IGF::OT::IGF ESTABLISHMENT OF CANCER CELL LINES FOR ADVANCING OVARIAN CANCER HEALTH DISPARITY RESEARCH

卵巢癌（OC）不成比例地影响非洲裔美国人（AA）背景的妇女在疾病的各个阶段，从表现到治疗。最令人沮丧的事实是，与高加索美国人（CA）相比，AA患者的死亡率更高，他们的存活率不断下降。由于缺乏合适的细胞系模型，成功地理解和描述现有种族差异的相关机制受到很大阻碍。因此，我们的SBIR I期提案的具体目标是标准化开发新型卵巢癌细胞系的程序和协作设置，以推进卵巢癌健康差异研究。这将通过两个部分来实现：(A)建立卵巢癌体外细胞系实验模型和从AA患者新鲜切除的肿瘤/腹水中提取成纤维细胞；(B)通过同工酶谱分析、核型分析、短串联重复谱分析和突变分析对新发育细胞进行遗传表征。此外，我们将描述发育细胞系的典型形态，生长速度（种群倍增时间），致瘤性和转移潜力以及药物敏感性。该研究的成功完成将为产生新的OC细胞系模型建立一个标准化的方案，从而为我们下一步即从AA背景的患者以及遗传混合物中开发多达50个细胞系模型的第二阶段提供坚实的基础。

Ovarian cancer (OC) disproportionately affects the women of African American (AA) background at all stages of the disease, from presentation through treatment. Most upsetting fact is that AA patients have greater mortality, and their survival rates are continuously decreasing as compared to Caucasian American (CA). Success in understanding and characterizing the involved mechanisms in existing racial disparity is greatly hampered by the lack of suitable cell line models. Therefore, our specific objective of this SBIR Phase I proposal is to standardize the procedure and collaborative set-up for developing novel ovarian cancer cell lines to advance OC health disparity research. This will be achieved in two parts: (A) development of in vitro cell line experimental model of ovarian cancer and fibroblast cells from freshly-resected tumors/ascites fluid of AA patients; (B) Genetic characterization of newly developed cells by isoenzyme profiling, Karyotyping, short-tandem-repeat profiling and mutational analysis. Moreover, we will characterize the developed cell line for typical morphology, growth rates (population doubling time), tumorigenic and metastatic potential and drug sensitivities. Successful completion of the proposed research would establish a standardized protocol for generation of novel cell line models of OC and thus provide a strong foundation for our next step i.e. Phase II for developing up to 50 cell line models from patients of AA backgrounds as well as genetic admixtures.