Point-of-Care Rapid RNA test for HIV

艾滋病毒的即时快速RNA检测 CrossLife Technologies Inc. 项目总结/摘要 目前的抗体即时检测无法区分母亲和婴儿的 检测婴儿是否感染人类免疫缺陷病毒的抗体 病毒（HIV）。根据卫生组织/艾滋病规划署/儿童基金会最近的一份联合报告， 28%的艾滋病毒阳性母亲所生的婴儿在出生后第一个月内接受了艾滋病毒检测。 两个月[1]。因此，对于新的和改进的诊断存在未满足的需求， 可以应用在护理点检测艾滋病毒感染。一种高度敏感、简单 将开发在护理点检测艾滋病毒的试纸分子测试。的 使用塔拉（模板辅助快速测定）扩增靶序列 该技术由模板依赖性化学转移反应组成， 高周转率、金纳米颗粒-HRP和侧流读出。测试应该是快速的 （<30分钟），并导致出现有色条带，可通过 眼睛或使用智能手机扫描仪捕获。

Point-of-Care Rapid RNA test for HIV CrossLife Technologies Inc. Project Summary/Abstract Current antibody point-of-care tests cannot distinguish between a mother's and baby's antibodies to detect if an infant has become infected with Human Immunodeficiency Virus (HIV). According to a recent joint WHO/UNAIDS/UNICEF report only an estimated 28 percent of babies born to HIV-positive mothers obtained an HIV test within the first two months [1]. Therefore, there are unmet needs for new and improved diagnostics that can be applied at the point-of-care to detect HIV infections. A highly sensitive, simple paper strip molecular test that detects HIV at the point-of-care will be developed. The target sequences are amplified using the TARA (Template-Assisted Rapid Assay) technology which is composed of a template-dependent chemical transfer reaction with high turnover, gold nanoparticle-HRP and lateral flow readout. The test should be rapid (<30 minutes), and results in the appearance of colored bands that are detectable by eye or are captured using a smartphone scanner.