Point-of-Care Rapid RNA test for HIV

艾滋病病毒的照顾点快速RNA检测 CrossLife Technologies Inc. 项目摘要/摘要 目前的抗体护理点检测无法区分母亲和婴儿的 用于检测婴儿是否感染了人类免疫缺陷的抗体 病毒(HIV)。根据世卫组织/联合国艾滋病规划署/联合国儿童基金会最近的一份联合报告，估计 28%的艾滋病毒阳性母亲所生的婴儿在第一个月内接受了艾滋病毒检测 两个月[1]。因此，存在对新的和改进的诊断的未满足的需求 可以在护理地点应用，以检测艾滋病毒感染。一个高度敏感、简单的 将开发在护理地点检测艾滋病毒的纸条分子测试。这个 使用TARA(模板辅助快速分析)扩增靶序列 该技术由模板相关的化学转移反应和 高周转率，金纳米粒子-HRP和侧向流动读出。测试应该是快速的 (&lt；30分钟)，并导致出现可由 眼睛或用智能手机扫描仪捕捉到的。

Point-of-Care Rapid RNA test for HIV CrossLife Technologies Inc. Project Summary/Abstract Current antibody point-of-care tests cannot distinguish between a mother's and baby's antibodies to detect if an infant has become infected with Human Immunodeficiency Virus (HIV). According to a recent joint WHO/UNAIDS/UNICEF report only an estimated 28 percent of babies born to HIV-positive mothers obtained an HIV test within the first two months [1]. Therefore, there are unmet needs for new and improved diagnostics that can be applied at the point-of-care to detect HIV infections. A highly sensitive, simple paper strip molecular test that detects HIV at the point-of-care will be developed. The target sequences are amplified using the TARA (Template-Assisted Rapid Assay) technology which is composed of a template-dependent chemical transfer reaction with high turnover, gold nanoparticle-HRP and lateral flow readout. The test should be rapid (<30 minutes), and results in the appearance of colored bands that are detectable by eye or are captured using a smartphone scanner.