Thermodynamics and kinetics of DNA-ligand binding probed by DNA overstretching

DNA 过度拉伸探测 DNA-配体结合的热力学和动力学

• 批准号: 9207510
• 负责人: Andreas Hanke
• 金额: $ 10.16万
• 依托单位: UNIVERSITY OF TEXAS RIO GRANDE VALLEY
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-05-01 至 2017-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9207510
• 关键词: Thermodynamics; kinetics; DNA; ligand; binding

DESCRIPTION (provided by applicant): Structural transitions of DNA and docking of DNA-binding ligands on DNA are fundamental processes in molecular biology. Understanding and controlling these processes are essential to the rational design of novel drugs against cancer and infectious diseases such as HIV. Single-molecule force measurements using optical and magnetic tweezers and atomic force microscopy have dramatically expanded our knowledge of nucleic acids and proteins. Specifically, stretching single DNA molecules by an optical tweezers instrument can induce the unwinding of the two strands of the DNA duplex. The induced structural and thermodynamic changes in the DNA double helix upon stretching alter interactions with DNA-binding ligands in a controllable and measurable way. Therefore single-molecule force measurements of DNA and DNA-ligand interactions provide an unprecedented opportunity for quantitative study of a wide range of physiologically important phenomena associated with DNA helix-destabilization and DNA-ligand binding. In spite of the progress made in single-molecule force experiments, a poor understanding of the structural and thermodynamic response of biomolecules to mechanical stress has limited the insight that such experiments have provided into helix destabilization and DNA-ligand binding. A notorious difficulty for modeling force-induced melting is the vast range of length and time scales spanned by the process, and by the difficulty in computing the accompanying change in entropy. To better understand the fundamental biophysics involved in crossing length and times scales from the atomistic to the macromolecular model, we develop a novel multiscale model for DNA stretching and small ligand binding to stretched DNA. This system is well characterized experimentally and sufficiently simple to facilitate chemically accurate biophysical modeling in terms of all-atom molecular dynamics (MD) simulations and coarse-grained models such as the Poland-Scheraga model. Moreover, DNA stretching in the presence of DNA binding ligands yields information about DNA ligand binding under realistic, biologically relevant conditions that cannot be obtained by other methods. In this project we study the binding of Actinomycin D (ActD), an anticancer antibiotic drug that targets DNA replication and transcription, by a multipronged approach. On the atomistic scale, chemically accurate MD simulations will be used to study the binding mechanism and dynamics of ActD to short DNA oligomers; for long DNA as used in stretching experiments, our multiscale model will be used to compute equilibrium force-extension relations that can be directly validated with experimental data. The project will both enhance our knowledge of multiscale phenomena in fundamental biophysics and provide new insight into the anticancer function of ActD.

描述（由申请人提供）：DNA的结构转换和DNA结合配体在DNA上的对接是分子生物学中的基本过程。理解和控制这些过程对于合理设计对抗癌症和艾滋病等传染病的新药至关重要。利用光镊、磁镊和原子力显微镜进行的单分子力测量极大地扩展了我们对核酸和蛋白质的认识。具体地，通过光镊仪器拉伸单个DNA分子可以诱导DNA双链体的两条链的解旋。拉伸后DNA双螺旋中诱导的结构和热力学变化以可控和可测量的方式改变与DNA结合配体的相互作用。因此，DNA和DNA-配体相互作用的单分子力测量为定量研究与DNA螺旋不稳定和DNA-配体结合相关的广泛的生理学重要现象提供了前所未有的机会。 尽管在单分子力实验中取得了进展，但对生物分子对机械应力的结构和热力学响应的理解不足，限制了此类实验提供的螺旋不稳定和DNA-配体结合的见解。建模力诱导熔化的一个众所周知的困难是该过程跨越的长度和时间尺度的巨大范围，以及计算伴随的熵变化的困难。 为了更好地理解基本的生物物理学涉及的交叉长度和时间尺度从原子到大分子模型，我们开发了一种新的多尺度模型的DNA拉伸和小配体结合拉伸DNA。该系统的特点是实验和足够简单，以促进化学准确的生物物理建模方面的全原子分子动力学（MD）模拟和粗粒度的模型，如波兰-Scheraga模型。此外，在DNA结合配体存在下的DNA拉伸产生关于在现实的、生物学相关的条件下DNA配体结合的信息，这是不能通过其他方法获得的。在这个项目中，我们研究结合放线菌素D（ActD），一种抗癌抗生素药物，靶向DNA复制和转录，通过多管齐下的方法。在原子尺度上，化学精确的MD模拟将用于研究ActD与短DNA寡聚体的结合机制和动力学;对于拉伸实验中使用的长DNA，我们的多尺度模型将用于计算平衡力-延伸关系，该关系可以直接用实验数据进行验证。该项目将增强我们对基础生物物理学中多尺度现象的认识，并为ActD的抗癌功能提供新的见解。