TAS::75 0862::TAS

塔斯马尼亚州::75 0862::塔斯马尼亚州

• 批准号: 8164000
• 负责人: MARTIN D. CHAPMAN
• 金额: $ 14.93万
• 依托单位: INDOOR BIOTECHNOLOGIES
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-09-28 至 2011-09-27
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8164000
• 关键词: Alabama; Allergens; Allergic Disease; Alternaria; Antibodies; Antigens; Arts; Aspergillus; Aspergillus fumigatus; Asthma; Biological Assay; Biological Markers; Biotechnology; Birds; Calibration; Canada; Centers for Disease Control and Prevention (U.S.); Chronic Fatigue Syndrome; Cities; Client; DNA; Detection; Development; Dust; Environmental Exposure; Epidemiologic Studies; Evaluation; Exposure to; Extrinsic asthma; Government Agencies; Health; House Dust; Housing; Immune response; Indoor Air Quality; Industry; Institution; Investigation; Kansas; Lung diseases; Measures; Methods; Migraine; Missouri; Molds; Monoclonal Antibodies; National Institute of Environmental Health Sciences; Penicillium; Phase; Polystyrenes; Reproducibility; Research; Role; Sales; Sampling; Sensitivity and Specificity; Services; Sinusitis; Stachybotrys; System; Technology; Testing; Validation; Water; cross reactivity; innovation; instrumentation; interest; phase 1 study; phase 2 study; population survey

Molds cause asthma and allergic respiratory diseases and are associated with other illnesses such as sinusitis, chronic fatigue syndrome, lethargy, or migraines. Mold occurs in damp or water damaged housing. The relationships between mold exposure and health effects are poorly understood. The aim of this project is to develop a multiplex array for mold biomarkers that will measure 4-7 biomarkers, simultaneously, from molds associated with asthma or that are found in water damaged buildings. The Technical Objectives are i) to develop a fluorescent Multiplex Array for Mold Biomarkers (MAMB) on the xMAP system to measure allergens/antigens from Alternaria, Aspergillus fumigatus, A. versicolor, Stachybotrys and Penicillium spp.; ii) to determine the sensitivity, specificity, reproducibility of the MAMB; and iii) to use the MAMB to measure mold exposure in dust samples from environmental studies of damp housing in the U.S. and Canada. The MAMB will use monoclonal antibody pairs for allergen capture and detection and a standard of purified biomarkers for calibration purposes. Current methods for mold testing, such as mold counts or DNA analyses, do not measure specific biomarkers. The MAMB will apply innovative, state-of-the art array technology to assess exposure to specific mold antigens, some of which are known to cause deleterious immune responses. The array for up to 7 biomarkers is expandable to include other mold antigens, as part of Phase II studies. The MAMB will provide quantiative measures of mold biomarker exposure in a single test and will facilitate large scale epidemiologic studies and population surveys on the health effects of environmental exposure to molds.

霉菌会引起哮喘和过敏性呼吸道疾病，并与其他疾病有关， 鼻窦炎、慢性疲劳综合征、嗜睡或偏头痛。霉菌发生在潮湿或水损坏的外壳中。 霉菌暴露和健康影响之间的关系知之甚少。这个项目的目的 是开发一种用于霉菌生物标志物的多重阵列，该阵列将同时测量4-7种生物标志物， 与哮喘有关的霉菌或在水损坏的建筑物中发现的霉菌。技术目标：i） 在xMAP系统上开发用于霉菌生物标志物（MAMB）的荧光多重阵列， 来自链格孢属、烟曲霉、A. versicolor、Stachybocytes和青霉属; ii）第二阶段 确定MAMB的灵敏度、特异性、再现性;和iii）使用MAMB测量 在美国和加拿大潮湿房屋的环境研究中，灰尘样本中的霉菌暴露。的 MAMB将使用单克隆抗体对进行过敏原捕获和检测，并使用纯化的标准品。 用于校准目的的生物标志物。目前的霉菌检测方法，如霉菌计数或DNA 分析，不测量特定的生物标志物。MAMB将采用创新的、最先进的阵列 技术来评估暴露于特定的霉菌抗原，其中一些已知会导致有害的 免疫反应。多达7种生物标志物的阵列可扩展以包括其他霉菌抗原，作为 II期研究。MAMB将在单一测试中提供霉菌生物标志物暴露的定量测量 并将促进大规模的流行病学研究和人口调查的健康影响， 环境暴露于霉菌。