Development of Faster, Lower-Cost, High-Sensitivity Immunoassay for Specific Determination of Glucagon Using EAP-derived Monoclonal Antibodies

 描述（由申请人提供）：胰高血糖素是一种肽激素，在葡萄糖代谢的调节中起核心作用，许多疾病，包括1型和2型糖尿病，都以胰高血糖素水平异常为特征。该领域的临床实践和研究都受到缺乏血液中胰高血糖素水平的适当特异性和灵敏度测定的阻碍。该项目的目标是开发和验证用于胰高血糖素测定的改进的免疫测定法，使用最近制造的胰高血糖素超特异性单克隆抗体。这些检测方法提供的新功能可以使人们对葡萄糖稳态紊乱的理解取得重大进展，同时也可以对这种关键激素进行准确的临床检测。

 DESCRIPTION (provided by applicant): Glucagon is a peptide hormone with a central role in the regulation of glucose metabolism, and many disorders, including Type 1 and Type 2 diabetes, are notable for abnormalities in glucagon levels. Both clinical practice and research in this field are hampered by the lack of appropriately specific and sensitive assays for glucagon levels in blood. The goal of this project is to develop and validate improved immunoassays for glucagon determination, using recently made hyperspecific monoclonal antibodies to glucagon. The new capabilities offered by these assays can lead to significant advances in the understanding of glucose homeostasis disorders and, in parallel, enable accurate clinical testing for this crucial hormone.