ANIMAL CORE

动物中心为研究者提供了长期内插器械的绵羊以及未内插器械的妊娠和非妊娠动物。动物核心的具体目标是：获得妊娠75天的定时妊娠动物;准备长期器械化的幼龄和成年绵羊用于不同的研究项目;进行尸检并促进组织收集; 并有助于在急性或慢性条件下监测生理变量的体内研究。将妊娠母羊在妊娠80天和81天随机分配接受载体或倍他米松注射，并允许羔羊在足月时自然分娩。 动物核心对三个研究项目至关重要。该中心的主任拥有超过25年的长期使用仪器的胎儿和妊娠动物的工作经验。核心提供了独特的组合的能力，注入生理量的推定调节剂，血浆样品的可用性，在体内记录的生理变量和组织的体外研究 来自已进行体内研究的同一动物。 将对绵羊进行长期仪器化，以获得未处于麻醉应激下的动物的生理数据。我们将使用由Hash，Inc.提供的定期妊娠绵羊。该用品保持一个非常大的羊群，并一直为我们提供健康和准确的怀孕日期 动物所有这些手术和操作将在围产期研究实验室的动物中心内进行。将使用两种性别的妊娠和成年绵羊。贝伐他汀的给药方式与用于刺激未出生的人的肺成熟的给药方式相似，即，两剂醋酸盐和磷酸盐的混合物，间隔24小时。唯一的区别是，我们将根据母体体重进行调整，并给予0.17 mg/kg，最大剂量为12 mg/天。

The Animal Core provides the investigators with chronically instrumented sheep and with non instrumented pregnant and nonpregnant animals. The specific goals of the Animal Core are: to obtain time- dated pregnant animals at 75 days of gestation; to prepare chronically instrumented juvenile and adult sheep for use in the different research projects; to conduct necropsies and facilitate the collection of tissues; and to facilitate the in vivo studies involving monitoring physiologic variables under acute or chronic conditions. Pregnant ewes are randomly assigned to receive either vehicle or betamethasone injections at 80 an 81 days gestation and lambs are allowed to deliver spontaneously at term. The Animal Core is essential for three of the research projects. The director of the core has more than 25 years of experience working with chronically instrumented fetal and pregnant animals. The Core provides the unique combination of the ability to infuse physiological amounts of putative regulators, the availability of plasma samples, in vivo recording of physiological variables and tissues for in vitro studies from the same animal in which in vivo studies have been conducted. Sheep will be chronically instrumented in order to obtain physiological data in animals that are not under the stress of anesthesia. We will use fime dated pregnant sheep provided to us by Hash, Inc. The supplies maintains a very large flock and has consistently provided us with healthy and accurately dated pregnant animals. All of these surgeries and manipulafions will be conducted within the Animal Core in the Perinatal Research Laboratory. Pregnant and adult sheep of both genders will be used. Betamethasone is administered in a manner similar to that used for stimulafing lung maturafion in the unborn human, i.e., two doses of a mixture of the acetate and phosphate form, given 24 hours apart. The only difference is that we will adjust for maternal body weight and give 0.17 mg/kg with a maximum of 12 mg/day.