Microbiome and Ocular Inflammatory Disease

微生物组和眼部炎症疾病

• 批准号: 9362419
• 负责人: Hatice Sen
• 金额: $ 14.43万
• 依托单位: NATIONAL EYE INSTITUTE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/9362419
• 关键词: 18 year old; Age related macular degeneration; Blood specimen; Categories; Clinical; Collection; Data; Disease; Enrollment; Evaluation; Feces; Goals; Inflammatory; Liquid substance; Outcome; Participant; Patients; Population Control; Protocols documentation; RNA; Sampling; Specimen; Techniques; Uveitis; deep sequencing; healthy volunteer; microbial; microbiome; peripheral blood; rectal; research clinical testing

What precipitates ocular inflammatory episodes remains unknown, but a possible potentiating factor is the microbiome. The microbiome has become increasingly studied with the advent of new techniques, but these have not been applied to uveitis. We wish to evaluate microbiome composition in patients with the ocular inflammatory diseases: uveitis and age-related macular degeneration (AMD) who may have a recent onset untreated disease or be on standard therapy. A total of 65 participants will be enrolled in this study. Participants must be at least 18 years old. Microbiome samples from an existing control population of healthy volunteers established by Dr. Jonathan Braun of UCLA will be used as a comparison for the microbiome samples obtained from the participants in this study. Peripheral blood samples from an existing control population of healthy volunteers established at the NEI will be used as a comparison for the peripheral blood samples obtained from the participants in this study. This is an observational, cross-sectional, single-center study. Participants will receive a complete ocular examination with clinical testing as determined clinically and will undergo an anoscopy for collection of rectal fluid specimens or will submit a stool sample. The outcomes will be related to the comparison of the various microbiome samples to controls and between groups. In addition, comparisons will be made to these findings and the immunome.

是什么促使眼部炎症发作仍然未知，但一个可能的增强因素是微生物组。随着新技术的出现，微生物组的研究越来越多，但这些尚未应用于葡萄膜炎。我们希望评估患有眼部炎症性疾病的患者的微生物组组成：葡萄膜炎和年龄相关性黄斑变性（AMD），这些患者可能最近患有未经治疗的疾病或正在接受标准治疗。本研究将入组共计65例受试者。参加者必须年满18岁。来自加州大学洛杉矶分校的Jonathan Braun博士建立的健康志愿者现有对照人群的微生物组样本将用于比较从本研究参与者中获得的微生物组样本。来自NEI建立的现有健康志愿者对照人群的外周血样本将用于本研究受试者外周血样本的比较。 这是一项观察性、横断面、单中心研究。受试者将接受完整的眼部检查和临床确定的临床测试，并将接受肛门镜检查以收集直肠液样本或提交粪便样本。 结果将与各种微生物组样品与对照组和组间的比较有关。此外，将对这些发现和免疫组进行比较。