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Animal Models and Tissue Engineering Core

动物模型和组织工程核心

基本信息

批准号：
8900965
负责人：
Howard Levinson
金额：
$ 24.81万
依托单位：
DUKE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
资助国家：
美国
起止时间：
至
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/8900965
关键词：
3-Dimensional Adipose tissue Adult Alleles Animal Model Antibodies Appearance Architecture Basic Science Biochemistry Biological Biology Blood Circulation Cells Chemicals Chimeric Proteins Chronic Cicatrix Communities Complex Consultations Cultured Cells Cutaneous Data Analyses Dermal Dermatologist Development Diagnostic Differentiation and Growth Disease Education Embryo Engineered Gene Epidermis Epithelial Experimental Designs Family suidae Fibroblasts Frequencies Gene Delivery Generations Genes Genetic Genetic Engineering Goals Granulation Tissue Hair follicle structure Heating Human Hypertrophic Cicatrix Immune Immunodeficient Mouse Inflammation Intravenous Investigation Knock-out Life Mechanics Medical Modeling Mus Mutation Natural regeneration Neurons Newborn Infant Nucleic Acids Operative Surgical Procedures Oral Organ Outcome Measure Pathogenesis Pathology Patients Peritoneal Permeability Pharmaceutical Preparations Pharmacologic Substance Physiological Physiology Production Proteins Protocols documentation Pump Research Research Design Research Personnel Resources Rodent SCID Mice Services Skin Skin Carcinogenesis Skin Tissue Staging Surface Surgeon System Techniques Therapeutic Agents Thick Tissue Engineering Tissue Model Tissues Translational Research Transplantation Virus Work Wound Healing Xenograft procedure angiogenesis animal model selection animal tissue base cell type clinically relevant density empowered human tissue image guided insight interest keratinocyte knockout gene melanocyte member morphometry mouse model pre-clinical pre-clinical research programs skin disorder skin regeneration subcutaneous success three dimensional structure tool wound

项目摘要

Animal Models and Tissue Engineering Core The skin is a complex organ that undergoes life-long renewal through spatially programmed growth and differentiation. It is composed of many distinct cell types (epithelial, fibroblast, endothelial, adipose, immune, neurons, etc.) that work in concert to allow normal physiology or that are disrupted to yield pathologies. Both normal and disease states of the skin are characterized by a specific three-dimensional architecture and interactions between these cell types which cultured cells in isolation cannot fully recapitulate. A critical aspect of basic and translational research involves the generation and application of skin tissue and animal models with an intact epidermal permeability barrier and physiologic connections to the systemic circulation. The goal of the Animal Models and Tissue Engineering Core is to provide consultation, generation and data analyses of a broad range of animal and skin tissue models. These include 1) 3-D organotypic skin culture, 2) de novo human skin regeneration on immunodeficient mice with genetically modified human epidermal and dermal cells, 3) lesional or normal human skin xenotransplantation onto immunodeficient mice, 4) various surgical and physiological wound healing models of mouse and swine, and 5) mouse models with skin cell targeted genetic engineering. These models will be tailored towards the specific needs of each investigator for functional, mechanistic and pharmacological studies. Our service will be provided through team efforts of basic science researchers, dermatologists and surgeons. These core resources will not only strengthen the investigations of current skin but also facilitate entry into skin research for other investigators who lack the technical basis to initiate such effort given a historical focus in other systems. We are confident that Duke research community has a strong desire and an enormous capacity to expand its skin disease-focused research.

动物模型与组织工程核心皮肤是一个复杂的器官，通过空间程序化的生长和分化经历终身更新。它由许多不同的细胞类型（上皮、成纤维细胞、内皮、脂肪、免疫、神经元等）组成。它们协同工作以允许正常的生理学或被破坏以产生病理学。皮肤的正常状态和疾病状态的特征在于特定的三维结构以及这些细胞类型之间的相互作用，这些细胞类型在隔离中培养的细胞不能完全重现。基础和转化研究的一个关键方面涉及皮肤组织和动物模型的生成和应用，这些模型具有完整的表皮渗透屏障和与体循环的生理连接。动物模型和组织工程核心的目标是提供广泛的动物和皮肤组织模型的咨询，生成和数据分析。这些包括1）3-D器官型皮肤培养， 2)用遗传修饰的人表皮和真皮细胞在免疫缺陷小鼠上从头人皮肤再生，3）将损伤或正常人皮肤异种移植到免疫缺陷小鼠上，4）小鼠和猪的各种手术和生理伤口愈合模型，和5）用皮肤细胞靶向遗传工程的小鼠模型。这些模型将根据每位研究者的功能、机制和药理学研究的具体需求进行定制。我们的服务将通过基础科学研究人员，皮肤科医生和外科医生的团队努力提供。这些核心资源不仅将加强对当前皮肤的研究，而且还将促进缺乏技术基础的其他研究人员进入皮肤研究，因为这些研究人员在其他系统中具有历史重点。我们相信，杜克研究界有强烈的愿望和巨大的能力，以扩大其皮肤病为重点的研究。