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Regulation of biofilm formation and pathogenesis in Bordetella pertussis

Regulation of biofilm formation and pathogenesis in Bordetella pertussis
百日咳博德特氏菌生物膜形成和发病机制的调控
批准号:
9092038
负责人:
RAJENDAR K DEORA
金额:
$19.38万
依托单位国家:
美国
项目类别:
财政年份:
2016
资助国家:
美国
项目状态:
已结题
起止时间:
2016-04-20 至 2018-03-31

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中文摘要
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英文摘要
 DESCRIPTION (provided by applicant): The Gram-negative bacterium Bordetella pertussis is a human restricted pathogen that causes severe respiratory infection and the disease known as whooping cough or pertussis. The recent rise in B. pertussis infections and the disease in the USA and other high income countries, along with waning immunity to current commercial pertussis vaccines necessitate research on understanding the pathogenic mechanisms employed by this bacterium. Our overall hypothesis is that the formation of biofilms is essential for persistent colonization of the respiratory tract and pathogenesis of B. pertussis. Control of biofilm development in the largely animal pathogen B. bronchiseptica is mediated by BpsR, a highly conserved transcriptional regulator. Additionally, BpsR dually activates or represses the expression of a large number of genes which have roles in virulence, transcriptional regulation, metabolic and other cellular processes. Nothing is known about the role of BpsR in B. pertussis. Functional studies of BpsR in B. pertussis have been hindered by the inability to construct a mutant strain lacking the bpsR gene. This suggests that bpsR is an essential gene in B. pertussis. We hypothesize that by regulating the expression of genes involved in essential metabolic pathways, pathogenesis and biofilm formation, BpsR controls the ability of B. pertussis to survive in the respiratory tract. In Specific Aim 1, we will utilize a tetracycline dependent conditional silencing system to examine its role in laboratory growth, gene expression and biofilm development. In Specific Aim 2, these studies will be extended to an intranasal mouse model of B. pertussis infection and biofilm development to identify its role in colonization of and formation of biofilms on respiratory organs.
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Interdisciplinary Program in Microbe-Host Biology
  • 批准号:
    10333941
  • 项目类别:
  • 资助金额:
    $31.05万
  • 财政年份:
    2022
  • 负责人:
    RAJENDAR K DEORA
  • 依托单位:
Interdisciplinary Program in Microbe-Host Biology
  • 批准号:
    10681206
  • 项目类别:
  • 资助金额:
    $31.85万
  • 财政年份:
    2022
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Identification of novel immunogenic proteins from Bordetella pertussis
  • 批准号:
    10306163
  • 项目类别:
  • 资助金额:
    $77.88万
  • 财政年份:
    2021
  • 负责人:
    RAJENDAR K DEORA
  • 依托单位:
Identification of novel immunogenic proteins from Bordetella pertussis
  • 批准号:
    10627863
  • 项目类别:
  • 资助金额:
    $76.06万
  • 财政年份:
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