Edge-on/face-on: Trp tripeptides model residue interactions in proteins

边对/面对：Trp 三肽模拟蛋白质中的残基相互作用

• 批准号: 9043904
• 负责人: LAURA Jeanne JUSZCZAK
• 金额: $ 11.78万
• 依托单位: BROOKLYN COLLEGE
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-07-01 至 2017-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9043904
• 关键词: Address; Amines; Amino Acids; Anions; Binding; Cations; Cell physiology; Cells; Charge; Data; Data Analyses; Development; Dipeptides; Disease; Drug Binding Site; Drug Design; Drug Interactions; Electronics; Electrostatics; Environment; Enzymes; Face; Fingerprint; Fluorescence; Fluorescence Spectroscopy; Goals; Head; Health; Human; Image; Indoles; Location; Maps; Measurement; Mechanics; Methods; Mission; Modeling; Molecular; Molecular Conformation; Motion; Outcome Study; Oxidants; Peptides; Personal Satisfaction; Pharmaceutical Preparations; Positioning Attribute; Process; Protein Conformation; Proteins; Relaxation; Research; Resolution; Side; Site; Solvents; Spectrum Analysis; Structure; Techniques; Testing; Time; Tryptophan; United States National Institutes of Health; Vertebral column; Water; Work; absorption; base; carboxylate; conformer; design; drug development; electron donor; fluorescence imaging; improved; molecular dynamics; movie; mutant; nanosecond; optical spectra; protein function; protein structure; quantum; single molecule; tool; user-friendly

DESCRIPTION (provided by applicant): Fluorescence spectroscopy of the amino acid, tryptophan, is a convenient, rapid and user- friendly tool for studying the structure and function of disease-related proteins. The technique is also among the most sensitive. As the diffraction limit to image resolution is breached, imaging of fluorescent single molecules becomes possible. Indeed, single molecule imaging opens the possibility of a movie of the cell, and therefore molecular level understanding of cellular processes. The current level of spectral interpretation for tryptophan, however, is far from the molecular level. This is because tryptophan fluorescence data is complicated by several overlying factors, which include solvent relaxation, multiple conformers, and nonradiative decay. The proposed research addresses the low resolution of data interpretation for tryptophan fluorescence through both molecular dynamics simulation and spectroscopic methods. The goal of the research is to create a matrix of fluorescence spectral parameters that will constitute a 'fingerprint' for specific tryptophan environments and conformations within proteins. With an improvement in spectral interpretation, this work will impact on human health and well-being, both directives of the NIH, because understanding of protein function, and therefore, drug interaction with disease-related proteins, will be greatly improved, cutting the time to drug development.

描述（由申请人提供）：氨基酸色氨酸的荧光光谱法是一种方便、快速和用户友好的工具，用于研究疾病相关蛋白质的结构和功能。这项技术也是最敏感的技术之一。由于突破了图像分辨率的衍射极限，荧光单分子的成像成为可能。事实上，单分子成像打开了细胞电影的可能性，因此可以在分子水平上了解细胞过程。然而，目前对色氨酸的光谱解释水平还远远没有达到分子水平。这是因为色氨酸荧光数据是复杂的几个叠加因素，其中包括溶剂弛豫，多构象异构体，和非辐射衰变。该研究通过分子动力学模拟和光谱方法解决了色氨酸荧光数据解释的低分辨率问题。该研究的目标是创建一个荧光光谱参数矩阵，这些参数将构成蛋白质内特定色氨酸环境和构象的“指纹”。随着光谱解释的改进，这项工作将对人类健康和福祉产生影响，这两项都是NIH的指示，因为对蛋白质功能的理解以及因此与疾病相关蛋白质的药物相互作用将大大改善，从而缩短药物开发时间。