Development of Faster, Lower-Cost, High-Sensitivity Immunoassay for Specific Determination of Glucagon Using EAP-derived Monoclonal Antibodies

使用 EAP 衍生的单克隆抗体开发更快、成本更低、高灵敏度的免疫测定法，用于特异性测定胰高血糖素

• 批准号: 9107441
• 负责人: MARK GEISBERG
• 金额: $ 22.01万
• 依托单位: SILVER LAKE RESEARCH CORPORATION
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-08-01 至 2018-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9107441
• 关键词: Affinity; Amino Acid Sequence; Antibodies; Antibody Affinity; Antibody Specificity; Binding; Biological Assay; Blood; Blood specimen; Buffers; Cells; Clinical Research; Data Set; Development; Disease; Enteroglucagon; Enzyme-Linked Immunosorbent Assay; Epitopes; Genes; Glucagon; Goals; Health; Hormones; Human; Immunization; Immunoassay; Immunodominant Epitopes; Label; Lead; Measures; Methodology; Monoclonal Antibodies; Pancreas; Peptides; Performance; Phase; Plasma; Process; Production; Radioactive Tracers; Reagent; Recovery; Regulation; Reporting; Research; Research Design; Role; Sampling; Serum; Silver; Specificity; System; Testing; Time; Universities; Validation; Whole Blood; base; blood glucose regulation; clinical practice; cost; experience; glucose metabolism; immunogenicity; improved; instrumentation; novel; peptide hormone; polypeptide; pre-clinical; preclinical study; prototype; research clinical testing; small molecule; type I and type II diabetes

 DESCRIPTION (provided by applicant): Glucagon is a peptide hormone with a central role in the regulation of glucose metabolism, and many disorders, including Type 1 and Type 2 diabetes, are notable for abnormalities in glucagon levels. Both clinical practice and research in this field are hampered by the lack of appropriately specific and sensitive assays for glucagon levels in blood. The goal of this project is to develop and validate improved immunoassays for glucagon determination, using recently made hyperspecific monoclonal antibodies to glucagon. The new capabilities offered by these assays can lead to significant advances in the understanding of glucose homeostasis disorders and, in parallel, enable accurate clinical testing for this crucial hormone.

 说明(申请人提供)：高血糖素是一种在调节葡萄糖代谢中起核心作用的多肽荷尔蒙，许多疾病，包括1型和2型糖尿病，都是以高血糖素水平异常为特征的。这一领域的临床实践和研究都受到缺乏适当特异和灵敏的血液中胰高血糖素水平分析的阻碍。该项目的目标是开发和验证改进的免疫测定法，使用最近制造的针对胰高血糖素的超特异性单抗来测定胰高血糖素。这些检测提供的新能力可以显著提高对葡萄糖稳态紊乱的理解，同时使这种关键荷尔蒙的准确临床测试成为可能。