Promethion Mouse Multiplexed Metabolic System

 描述（由申请人提供）：我们寻求资金购买Promethion小鼠多重代谢系统（Promethion）从紫貂系统国际。所要求的仪器是16笼环境受控系统。Promethion使用间接量热法（测量氧气消耗和二氧化碳产生）的概念来估计能量消耗和底物氧化。此外，Promethion能够测量小鼠的实时食物摄入量，水摄入量和运动活动。所要求的仪器的其他升级包括测量体重、家庭笼环境、使用转轮以及使用双食物料斗进行行为研究以及温度和光周期控制。这些升级将满足NIH资助的Sanford-Burnham医学研究所（SBMRI）用户、当地学术合作机构和营利性客户机构对最先进的能量平衡测量日益增长的需求。这些分析对于关注糖尿病和肥胖症等代谢疾病的研究人员特别重要。然而，该仪器也可用于评估其他疾病（如炎症性疾病和癌症）小鼠模型的代谢表型。异丙嗪将纳入SBMRI的心脏代谢表型中心（CMPC）机构。CMPC为内部和外部用户提供啮齿动物模型中复杂的心血管和代谢表型分析服务。对于此应用，所要求的Promethion将服务于4个NIH资助的项目，1个ADA资助的项目以及内部和外部用户尚未确定的项目。SBMRI将为该工具的持续运作提供必要的空间和财政支持。

 DESCRIPTION (provided by applicant): We seek funds to purchase a Promethion Mouse Multiplexed Metabolic System (Promethion) from Sable Systems International. The requested instrument is a 16-cage, environmentally controlled system. The Promethion uses the concepts of indirect calorimetry (measurement of oxygen consumption and carbon dioxide production) to estimate energy expenditure and substrate oxidation. Furthermore, the Promethion has the capacity to measure real-time food intake, water intake and locomotor activity in mice. Additional upgrades in the requested instrument include measurements of body weight, home-cage environment, access to running wheels, and access to dual food hoppers for behavioral studies and temperature and light cycle control. These upgrades will satisfy the growing need for state-of-the-art energy balance measurements by NIH-funded users at the Sanford-Burnham Medical Research Institute (SBMRI), local academic partner institutions and for-profit client institutions. These analyses are of particular relevance to investigators focusing on metabolic diseases such as diabetes and obesity. However, the instrument can also be used to assess metabolic phenotypes in mouse models of other diseases such as inflammatory disease and cancer. The Promethion will be incorporated into the Cardiometabolic Phenotyping Core (CMPC) facility at SBMRI. The CMPC provides internal and external users with access to sophisticated cardiovascular and metabolic phenotyping services in rodent models. For this application, the requested Promethion will serve 4 NIH-funded projects, 1 ADA-funded project and as-of-yet identified projects from internal and external users. SBMRI will provide the necessary space and financial support for the continuous operation of this instrument.