TAS::75 0849::TAS DEVELOPMENT A PROPRIETARY TIP-ENHANCED RAMAN SPECTROSCOPY (TER

TAS::75 0849::TAS 开发专有的尖端增强拉曼光谱仪（TER

• 批准号: 8171205
• 负责人: DAVID WOLF
• 金额: $ 15万
• 依托单位: RADIATION MONITORING DEVICES, INC.
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-09-24 至 2011-05-23
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8171205
• 关键词: 8-hydroxyguanosine; Biological Assay; Cancer Etiology; DNA; DNA Sequence; Development; Gene Mutation; Lesion; Malignant Neoplasms; Methods; Methylation; Modality; Modification; Nucleotides; RNA; Raman Spectrum Analysis; Single-Stranded DNA; Techniques; Technology; base; novel; single molecule

Mutation of genetic DNA is a major factor causing cancer. Locating these changes in the DNA sequence of single molecules is difficult because they cannot be assayed by conventional sequencing techniques. This project will demonstrate sequencing capabilities for single stranded DNA and RNA immobilized on a novel substrate, and demonstrate a novel method for increasing throughput. Sensitivity to mid-sequence lesions and base modifications such as 8-oxoguanosine, base methylation, and other cancer-relevant nucleotide modifications will also be demonstrated.

基因DNA突变是导致癌症的主要因素。在单个分子的DNA序列中定位这些变化是困难的，因为它们不能通过常规测序技术进行测定。该项目将展示固定在新型底物上的单链DNA和RNA的测序能力，并展示提高通量的新方法。还将证明对中间序列病变和碱基修饰（如8-氧代鸟苷、碱基甲基化和其他癌症相关核苷酸修饰）的敏感性。