In Situ Molecular Analysis

充分利用模式生物需要直接分析相关组织的组织学切片。 由于组织的性质，肌肉骨骼系统中组织的分析在技术上具有挑战性 这需要专门的技术和解剖学知识来获得必要的切片。 核心C的设立提供了技术和咨询资源以及业务结构， 使我们的研究基地能够充分利用基于组织学的技术，并大大提高了 我们的研究人员在肌肉骨骼生物学和医学核心中心的研究计划。 大量的特异性组织化学染色，以及mRNA和蛋白质的原位鉴定， 用于提供我们模型中发育或病理生理事件的详细描述。的 核心的总体目标是为分子鉴定和分析提供组织学服务。 我们的目标组织-骨，软骨，肌肉，肌腱和韧带-在一系列模型中的表型 有机体虽然我们的用户群提交的大部分工作来自转基因小鼠， 我们的目标也是促进其他生物体的组织学分析，包括大鼠，兔子，狗和斑马鱼， 以及人体样本。提供的服务包括组织处理（石蜡，甲基丙烯酸甲酯， 并冷冻）、切片和用一系列技术染色。我们还提供项目咨询 计划和数据分析，以及切片，原位mRNA杂交， 免疫组织化学和定量组织形态计量学。最后，核心提供了整个用户群 与所有核心相关的实验设计和数据分析的适当方法的教育 服务核心的优势在于我们的专业人员，质量控制，以及与客户的密切沟通。 在项目的各个阶段，研究人员都能确保最佳的组织学分析。在最初的4年里， 在这一行动中，核心小组已经适应了其调查员基础的需要，今后将继续这样做 因为这对我们的成功至关重要重要的是，该核心补充了所提出的具体功能分析 核心B和核心D产生的新动物模型。

Full utilization of model organisms requires direct analysis of relevant tissues with histological sections. Analysis of tissues in the musculoskeletal system is technically challenging due to the nature of the tissues involved, which requires specialized techniques and anatomical knowledge to obtain the necessary sections. The creation of Core C has provided the technical and advisory resources and the operational structure to enable our research base to take full advantage of histology-based techniques, and has greatly enhanced the research programs of our investigators in the Core Center for Musculoskeletal Biology and Medicine. A large number of specific histochemical stains, as well as in situ identification of mRNA and proteins, can be employed to provide a detailed picture of developmental or pathophysiological events in our models. The overall objective of the Core is to provide histological services for the identification and analysis of molecular phenotypes of our target tissues - bone, cartilage, muscle, tendon and ligament - in an array of model organisms. Although the bulk of the work submitted by our user base comes from genetically modified mice, we also aim to facilitate histological analysis of other organisms including rat, rabbit, dog and zebrafish, as well as human samples. The services to be offered include tissue processing (paraffin, methylmethacrylate, and frozen), sectioning, and staining with an array of techniques. We also provide consultation for project planning and data analysis, and training for users in sectioning, in situ mRNA hybridization, immunohistochemistry, and quantitative histomorphometry. Finally, the Core provides the entire user base with education on appropriate methods for experimental design and data analysis related to all Core services. The strength of the Core lies in our expert staff, quality control, and close communication with investigators at all stages of their projects to ensure optimal histology-based analysis. In its first 4 years of operation, the Core has adapted to the needs of its investigator base, and this will be continued in the future as it is critical to our success. Importantly, this Core complements the specific functional analyses proposed in Core B and the new animal models generated by Core D.