In Situ Molecular Analysis

原位分子分析

• 批准号: 9031062
• 负责人: DEBORAH J VEIS
• 金额: $ 12.03万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-05-11 至 2019-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9031062
• 关键词: Animal Model; Animals; Area; Arthritis; Biology; Bone Tissue; Canis familiaris; Cartilage; Cells; Communication; Complement; Consultations; Data; Data Analyses; Development; Disease model; Education; Ensure; Event; Experimental Designs; Fracture; Freezing; Frozen Sections; Functional disorder; Funding; Future; Group Meetings; Histology; Human; Imagery; Immunohistochemistry; In Situ; In Situ Hybridization; Knowledge; Label; Ligaments; Medicine; Messenger RNA; Metabolic; Methods; Methylmethacrylate; Modeling; Molecular Analysis; Mus; Muscle; Muscle Weakness; Musculoskeletal; Musculoskeletal Diseases; Musculoskeletal System; Nature; Neoplasm Metastasis; Organism; Oryctolagus cuniculus; Paraffin; Procedures; Proteins; Protocols documentation; Quality Control; Rattus; Research; Research Personnel; Resources; Sampling; Series; Services; Staging; Staining method; Stains; Structure; System; Techniques; Tendon structure; Tissues; Training; Training Programs; Universities; Washington; Work; Zebrafish; base; bone; cost effective; equipment training; laser capture microdissection; molecular phenotype; morphometry; new technology; operation; programs; skeletal; skeletal disorder; success; tissue processing

Full utilization of model organisms requires direct analysis of relevant tissues with histological sections. Analysis of tissues in the musculoskeletal system is technically challenging due to the nature of the tissues involved, which requires specialized techniques and anatomical knowledge to obtain the necessary sections. The creation of Core C has provided the technical and advisory resources and the operational structure to enable our research base to take full advantage of histology-based techniques, and has greatly enhanced the research programs of our investigators in the Core Center for Musculoskeletal Biology and Medicine. A large number of specific histochemical stains, as well as in situ identification of mRNA and proteins, can be employed to provide a detailed picture of developmental or pathophysiological events in our models. The overall objective of the Core is to provide histological services for the identification and analysis of molecular phenotypes of our target tissues - bone, cartilage, muscle, tendon and ligament - in an array of model organisms. Although the bulk of the work submitted by our user base comes from genetically modified mice, we also aim to facilitate histological analysis of other organisms including rat, rabbit, dog and zebrafish, as well as human samples. The services to be offered include tissue processing (paraffin, methylmethacrylate, and frozen), sectioning, and staining with an array of techniques. We also provide consultation for project planning and data analysis, and training for users in sectioning, in situ mRNA hybridization, immunohistochemistry, and quantitative histomorphometry. Finally, the Core provides the entire user base with education on appropriate methods for experimental design and data analysis related to all Core services. The strength of the Core lies in our expert staff, quality control, and close communication with investigators at all stages of their projects to ensure optimal histology-based analysis. In its first 4 years of operation, the Core has adapted to the needs of its investigator base, and this will be continued in the future as it is critical to our success. Importantly, this Core complements the specific functional analyses proposed in Core B and the new animal models generated by Core D.

要充分利用模式生物，需要用组织切片直接分析相关组织。 由于组织的性质，肌肉骨骼系统中的组织分析在技术上是具有挑战性的 这需要专门的技术和解剖学知识来获得必要的切片。 核心C的创建提供了技术和咨询资源以及业务结构，以 使我们的研究基地能够充分利用以组织学为基础的技术，并大大增强了 我们在肌肉骨骼生物学和医学核心中心的研究人员的研究计划。 大量特殊的组织化学染色，以及mRNA和蛋白质的原位鉴定，可以 用于在我们的模型中提供发育或病理生理事件的详细图像。这个 中心的总体目标是为分子鉴定和分析提供组织学服务 我们的目标组织--骨、软骨、肌肉、肌腱和韧带--在一系列模型中的表型 有机体。尽管我们的用户群提交的大部分工作来自转基因小鼠， 我们还致力于促进对其他生物的组织学分析，包括老鼠、兔子、狗和斑马鱼，如 以及人体样本。将提供的服务包括组织加工(石蜡、甲基丙烯酸甲酯、 和冷冻)、切片和用一系列技术进行染色。我们还提供项目咨询服务。 规划和数据分析，以及对用户进行切片、原位mRNA杂交、 免疫组织化学和定量组织形态计量学。最后，核心提供了整个用户群 与所有核心相关的实验设计和数据分析的适当方法的教育 服务。核心的优势在于我们的专业人员，质量控制，以及与客户的密切沟通 研究人员在其项目的所有阶段，以确保最佳的基于组织学的分析。在它的头4年里 在业务方面，核心已经适应了其调查员基础的需要，这将在未来继续下去 因为这对我们的成功至关重要。重要的是，这一核心是对建议的具体功能分析的补充 在核心B和核心D产生的新动物模型中。