in-gel digestion Puertollano Characterization of a phosphatidylinositol-4, 5-biphosphate 4-phosphatase Remaley Proteomic differences as induced by high fat diet Sack Proteomic differences in STAT3 KO mice; MAT1 acetylome; Identification of Pink1 interacting proteins; Identification of NAPQI adduct in ALDH2 Sellers Identification of Drosophila myosin 7a activating protein (DMAP) Tjandra Disulfide mapping of WNT3 protein from Galus Galus Young Relative quantitation of GPI-anchored proteins in mutant PIG-A cell lines Waterman An RNAi screen of microtubuler-regulatory proteins identifies MARK2/Par1 as an effector of Rac1-mediated microtubule growth

核心支持的调查员和项目： 阿德尔斯坦 不同哺乳动物器官和细胞系中非肌肉肌球蛋白II亚型(II-A、II-B和II-C)相对含量的测定 巴拉班 线粒体蛋白质组；线粒体STAT3定量；线粒体氧化磷酸化；蛋白多糖与低密度脂蛋白结合 伯格 高盐诱导渗透保护转录因子TONEBP磷酸化的变化 大炮 基线运动与9个月运动时高密度脂蛋白的蛋白质差异 卡住 利用iTRAQ技术研究蛋白质表达的定量差异 钟 DNA-PK相互作用蛋白的鉴定及RAG-1蛋白中AMP-K磷酸化位点的鉴定 爱泼斯坦 以干细胞为模型的相互作用蛋白质 芬克尔 LSD1在肝脏代谢中的作用 盖勒 CSPG抑制轴突生长的信号机制(定量磷酸蛋白质组学方法) 格林 分子伴侣GAK的新结合伙伴的鉴定 黄哲伦 人成肌细胞线粒体BN凝胶中蛋白质的鉴定 伊藤 小鼠移植性鳞癌生长边缘富硫蛋白的研究 克内珀 利用LC-MS/MS技术分析蛋白激酶的特异性；使用SILAC技术对培养的集合管细胞中的蛋白质半衰期进行整体分析；基于iTRAQ的血管加压素调节的磷蛋白的定量研究；使用SILAC技术对mpkCD细胞顶端质膜对血管加压素的响应进行定量分析。 科恩 网柄苔藓中酪氨酸磷酸酶的鉴定 莱文·S 哮喘患者呼气冷凝液中蛋白质的鉴定；过敏性哮喘小鼠模型中糖皮质激素反应性和非反应性蛋白质的蛋白质图谱；未成熟树突状细胞来源的外切体的蛋白质图谱 曼加尼洛 WT和PDE3B KO心脏缺血/再灌注损伤后线粒体信号体的鉴定及差异 米尔格拉姆 肾上皮细胞裂解液中与分类连接蛋白27相互作用的蛋白 苔藓 ADP核糖化反应的表征：OAADPr在ARH3脱乙酰化过程中18O的掺入 墨菲 亲环素D结合伙伴的鉴定；VDAC磷酸化的定量；SNO的ICAT和SNO-RAC定量；小鼠线粒体的性别差异 彼得科夫斯基 应用凝胶内消化技术鉴定大肠杆菌蛋白质 普尔托拉诺 磷脂酰肌醇-4，5-二磷酸-4-磷酸酶的性质 雷梅利 高脂饮食诱导的蛋白质组学差异 麻袋 STAT3 KO小鼠蛋白质组差异；MAT1乙酰组；PINK1相互作用蛋白的鉴定；ALDH2中NAPQI加合物的鉴定 卖家 果蝇肌球蛋白7a激活蛋白(DMAP)的鉴定 Tjandra 银河猴WNT3蛋白的二硫键定位 年青的 突变的PIG-A细胞系中GPI锚定蛋白的相对定量 沃特曼 微管调节蛋白的RNAi筛选表明Mark2/Par1是rac1介导的微管生长的效应因子