Epigenetic landscapes of embryonic lymphoid progenitors and HSCs

胚胎淋巴祖细胞和 HSC 的表观遗传景观

• 批准号: 8891754
• 负责人: NANCY SPECK
• 金额: $ 20.92万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-04-25 至 2017-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8891754
• 关键词: Adult; Algorithms; Aorta; Bioinformatics; Biological Assay; Blood Cells; Blood Circulation; Bone Marrow; Bone Marrow Transplantation; Cell Count; Cell Maturation; Cell surface; Cells; ChIP-seq; Collaborations; Data; Development; Embryo; Endothelial Cells; Endothelium; Enhancers; Epigenetic Process; Erythroid; Fetal Liver; Gene Expression; Gene Expression Profile; Gene Targeting; Genes; Goals; Gonadal structure; Hematological Disease; Hematopoietic; Hematopoietic stem cells; Immune; In Vitro; Joints; Life; Link; Lymphoid; Malignant - descriptor; Mesonephric structure; Methods; Molecular; Monitor; Myelogenous; Non-Malignant; Pathway interactions; Patients; Population; Problem Solving; Procedures; Production; Regenerative Medicine; Signal Pathway; Site; Specific qualifier value; Staging; Technology; Testing; base; chromatin modification; differential expression; epigenome; epigenomics; factor A; fetal; genome-wide; histone modification; induced pluripotent stem cell; innovation; molecular marker; progenitor; public health relevance; research study; tissue culture; transcriptome sequencing; transcriptomics

 DESCRIPTION (provided by applicant): An important objective in regenerative medicine is to make hematopoietic stem cells (HSCs) from embryonic or induced pluripotent stem cells (ESCs or iPSCs). HSCs differentiate from a small population of endothelial cells in the embryo called hemogenic endothelium. The differentiation of HSCs from hemogenic endothelium involves an intermediate step in which blood cells accumulate in the major vasculature as pre-HSCs before being released into the circulation to colonize the fetal liver, where the pre-HSCs mature into fully functional HSCs. We plan to profile the gene expression and epigenetic changes that occur during the transitions between embryonic pre-HSCs, an intermediate population that we call "HSC explant" (HSCex), and fetal liver HSCs. We will also analyze differentially expressed cell surface markers for their utility in purifying embryonic HSCs. The project involves collaboration between a developmental biologist and a bioinformatician.

 描述（由申请人提供）：再生医学的一个重要目标是从胚胎或诱导多能干细胞（ESC或iPSC）制备造血干细胞（HSC）。HSC从胚胎中称为生血内皮的一小群内皮细胞分化而来。HSC从生血内皮分化涉及中间步骤，其中血细胞在释放到循环中以定殖胎肝之前作为前HSC在主要脉管系统中积累，其中前HSC成熟为完全功能的HSC。我们计划分析胚胎前HSC（我们称之为“HSC外植体”（HSCex）的中间群体）和胎肝HSC之间过渡期间发生的基因表达和表观遗传变化。我们还将分析差异表达的细胞表面标记物在纯化胚胎HSC中的效用。该项目涉及发展生物学家和生物信息学家之间的合作。