Epigenetic landscapes of embryonic lymphoid progenitors and HSCs

胚胎淋巴祖细胞和 HSC 的表观遗传景观

基本信息

批准号：
8891754
负责人：
NANCY SPECK
金额：
$ 20.92万
依托单位：
UNIVERSITY OF PENNSYLVANIA
依托单位国家：
美国
项目类别：
财政年份：
2015
资助国家：
美国
起止时间：
2015-04-25 至 2017-03-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8891754
关键词：
Adult Algorithms Aorta Bioinformatics Biological Assay Blood Cells Blood Circulation Bone Marrow Bone Marrow Transplantation Cell Count Cell Maturation Cell surface Cells ChIP-seq Collaborations Data Development Embryo Endothelial Cells Endothelium Enhancers Epigenetic Process Erythroid Fetal Liver Gene Expression Gene Expression Profile Gene Targeting Genes Goals Gonadal structure Hematological Disease Hematopoietic Hematopoietic stem cells Immune In Vitro Joints Life Link Lymphoid Malignant - descriptor Mesonephric structure Methods Molecular Monitor Myelogenous Non-Malignant Pathway interactions Patients Population Problem Solving Procedures Production Regenerative Medicine Signal Pathway Site Specific qualifier value Staging Technology Testing base chromatin modification differential expression epigenome epigenomics factor A fetal genome-wide histone modification induced pluripotent stem cell innovation molecular marker progenitor public health relevance research study tissue culture transcriptome sequencing transcriptomics

项目摘要

DESCRIPTION (provided by applicant): An important objective in regenerative medicine is to make hematopoietic stem cells (HSCs) from embryonic or induced pluripotent stem cells (ESCs or iPSCs). HSCs differentiate from a small population of endothelial cells in the embryo called hemogenic endothelium. The differentiation of HSCs from hemogenic endothelium involves an intermediate step in which blood cells accumulate in the major vasculature as pre-HSCs before being released into the circulation to colonize the fetal liver, where the pre-HSCs mature into fully functional HSCs. We plan to profile the gene expression and epigenetic changes that occur during the transitions between embryonic pre-HSCs, an intermediate population that we call "HSC explant" (HSCex), and fetal liver HSCs. We will also analyze differentially expressed cell surface markers for their utility in purifying embryonic HSCs. The project involves collaboration between a developmental biologist and a bioinformatician.

描述（由适用提供）：再生医学的一个重要目标是从胚胎或诱导多能干细胞（ESC或IPSC）中制造造血干细胞（HSC）。 HSC与称为血液内皮的胚胎中的少量内皮细胞分化。 HSC与血肿内皮的分化涉及一个中间步骤，在该步骤中，血细胞在主要脉管系统中积聚为前HSC，然后释放到循环中以定居胎儿肝脏，其中HSC将HSC成熟到功能齐全的HSC中。我们计划介绍胚胎前HSC之间的过渡期间发生的基因表达和表观遗传变化，这是我们称为“ HSC Epplant”（HSCEX）和胎儿肝HSC的中间种群。我们还将分析不同表达的细胞表面标记，以纯化纯化胚胎HSC。该项目涉及发展生物学家与生物信息学家之间的合作。