Proteomics Core

蛋白质组学核心

• 批准号: 8558132
• 负责人: Elizabeth Murphy
• 金额: $ 148.44万
• 依托单位: NATIONAL HEART, LUNG, AND BLOOD INSTITUTE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8558132
• 关键词: ADP ribosylation; Acetylation; Acids; Affinity; Amino Acid Sequence; Apical; Apolipoprotein A-I; Binding; Biogenesis; Cell Line; Cell membrane; Cells; Complex; Consultations; Core Facility; Coupled; Dendritic Cells; Duct (organ) structure; Epithelial; Focal Adhesions; Fractionation; Gel; Halothane; Heart; Heat-Shock Proteins 90; Intake; Ischemia; Ischemic Preconditioning; Kidney; Kinetics; Lipolysis; Liver; Low-Density Lipoproteins; Lung; Lysosomes; Mass Spectrum Analysis; Measurement; Membrane; Mitochondria; Mitochondrial Proteins; Modification; Mosses; Mus; Myosin Type II; National Heart, Lung, and Blood Institute; Organ; Ovalbumin; Peptide Sequence Determination; Peptides; Phosphoproteins; Phosphorylation; Phosphorylation Site; Phosphotransferases; Post-Translational Protein Processing; Preparation; Protein Binding; Protein Isoforms; Protein Secretion; Proteins; Proteome; Proteomics; Relative (related person); Reperfusion Injury; Reperfusion Therapy; Research Personnel; Role; SKIL gene; Sampling; Serine; Services; Sickle Cell; Signal Transduction; Sorting - Cell Movement; Specificity; TNFRSF1A gene; Technology; Training; Ubiquitination; Vasopressins; analog; base; cyclophilin D; induced pluripotent stem cell; messenger ribonucleoprotein; methionine sulfoxide; nexin; nitration; non-muscle myosin; novel; protein profiling; response; tandem mass spectrometry; trafficking; transcription factor

NHLBI Investigators and their projects Adelstein Quantitation of the relative amounts of nonmuscle myosin II isoforms (II-A, II-B, and II-C) in various mammalian organs and cell lines; Characterization of the role of NMM II isoforms in focal adhesions and identifying novel binding partners to NMM II isoforms Anderson Phosphorylation of Claudin-2 on serine 208 promotes membrane retention and reduces trafficking to lysosomes Balaban Mitochondrial proteome; Acid-labile phosphorylation; Mitochondrial protein panel Burg High NaCl-induced changes in phosphorylation of the osmoprotective transcription factor TonEBP; Peptide affinity to identify proteins that bind to NFAT5 Cannon Nitration of ApoA-I Cao Lipolysis induced protein secretion Chen (iPSC Core) Seleneproteomics Chung Identification of phosphorylation sites on HSP90 Donaldson Proteins associated with endocytic cargo proteins Finkel Targeted acetylation on RIP1 Geller Signaling mechanism of CSPG on axonal outgrowth inhibition (quantitative phosphoproteomics approach) Hogg Protein identification and relative quantification in affinity purified mRNP complexes Hwang Proteomic differences in mitochondria from p53 KO mice Kato Sickle cell proteomics Knepper Kinase specificity profiling using LC-MS/MS; Global profiling of protein half-lives in cultured collecting duct cells using SILAC; iTRAQ-based quantitation of vasopressin-regulated phosphoproteins; Quantitative analysis of apical plasma membrane in mpkCCD cells in response to vasopressin using SILAC; SUMOylation differences after vasopressin treatment Levine S Proteomic profiling of exosomes derived from immature dendritic cells; Differential proteomics in lungs of ovalbumin-challenged asthmatic mice; Kinetics of ADAM9 and TACE cleavage in TNFR1 shedding Levine R Identification of methionine sulfoxide modification Manganiello Identification of mitochondrial signalosome and differences between WT and PDE3B KO heart after ischemia/reperfusion injury Milgram Interacting proteins with sorting nexin 27 in kidney epithelial lysate Moss Characterization of ADP ribosylation; Identification of ARH1 target proteins Murphy Identification of cyclophilin D binding partners; SNO quantitation SNO-RAC; Acetylation and ubiquitination differences as a consequence of ischemia/reperfusion or ischemic preconditioning; Nitrosylation occupancy measurement by using cys-TMT Pohl Relative protein differences in halothane treated liver Puertollano De-phosphorylation of TFEB in lysosomal biogenesis Remaley Peptide 5A analogs and its intake by LDL Rusan Plk4 kinase partners and activity; PLP binding partners; Centrosomal protein and PTMs identification Sack Identification of Pink1 interacting proteins; Acetylation in SIRT3 and SIRT5 KO mice; Ubiquitination; Targeted lipidomics Zhao KD of PAKs and its effect on H4 phosphorylation

NHLBI调查人员及其项目 阿德尔斯坦 定量不同哺乳动物器官和细胞系中非肌肉肌球蛋白II亚型(II-A、II-B和II-C)的相对数量；表征NMM II亚型在局灶性粘连中的作用并确定与NMM II亚型新的结合伙伴 安德森 丝氨酸208上Claudin-2的磷酸化促进膜保留并减少向溶酶体的转运 巴拉班 线粒体蛋白质组；酸不稳定磷酸化；线粒体蛋白质组 伯格 高盐诱导渗透保护性转录因子TONEBP的磷酸化变化；多肽亲和力鉴定与NFAT5结合的蛋白质 大炮 载脂蛋白A-I的硝化反应 曹氏 脂肪分解诱导的蛋白质分泌 陈(IPSC酷睿) 硒蛋白质组学 钟 热休克蛋白90上磷酸化位点的鉴定 唐纳森 与胞内货物蛋白相关的蛋白质 芬克尔 RIP1上的靶向乙酰化 盖勒 CSPG抑制轴突生长的信号机制(定量磷酸蛋白质组学方法) 霍格 亲和纯化mRNP复合体的蛋白质鉴定和相对定量 黄哲伦 P53 KO小鼠线粒体蛋白质组学差异研究 加藤 镰状细胞蛋白质组学 克内珀 利用LC-MS/MS技术分析蛋白激酶的特异性；使用SILAC技术对培养的集合管细胞中的蛋白质半衰期进行整体分析；基于iTRAQ的血管加压素调节的磷蛋白的定量研究；使用SILAC技术对mpkCD细胞顶端质膜对加压素的反应进行定量分析；在加压素处理后的SUMO化差异。 莱文·S 未成熟树突状细胞外切体的蛋白质组学研究；卵蛋白激发哮喘小鼠肺内差异蛋白质组学；肿瘤坏死因子受体1脱落过程中ADAM9和TACE的裂解动力学 莱文R 甲硫氨酸亚砜的改性鉴定 曼加尼洛 WT和PDE3B KO心脏缺血/再灌注损伤后线粒体信号体的鉴定及差异 米尔格拉姆 肾上皮细胞裂解液中与分类连接蛋白27相互作用的蛋白 苔藓 ADP核糖化的鉴定及ARH1靶蛋白的鉴定 墨菲 亲环素D结合伙伴的鉴定；SNO定量SNO-RAC；缺血/再灌注或缺血预适应引起的乙酰化和泛素化差异；用CyS-TMT测定亚硝酸化占有率 Pohl 氟烷处理肝脏中相关蛋白质的差异 普尔托拉诺 TFEB在溶酶体生物发生中的去磷酸化 雷梅利 肽5A类似物及其对低密度脂蛋白的摄取 乳山 PLK4蛋白和活性；PLP结合蛋白；中心体蛋白和PTMS鉴定 麻袋 PINK1相互作用蛋白的鉴定；SIRT3和SIRT5 KO小鼠的乙酰化；泛素化；靶向脂质组学 赵某 PAKs的Kd及其对H4磷酸化的影响