Promethion Mouse Multiplexed Metabolic System

 描述(由申请者提供)：我们寻求资金从紫貂系统国际公司购买扑灭灵鼠多路代谢系统(灭鼠灵)。所要求的仪器是一个16笼的环境控制系统。Promeion使用间接量热法(氧气消耗和二氧化碳产生的测量)的概念来估计能量消耗和底物氧化。此外，灭蚁灵还能够实时测量小鼠的食物摄入量、水分摄入量和运动活动。所要求的仪器的其他升级包括测量体重、家庭笼子环境、使用运行轮，以及使用双食物料斗进行行为研究以及温度和光周期控制。这些升级将满足美国国立卫生研究院资助的桑福德-伯纳姆医学研究所(SBMRI)、当地学术合作机构和营利性客户机构对最先进的能量平衡测量日益增长的需求。这些分析对专注于糖尿病和肥胖症等代谢性疾病的研究人员特别相关。然而，该仪器也可以用于评估其他疾病的小鼠模型的代谢表型，如炎症性疾病和癌症。灭蚁灵将被并入SBMRI的心脏代谢表型核心(CMPC)设施中。CMPC为内部和外部用户提供了啮齿动物模型中复杂的心血管和代谢表型服务。对于这项申请，申请的Prometion将服务于NIH资助的4个项目、ADA资助的1个项目以及来自内部和外部用户的迄今已确定的项目。SBMRI将为该仪器的持续运行提供必要的空间和资金支持。

 DESCRIPTION (provided by applicant): We seek funds to purchase a Promethion Mouse Multiplexed Metabolic System (Promethion) from Sable Systems International. The requested instrument is a 16-cage, environmentally controlled system. The Promethion uses the concepts of indirect calorimetry (measurement of oxygen consumption and carbon dioxide production) to estimate energy expenditure and substrate oxidation. Furthermore, the Promethion has the capacity to measure real-time food intake, water intake and locomotor activity in mice. Additional upgrades in the requested instrument include measurements of body weight, home-cage environment, access to running wheels, and access to dual food hoppers for behavioral studies and temperature and light cycle control. These upgrades will satisfy the growing need for state-of-the-art energy balance measurements by NIH-funded users at the Sanford-Burnham Medical Research Institute (SBMRI), local academic partner institutions and for-profit client institutions. These analyses are of particular relevance to investigators focusing on metabolic diseases such as diabetes and obesity. However, the instrument can also be used to assess metabolic phenotypes in mouse models of other diseases such as inflammatory disease and cancer. The Promethion will be incorporated into the Cardiometabolic Phenotyping Core (CMPC) facility at SBMRI. The CMPC provides internal and external users with access to sophisticated cardiovascular and metabolic phenotyping services in rodent models. For this application, the requested Promethion will serve 4 NIH-funded projects, 1 ADA-funded project and as-of-yet identified projects from internal and external users. SBMRI will provide the necessary space and financial support for the continuous operation of this instrument.